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EC number: 700-321-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011-02-08 to 2011-02-23
- Reliability:
- 1 (reliable without restriction)
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Deviations:
- no
- Principles of method if other than guideline:
- NA
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- yes
Test material
- Reference substance name:
- hexazinc(2+) bis(2-hydroxy-3,5-bis[(1R)-1-phenylethyl]benzoate) bis(2-hydroxy-3,5-bis[(1S)-1-phenylethyl]benzoate) 2-hydroxy-3-(1-phenylethyl)-5-(1-{4-[(1R)-1-phenylethyl]phenyl}ethyl)benzoate 2-hydroxy-3-(1-phenylethyl)-5-(1-{4-[(1S)-1-phenylethyl]phenyl}ethyl)benzoate 2-hydroxy-3-[(1R)-1-phenylethyl]benzoate 2-hydroxy-3-[(1S)-1-phenylethyl]benzoate 2-hydroxy-5-(1-phenylethyl)-3-(1-{4-[(1R)-1-phenylethyl]phenyl}ethyl)benzoate 2-hydroxy-5-(1-phenylethyl)-3-(1-{4-[(1S)-1-phenylethyl]phenyl}ethyl)benzoate 2-hydroxy-5-[(1R)-1-phenylethyl]benzoate 2-hydroxy-5-[(1S)-1-phenylethyl]benzoate
- EC Number:
- 700-321-2
- Molecular formula:
- C30H26O6Zn, C46H42O6Zn, C62H58O6Zn
- IUPAC Name:
- hexazinc(2+) bis(2-hydroxy-3,5-bis[(1R)-1-phenylethyl]benzoate) bis(2-hydroxy-3,5-bis[(1S)-1-phenylethyl]benzoate) 2-hydroxy-3-(1-phenylethyl)-5-(1-{4-[(1R)-1-phenylethyl]phenyl}ethyl)benzoate 2-hydroxy-3-(1-phenylethyl)-5-(1-{4-[(1S)-1-phenylethyl]phenyl}ethyl)benzoate 2-hydroxy-3-[(1R)-1-phenylethyl]benzoate 2-hydroxy-3-[(1S)-1-phenylethyl]benzoate 2-hydroxy-5-(1-phenylethyl)-3-(1-{4-[(1R)-1-phenylethyl]phenyl}ethyl)benzoate 2-hydroxy-5-(1-phenylethyl)-3-(1-{4-[(1S)-1-phenylethyl]phenyl}ethyl)benzoate 2-hydroxy-5-[(1R)-1-phenylethyl]benzoate 2-hydroxy-5-[(1S)-1-phenylethyl]benzoate
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Laboratoire Elevage Janvier, B.P. 4105, Route des Chênes Secs, 53940 Le Genest-St-Isle CEDEX FRANCE
- Age at study initiation: 10-11 weeks
- Weight at study initiation: 231 – 238 g
- Fasting period before study: overnight fast prior to dosing
- Housing: in groups of 3 animals, in polycarbonate cages
- Diet: laboratory rodent diet, ad libitum
- Water: ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature: 22 + -3 °C
- Humidity: 24-64 % *)
- Photoperiod: 12 hrs dark / 12 hrs light
*) The relative humidity (24-64%) was slightly out of the target range (30-70%) during the study. This deviation has no impact on the outcome of the study and interpretation of the results.
IN-LIFE DATES: From: 2011-01-13 to 2011-02-23
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- DMSO
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle/Amount administered: 200 mg/mL in Dimethyl Sulfoxide (DMSO); dosing volume of 10 mL/kg bw.
- Justification for choice of vehicle: selected as a suitable vehicle for the study purposes based on test item properties and trial formulations
- Batch No. /Lot No. (DMSO): BCBC5888V
MAXIMUM DOSE VOLUME APPLIED: 2000 mg/kg bw
CLASS METHOD
- Rationale for the selection of the starting dose:
The initial dose level was selected as the one which is most likely to produce mortality in some of the dosed animals. In the lack of any preliminary toxicological information, 2000 mg/kg bw was selected to be the starting dose.
Initially, three female animals were treated with 2000 mg/kg bw of Reaction Product of 2-Hydroxybenzoic Acid, Styrene and Oxozinc. No mortality was observed, therefore further 3 animals were treated at the dose level of 2000 mg/kg bw. One animal died in this second dose group, further testing was not required according to the test guidelines (OECD 423, Commission Regulation (EC) No 440/2008, B.1.tris). - Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 3 females/groups
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
All animals were weighed on allocation to the study (Day -1), immediately prior to
dosing (Day 0), on Day 7 and prior to necropsy.
Further examinations performed:
Clinical signs: Animals were observed for clinical signs immediately upon dosing, and approximately at 30 minutes, 1, 2, 3, 4 and 6 hours after dosing and daily thereafter for a total of 14 days. - Statistics:
- NA
Results and discussion
- Preliminary study:
- NA
Effect levels
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- Reaction Product of 2-hydroxybenzoic acid, styrene and oxozinc caused mortality in one animal at a dose level of 2000 mg/kg bw.
- Clinical signs:
- other: Treatment with Reaction Product of 2-hydroxybenzoic acid, styrene and oxozinc caused decreased activity (5/6), in coordination (1/6) and general body tone (1/6) during the 14 days observation period
- Gross pathology:
- Amber coloured mucoid material found in the digestive content of the small intestines was considered to be test item-related in the found dead animal. No macroscopic observations were noted in remaining animals at a dose level of 2000 mg/kg bw.
- Other findings:
- No other findings.
Any other information on results incl. tables
No other information
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Under the conditions of this study, the acute oral LD50 value of the test item Reaction Product of 2-hydroxybenzoic acid, styrene and oxozinc was found to be above 2000 mg/kg bw in female RjHan:WI rats.
- Executive summary:
In an acute oral toxicity study according to OECD TG 423 (2001) and under GLP, 2 groups/3 females of fasted, young adult Sprague-Dawley rats were given a single oral dose of the test substance in Dimethyl Sulfoiyde (DMSO) at 2000 mg/kg bw and consecutively observed for 14 days.
Initially, 3 females (Group 1) were treated at a dose level of 2000 mg/kg bw. As no mortality was observed, a confirmatory group (Group 2) was treated at the same dose level. One animal died in the confirmatory group, therefore no further testing was required according to OECD 423 (2001) and Commission Regulation (EC) No 440/2008. No mortality occurred and no signs of toxicity were observed during the 14 day observation period. All animals showed slight clinical signs of piloerection and production of soft faeces 4 hours after dosing. Recovery had occurred within 24 hours and no other signs of reaction to treatment were apparent during the 14 day observation period. The bodyweight gain was not impaired. Thus, the oral LD50 was determined to be >2000 mg/kg bw.
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