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EC number: 800-765-8 | CAS number: 1424149-03-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013-01-23 to 2013-02-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study, no deficiencies
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- sodium/triethanolamine 4-((2-hydroxyethyl)amino)-3-pentaproenyl-4-oxobutanoate
- EC Number:
- 800-765-8
- Cas Number:
- 1424149-03-0
- Molecular formula:
- C21H40NO4.1/2Na.1/2C6H15NO3
- IUPAC Name:
- sodium/triethanolamine 4-((2-hydroxyethyl)amino)-3-pentaproenyl-4-oxobutanoate
- Details on test material:
- - Name of test material (as cited in study report): Pentapropylensuccinic anhydride, reaction products with ethanolamine, sodium and triethanolamine salts
- Physical state: Brown, viscous liquid
- Analytical purity: 100 % (w/w)
- Purity test date: 2011-11-15
- Lot/batch No.: ESD0010887
- Expiration date of the lot/batch: 2013-12-13
- Stability under test conditions: Not applicable
- Storage condition of test material: Room temperature, protected from light, in original container
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0.500 - 1.00 - 2.00 - 4.00 - 8.00 mg/L
- Sampling method: ll concentration levels of the test item and the control were analysed by LC-MS/MS at the start of the exposure intervals on days 0, 9, 14 (0 h) and at the end of the exposure intervals on days 2, 12, 16 (48 or 72 h). For the analyses of the old media, separate replicates without daphnids and algae were prepared and stored under test conditions until sampling.
- Sample storage conditions before analysis: All original samples were stored at room temperature until sample preparation. Prepared samples were stored at room temperature until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution (8.00 mg/L of the test item were weighed out) was freshly prepared with dilution water at the start of the test period and at every water renewal day.Dispersion treatment was agitation.
- Eluate: Dilution water
- Differential loading: 0.500 – 1.00 – 2.00 – 4.00 – 8.00 mg/L
- Controls: 10 replicates of dilution water without test item.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain/clone: Clone 5
- Justification for species other than prescribed by test guideline: Daphnia magna STRAUS is recommended in the guideline
- Source: Own breeding (Origin: Institut fuer Wasser- Boden- und Lufthygiene)
- Age of parental stock (mean and range, SD): > 14 days
- Feeding during test
- Food type: Mix of Pseudokirchneriella subcapitata and Desmodesmus subspicatus
- Amount: 0.2 mg C/daphnia per day
- Frequency: renewals 3 times per week
ACCLIMATION
- Acclimation period: 2 h in dilution water
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: During acclimation the daphnids were not fed
- Feeding frequency: None
- Health during acclimation (any mortality observed): Healthy
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: The parent animals were removed from the culture medium and the juveniles collected over a sieve and flushed into fresh medium
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Post exposure observation period:
- Not observed
Test conditions
- Hardness:
- Total Hardness [mg/L] as CaCO3
Nominal
test item
concentration
[mg/L] I F I F I F
Day 0 Day 2 Day 9 Day 12 Day 14 Day 16
2013-01-30 2013-02-01 2013-02-08 2013-02-11 2013-02-13 2013-02-15
8.00 174 173 Not determined due to 100 % mortality
4.00 Not determined 176 180 148 148
Control 176 171 178 155 171 174 - Test temperature:
- Temperature [°C]
Nominal
test item
concentration
[mg/L] I F I F I F
Day 0 Day 2 Day 9 Day 12 Day 14 Day 16
2013-01-30 2013-02-01 2013-02-08 2013-02-11 2013-02-13 2013-02-15
8.00 21.2 20.6 Not determined due to 100 % mortality
4.00 Not determined 19.1 19.9 20.6 19.6
Control 20.8 20.7 19.5 19.9 19.2 19.9 - pH:
- pH-Values
Nominal
test item
concentration
[mg/L] I F I F I F
Day 0 Day 2 Day 9 Day 12 Day 14 Day 16
2013-01-30 2013-02-01 2013-02-08 2013-02-11 2013-02-13 2013-02-15
8.00 8.40 8.01 Not determined due to 100 % mortality
4.00 Not determined 8.10 7.69 7.58 7.67
Control 8.53 8.40 8.36 7.59 8.00 8.05 - Dissolved oxygen:
- Dissolved Oxygen Concentration [mg/L]
Nominal
test item
concentration
[mg/L] I F I F I F
Day 0 Day 2 Day 9 Day 12 Day 14 Day 16
2013-01-30 2013-02-01 2013-02-08 2013-02-11 2013-02-13 2013-02-15
8.00 8.79 7.98 Not determined due to 100 % mortality
4.00 Not determined 8.89 8.26 8.76 7.49
Control 8.70 8.52 9.09 7.73 9.09 8.96 - Salinity:
- Not measured, freshwater
- Nominal and measured concentrations:
- Please refer to "Any other information on materials and methods"
