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Diss Factsheets
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EC number: 203-311-1 | CAS number: 105-58-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data from Peer reviewed publication
Data source
Reference
- Reference Type:
- publication
- Title:
- ICCVAM Test Method Evaluation Report. The Reduced Murine Local Lymph Node Assay: An Alternative Test Method Using Fewer Animals to Assess the Allergic Contact Dermatitis Potential of Chemicals and Products
- Author:
- NIH
- Year:
- 2 009
- Bibliographic source:
- NIH Publication No. 09-6439. National Institutes of Health
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Principles of method if other than guideline:
- The protocol for the reduced LLNA is identical to that of the traditional LLNA with one exception. In the traditional LLNA, three dose levels of each test substance are tested, while in the rLLNA only the highest dose level that does not induce systemic toxicity and/or excessive skin irritation is tested for skin sensitizing activity. The term “limit dose”, sometimes used to refer to the rLLNA, accurately depicts a modified LLNA that tests only the highest dose level that does not induce local irritation and/or systemic toxicity. The terms “cut-down” and “reduced” LLNA also accurately describe the reduction in the number of doses tested and emphasize the reduction in the number of animals used to perform the test.
The rLLNA measures lymphocyte proliferation after topical exposure to a potential skin sensitizing substance. The test substance is administered topically on three consecutive days to the ears of mice at a concentration that provides maximum solubility of the test substance without systemic toxicity and/or excessive local irritation. Two days after the final application of the test substance, 3H-thymidine or [125]I-iododeoxyuridine (in phosphate-buffered saline; 250 uL/mouse) is administered via the tail vein. Five hours later the draining auricular lymph nodes are excised, and a single-cell suspension from the lymph nodes of each animal is prepared for quantifying the incorporation of radioactivity, which correlates with lymph node cell proliferation. The incorporation of radioactive 3H-thymidine or [125]I-iododeoxyuridine for each mouse is expressed in disintegrations per minute (dpm). The SI is calculated as the ratio of the mean dpm/mouse for each treatment group against the mean dpm/mouse for the vehicle control group. The threshold for a positive response is an SI of >= 3. - GLP compliance:
- not specified
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Dimethyl carbonate
- EC Number:
- 210-478-4
- EC Name:
- Dimethyl carbonate
- Cas Number:
- 616-38-6
- IUPAC Name:
- dimethyl carbonate
- Test material form:
- other: liquid
- Details on test material:
- not further specified
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals and environmental conditions:
- not further specified
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 0.5, 5 and 50%
- No. of animals per dose:
- no data
- Details on study design:
- The protocol for the reduced LLNA is identical to that of the traditional LLNA with one exception. In the traditional LLNA, three dose levels of each test substance are tested, while in the rLLNA only the highest dose level that does not induce systemic toxicity and/or excessive skin irritation is tested for skin sensitizing activity. The term “limit dose”, sometimes used to refer to the rLLNA, accurately depicts a modified LLNA that tests only the highest dose level that does not induce local irritation and/or systemic toxicity. The terms “cut-down” and “reduced” LLNA also accurately describe the reduction in the number of doses tested and emphasize the reduction in the number of animals used to perform the test.
The rLLNA measures lymphocyte proliferation after topical exposure to a potential skin sensitizing substance. The test substance is administered topically on three consecutive days to the ears of mice at a concentration that provides maximum solubility of the test substance without systemic toxicity and/or excessive local irritation. Two days after the final application of the test substance, 3H-thymidine or [125]I-iododeoxyuridine (in phosphate-buffered saline; 250 uL/mouse) is administered via the tail vein. Five hours later the draining auricular lymph nodes are excised, and a single-cell suspension from the lymph nodes of each animal is prepared for quantifying the incorporation of radioactivity, which correlates with lymph node cell proliferation. The incorporation of radioactive 3H-thymidine or [125]I-iododeoxyuridine for each mouse is expressed in disintegrations per minute (dpm). The SI is calculated as the ratio of the mean dpm/mouse for each treatment group against the mean dpm/mouse for the vehicle control group. The threshold for a positive response is an SI of >= 3. - Positive control substance(s):
- not specified
- Statistics:
- Yes
Results and discussion
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: The calculated stimulation indices were given with 0.64, 0.69 and 1.71
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: no details reported
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Remarks:
- Migrated information
- Conclusions:
- Dimethyl carbonate was tested in a reduced LLNA (the protocol for the rLLNA is identical to that of the traditional LLNA with one exception, as only the highest dose level that does not induce systemic toxicity and/or excessive skin irritation is tested for skin sensitizing activity), where concentrations of 0.5, 5 and 50% have been tested. The calculated stimulation indices were given with 0.64, 0.69 and 1.71, i.e. the substance gave no indications for a skin sensitizing potential.
- Executive summary:
Dimethyl carbonate was tested in a reduced LLNA (the protocol for the rLLNA is identical to that of the traditional LLNA with one exception, as only the highest dose level that does not induce systemic toxicity and/or excessive skin irritation is tested for skin sensitizing activity), where concentrations of 0.5, 5 and 50% have been tested. The calculated stimulation indices were given with 0.64, 0.69 and 1.71, i.e. the substance gave no indications for a skin sensitizing potential.
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