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed with parafilm
- Material, size, headspace, fill volume: 100 mL glass beaker, filled with 50 mL test solution
- Aeration: No
- Renewal rate of test solution (frequency): 3 x per week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4, according to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light : 8 h dark per day
- Light intensity: Max. 20 µE*m-2*s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Adult mortality: daily
- Number of juveniles: daily
- Stillborn juveniles and aborted eggs: daily
- Appearance of first brood: between days 8 and 11
- Intrinsic rate of natural increase: test end
- Growth (total length and dry weight): test end
RANGE-FINDING STUDY
Based on the results of the acute immobilisation test (NOACK Lab-ID: 120214CB/DAI14963) conducted at the test facility, the definitive reproduction test was carried out with 5 concentration levels of the test item in the range of 0.500 to 8.00 mg/L, prepared with dilution water as specified below. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- - Mortality of parent animals:
The test item induced biologically significant adult mortality of 100 % at the concentration level of 8.00 mg/L during the test period of 21 days. In the concentration levels of 0.500 to 4.00 mg/L and in the control, all parental daphnids survived until the end of the test after 21 days.
The EC50 for the adult mortality was calculated to be 5.66 mg/L (95% confidence limits: 4.00 – 8.00 mg/L).
- No. of offspring produced per day per female:
The number of juveniles in all replicates of the control and the test groups was counted and recorded every day.
The average number of living juveniles per surviving parental daphnid at the end of the test after 21 days was 86 in the control group. The reproductive output was statistically significant reduced in comparison to the control at the concentration level of 4.00 mg/L (One Way Analysis of Variance, DUNNETT’S method, p = 0.05).
At the concentration levels of 0.500 to 2.00 mg/L, the reproductive output was comparable to the reproductive output at the control. Due to 100 % adult mortality, no offspring was observed at the concentration level of 8.00 mg/L.
Based on the significant reduction of the reproductive output at the concentration level of 4.00 mg/L, the No Observed Effect Concentration (NOEC) after 21 days was assessed at 2.00 mg/L and the Lowest Observed Effect Concentration (LOEC) was assessed at 4.00 mg/L. An EC50-value for the reduction of the reproductive output could not be calculated, because the reduction was limited to a maximum of 14 % of the control value within the tested concentration range.
- Body length and weight of parent animals:
The mean values of the body length of the surviving parental daphnids in the tested concentration levels of 0.500 to 4.00 mg/L were determined to be in the range of 5.13 to 5.38 mm per daphnid, 5.23 mm per daphnid in the control group.
The mean dry body weight was determined to be in the range of 1.14 to 1.24 mg per daphnid in the concentration levels 0.500 to 4.00 mg/L and 1.21 mg per daphnid in the control.
- Type and number of morphological / behavioural abnormalities:
please refer to the respective table
- Time to first brood release or time to hatch:
The first brood was released between days 8 and 11 by all parental daphnids of the control and the concentration levels of 0.500 to 4.00 mg/L. At the tested concentration levels of 0.500 to 4.00 mg/L and in the control, 4 broods were produced by the surviving parental daphnids. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 (24 h) = 1.69 (CI 1.41 - 2.12) mg/L - Reported statistics and error estimates:
- The EC50-value for the reproductive output after 21 days could not be calculated, because the reduction was limited to a maximum of 14 % of the control value within the tested concentration range. The EC50-value for the reference item was calculated by sigmoidal dose-response regression. The 95 % confidence limits for the EC-values were calculated from the best-fit values, the standard error and the t-distribution with the software GraphPad prism5. The EC50-value for the adult mortality after 21 days was calculated as the geometric mean of the highest concentration level causing 0 % adult mortality and the lowest concentration level causing 100 % adult mortality, because only 0 and 100 % adult mortality was observed. These concentration levels were used as confidence limits. The EC10- and the EC100-value for the adult mortality deduced directly from the observation data without calculation.
Any other information on results incl. tables
Number of Juveniles in the Control and Test Groups after 21 Days
Nominal test item conc. [mg/L] |
No. of parents prod. juv. |
Mean no. of Juveniles per parent producing juveniles |
Comparison versus control |
||||||||||||||
Number of living juveniles in replicate no. |
Total no. |
CV |
|||||||||||||||
Red. |
Stat. |
||||||||||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
å |
N |
MV ± SD |
[%] |
[%] |
|||
8.00 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
n.a. |
0 |
n.a. |
n.a. |
n.a. |
n.a. |
|
4.00 |
63 |
75 |
89 |
68 |
77 |
61 |
74 |
74 |
81 |
82 |
744 |
10 |
74 ± |
9 |
12 |
14 |
yes |
2.00 |
94 |
70 |
81 |
74 |
64 |
85 |
71 |
78 |
80 |
96 |
793 |
10 |
79 ± |
10 |
13 |
8 |
no |
1.00 |
84 |
77 |
74 |
97 |
79 |
83 |
64 |
69 |
74 |
80 |
781 |
10 |
78 ± |
9 |
12 |
10 |
no |
0.500 |
85 |
75 |
80 |
79 |
71 |
83 |
76 |
78 |
67 |
82 |
776 |
10 |
78 ± |
6 |
7 |
10 |
no |
Control |
91 |
95 |
78 |
83 |
86 |
82 |
86 |
86 |
91 |
85 |
863 |
10 |
86 ± |
5 |
6 |
— |
— |
First Appearance of Living Juveniles in the Individual Groups
Nominal test item concentration [mg/L] |
Day of first appearance of living juveniles |
First |
Mean |
|||||||||
in replicate no. |
appearance |
number |
||||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
mean day |
of broods |
|
8.00 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
n.a. |
n.a. |
4.00 |
8 |
8 |
9 |
9 |
11 |
9 |
8 |
8 |
8 |
10 |
8.8 |
4 |
2.00 |
8 |
9 |
10 |
9 |
8 |
8 |
8 |
8 |
8 |
8 |
8.4 |
4 |
1.00 |
8 |
8 |
9 |
9 |
9 |
9 |
9 |
9 |
9 |
8 |
8.7 |
4 |
0.500 |
9 |
8 |
9 |
9 |
9 |
9 |
8 |
8 |
9 |
9 |
8.7 |
4 |
Control |
9 |
8 |
9 |
9 |
8 |
9 |
9 |
8 |
9 |
8 |
8.6 |
4 |
Relative Number of Dead (Stillborn + Aborted Eggs) to Total Number of Juveniles
Nominal test item concentration [mg/L] |
Number of juveniles |
Percentage of dead juveniles#) [%] |
||
Dead |
Alive |
Total |
||
8.00 |
n.a. |
n.a. |
n.a. |
n.a. |
4.00 |
39 |
744 |
783 |
5 |
2.00 |
36 |
793 |
829 |
4 |
1.00 |
2 |
781 |
783 |
1 |
0.500 |
17 |
776 |
793 |
2 |
Control |
1 |
863 |
864 |
1 |
Mortality [%] of the Adult Daphnids after 7, 14 and 21 Days of Exposure
(n = 10)
Nominal Test Item Concentration [mg/L] |
Adult Mortality [%] |
||
7 days |
14 days |
21 days |
|
8.00 |
1001) |
1001) |
1001) |
4.00 |
0 |
0 |
0 |
2.00 |
0 |
0 |
0 |
1.00 |
0 |
0 |
0 |
0.500 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
1) = biologically significant mortality (> 20%)
Total Body Length of the Parental Daphnids
Nominal test item concentration [mg/L] |
Total length of the parent animals [mm] |
MV |
N |
Dry weight |
||||||||||
Replicate no. |
||||||||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
[mm] |
S |
MV |
||
8.00 |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
n.a. |
0 |
n.a. |
n.a. |
4.00 |
4.75 |
5.50 |
5.50 |
5.50 |
5.00 |
5.75 |
5.75 |
5.75 |
5.25 |
5.00 |
5.38 |
10 |
12.4 |
1.24 |
2.00 |
5.25 |
5.00 |
5.25 |
5.25 |
5.00 |
5.50 |
5.00 |
5.50 |
5.00 |
5.25 |
5.20 |
10 |
12.3 |
1.23 |
1.00 |
5.75 |
5.25 |
5.25 |
5.25 |
5.25 |
5.00 |
5.00 |
4.75 |
5.00 |
4.75 |
5.13 |
10 |
11.4 |
1.14 |
0.500 |
4.75 |
5.25 |
5.00 |
5.25 |
5.00 |
5.00 |
5.25 |
5.25 |
5.50 |
5.50 |
5.18 |
10 |
11.9 |
1.19 |
Control |
5.75 |
5.25 |
5.25 |
5.00 |
5.50 |
5.00 |
5.25 |
5.50 |
4.75 |
5.00 |
5.23 |
10 |
12.1 |
1.21 |
MV = mean value
N = number of parental daphnids alive
-- = not applicable, due to mortality of the parental daphnid
n.a. = not applicable, due to 100 % adult mortality
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The significant reduction of the reproductive output at the concentration level of 4.00 mg/L was the most sensitive effect in this study. Therefore, the No Observed Effect Concentration (NOEC) after 21 days was assessed at 2.00 mg/L and the Lowest Observed Effect Concentration (LOEC) was assessed at 4.00 mg/L.
An EC50-value for the reduction of the reproductive output could not be calculated, because the reduction was limited to a maximum of 14 % of the control value within the tested concentration range.
The EC50-value for the adult mortality was calculated to be 5.66 mg/L (95% confidence limits: 4.00 – 8.00 mg/L). The EC100-value for the adult mortality was 8.00 mg/L.
Endpoints assessed Pentapropylensuccinic anhydride, reaction products with ethanolamine, sodium and triethanolamine salts
Nominaltest item concentration
[mg/L]
EC50 Adult Mortality
(with 95 % confidence limits) 5.66 (4.00 – 8.00)
EC100 Adult Mortality 8.00
NOECReproduction 2.00 mg/L
LOECReproduction 4.00 mg/L - Executive summary:
The Daphnia magna Reproduction Test (semi-static, 21 d) of the test item Pentapropylensuccinic anhydride, reaction products with ethanolamine, sodium and triethanolamine salts (batch number:ESD0010887) was conducted according to OECD 211 (2008) from 2013-01-23 to 2013-02-21, with the definitive exposure phase from 2013-01-30 to 2013-02-20, at Dr.U.Noack-Laboratorien, Käthe-Paulus-Str. 1, 31157 Sarstedt, Germany.
Test species was Daphnia magna STRAUS (Clone 5). Ten daphnids, individually held, were used per concentration level, and control. At test start the daphnids were 2 to 24 hours old. The study was carried out undersemi-static conditions with renewal of the test solutions three times per week. Aim of the Daphnia Reproduction Test over 21 days was to assess effects on the reproduction capacity and other test item-related effects or parameters such as time of production of first brood, adult mortality, intrinsic rate of natural increase, occurrence of aborted eggs and stillborn juveniles and body length of the parental daphnids.
Nominal concentrations of Pentapropylensuccinic anhydride, reaction products with ethanolamine, sodium and triethanolamine salts were selected as follows: 0.500 - 1.00 - 2.00 - 4.00 - 8.00 mg/L.
The concentrations of the test item Pentapropylensuccinic anhydride, reaction products with ethanolamine, sodium and triethanolamine salts were analytically verified by LC-MS/MS of samples taken at the start of the exposure intervals on days 0, 9, 14 (0 h) and at the end of the exposure intervals on days 2, 12, 16 (48 or 72 h) of all concentration levels and the control.
The measured concentrations of the test item at the start of the exposure intervals were in the range of 102 to 144 % of the nominal values. At the end of the exposure intervals (48 or 72 h), the measured concentrations of the test item were in the range of 105 to 139 % of the nominal values. The effect levels are based on the nominal concentrations, because the recoveries in the range of 102 – 144 % of the nominal values demonstrate, that the daphnids were fully exposed to the test item concentrations throughout the test period.
· The average number of living juveniles per surviving parental daphnid at the end of the test after 21 days was 86 in the control group. The reproductive output was statistically significant reduced in comparison to the control at the concentration level of 4.00 mg/L (One Way Analysis of Variance, Dunnett’smethod, p = 0.05). At the concentration levels of 0.500 to 2.00 mg/L, the reproductive output was comparable to the reproductive output at the control. Due to 100 % adult mortality, no offspring was observed at the concentration level of 8.00 mg/L.
An EC50-value for the reduction of the reproductive output could not be calculated, because the reduction was limited to a maximum of 14 % of the control value within the tested concentration range.· Based on the significant reduction of the reproductive output at the concentration level of 4.00 mg/L, the No Observed Effect Concentration (NOEC) after 21 days was assessed at 2.00 mg/L and the Lowest Observed Effect Concentration (LOEC) was assessed at 4.00 mg/L.
· The test item induced biologically significant adult mortality of 100 % at the concentration level of 8.00 mg/L during the test period of 21 days.In the concentration levels of 0.500 to 4.00 mg/L and in the control, all parental daphnids survived until the end of the test after 21 days. The EC50for the adult mortality was calculated to be 5.66 mg/L (95% confidence limits: 4.00 – 8.00 mg/L).
· The coefficient of variation of the number of living offspring produced per parent was 6 % in the control. In the test groups the coefficient of variation was in the range of 7 to 13 %.
· No stillborn juveniles and 1 aborted egg were observed in the control group during the exposure period of 21 days. Related to the total number of produced juveniles (dead + alive) the percentage of dead juveniles was in the range of 1 to 5 % in the tested concentration levels of 0.500 to 4.00 mg/L.
· The intrinsic rates of natural increase (IR) of the surviving parental daphnids accounting for generation time and number of offspring were used for calculation of population growth and maintenance. The intrinsic rates of natural increase determined for the surviving daphnids at the concentration levels of 0.500 to 4.00 mg/L were comparable to the IR at the control (One Way Analysis of Variance, Dunnett’s method, p = 0.05).
· The first brood was released between days 8 and 11 by all parental daphnids of the control and the concentration levels of 0.500 to 4.00 mg/L. At the tested concentration levels of 0.500 to 4.00 mg/L and in the control, 4 broods were produced by the surviving parental daphnids.
· The mean values of the body length of the surviving parental daphnids in the tested concentration levels of 0.500 to 4.00 mg/L were determined to be in the range of 5.13 to 5.38 mm per daphnid, 5.23 mm per daphnid in the control group.
The mean dry body weight was determined to be in the range of 1.14 to 1.24 mg per daphnid in the concentration levels 0.500 to 4.00 mg/L and 1.21 mg per daphnid in the control.
· No males or ephippia (winter eggs) were observed in the control or in the test groups.
· Water quality parametersas pH-value, dissolved oxygen, water hardness and temperature were determined to be within the acceptable limits.
· In order to prove the validity of the test system and test conditions at the test facility, an acute immobilisation test according to DIN 38412 L 11 is monthly carried out with potassium dichromate as reference item.
The EC50 of the reference item was 1.69 mg/L (95% confidence limits: 1.41 - 2.12 mg/L) after 24 hours and therefore within the prescribed concentration range of 1.0 - 2.5 mg/L of quality criteria according to AQS P 9/2 (05/1996) for daphnids clone 5 cultured in Elendt M4 medium. The EC50-value of the reference item is also within the recommended range of 0.6 - 2.1 mg/L according to OECD-Guideline 202.Test Item Related Effects (NOEC, LOEC, EC10, EC50 and EC100)
(based on the nominal concentrations of the test item)
Effects
Pentapropylensuccinic anhydride, reaction products
with ethanolamine, sodium and triethanolamine saltsNominal test item concentrations [mg/L]
Control
0.500
1.00
2.00
4.00
8.00
Mean Number of Living Juveniles per
Producing Parent (Reproduction Rate ± SD)
86 ± 5
78 ± 6
78 ± 9
79 ± 10
74 ± 91)
---
Coefficient of Variation of the Mean Number of Juveniles per Producing Parent [%]
6
7
12
13
12
---
Mean Number of Broods
4.00
4.00
4.00
4.00
4.00
---
Appearance of First Brood [Mean Day]
8.6
8.7
8.7
8.4
8.8
---
Mean Intrinsic Rates of Natural Increase
0.45
0.43
0.43
0.44
0.43
---
Percentage of Dead Juveniles Related to the Total Number of Juveniles [%]
1
2
1
4
5
---
Parental Daphnids: Mean Dry Weight [mg]
1.21
1.19
1.14
1.23
1.24
---
Parental Daphnids: Mean Body Length [mm]
5.23
5.18
5.13
5.20
5.38
---
Adult Mortality after 21 Days [%]
0
0
0
0
0
1002)
EC50 Adult Mortality
5.66 mg/L (95 % confidence limits: 4.00 – 8.00 mg/L)
EC100 Adult Mortality
8.00 mg/L
NOECReproduction
2.00 mg/L
LOECReproduction
4.00 mg/L
SD = standard deviation
1) = statistical significance (One Way Analysis of Variance, Dunnett’s method, p = 0.05)
2) = significant adult mortality (> 20 %)
--- = not applicable, due to 100 % adult mortality
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