Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 941-432-8 | CAS number: 1085706-46-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 9th, 2007 to August 9th 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Done by OECD and GLP standards
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.42 (Skin Sensitisation: Local Lymph Node Assay)
- GLP compliance:
- yes
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- tert-butyl (2S)-2-[5-(4'-{2-[(2S)-1-[(tert-butoxy)carbonyl]pyrrolidin-2-yl]-1H-imidazol-5-yl}-[1,1'-biphenyl]-4-yl)-1H-imidazol-2-yl]pyrrolidine-1-carboxylate
- EC Number:
- 941-432-8
- Cas Number:
- 1085706-46-2
- Molecular formula:
- C36 H44 N6 O4
- IUPAC Name:
- tert-butyl (2S)-2-[5-(4'-{2-[(2S)-1-[(tert-butoxy)carbonyl]pyrrolidin-2-yl]-1H-imidazol-5-yl}-[1,1'-biphenyl]-4-yl)-1H-imidazol-2-yl]pyrrolidine-1-carboxylate
- Test material form:
- solid: crystalline
- Details on test material:
- Off white crystalline solid stored at room temperature in the dark.
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
- Details on test animals and environmental conditions:
- At the start of the study the mice were in the weight range of 15-23 grams and were 8-12 weeks old. Animals were acclimatised for at least 5 days prior to the study. Free access to mains tap water and food was allowed through out the study. The temperature and relative humidity were set to
achieve limits of 19-25C and 30-70% respectively. The lighting was controlled by a time switch to give 12 hours continuous light and 12 hours of
darkness.
Study design: in vivo (LLNA)
- Vehicle:
- dimethylformamide
- Concentration:
- Test material at concentrations of 5%, 10% or 25% w/w.
- No. of animals per dose:
- 3 groups of five animals were treated at each concentration group and a further group of five animals were treated as a control group with
dimethylformamide alone. - Details on study design:
- The mice were treated by daily application of 25 μl of the appropriate concentration of the test material to the dorsal surface of each ear for three
consecutive days (Days 1, 2, 3) by using a micropipette whose tip is used to spread the formulation over the dorsal surface of each ear. A further
group of five mice received the vehichle in the same manner. On Day 6 all mice were injected with 250 ul of a phophate buffered saline solution
containing 3H-methyl thymidine (3HTdR ) to the tail vein giving a total of 20 uCi to each mouse. All animals were observed twice daily on Days 1, 2
and 3 and once daily on days 4 ,5, and 6. Any signs of toxicity or ill health were noted. Body weights of each mouse were recorded before dosing and before termination. Five hours after administration of 3HTdR all mice were killed by carbon dioxide asphyxiation and their auricular lymph nodes were excised and a 1 ml of phosphate buffered saline was added to each set of lymph nodes. Preparation of a single cell suspension was completed for the lymph nodes cells for each individual animal following standard procedures. Determination of the 3HTdR incorporation was completed by measuring radioactive disintegration for each animal's lymph node cells by using a beta-scintillation counter. - Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
- Statistics:
- Analysis of variance (ANOVA) was carried out on the data followed by Dunnett's test for comparisons made beween the control and treatment groups in the event of a significant result from the ANOVA.
Results and discussion
- Positive control results:
- Hexylcinnamaldehyde, Tech 85% was considered to be a sensitiser under the conditions of the test.
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: see Remark
- Remarks:
- Concentration of test material in the vehicle at 5 % resulted in a stimulation index (SI) of 1.05 which is a negative result.Concentration of test material in the vehicle at 10 % resulted in a stimulation index (SI) of 1.66 which is a negative result.Concentration of test material in the vehicle at 25 % resulted in a stimulation index (SI) of 2.51 which is a negative result. A stimulation index of less than 3 was recorded for the three concentrations of the test material.
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: see Remark
- Remarks:
- Concentration of test material in the vehicle resulted in a mean disintegration of 861.87 dpm. Concentration of test material in the vehicle at 5 % resulted in a mean disintegration of 903.70 dpm which is a negative result. Concentration of test material in the vehicle at 10 % resulted in a mean disintegration of 1426.65 dpm which is a negative result.Concentration of test material in the vehicle at 25 % resulted in a mean disintegration of 2167.13 dpm which is a negative result.The results of the statistical analysis of the data indicated there was no significant difference between the control group and the test groups.
Any other information on results incl. tables
There were no deaths. No signs of systemic toxicity were noted in the test or control animals during the test. White residual test material was noted, post dose one to three days after treatment on the ears of animals treated with the test material at a concentration of 10% w/w in dimethyl formamide and post dose on Days 1 and 2 and on Days 3 and 4 in animals treated with the test material at a concentration of 25% w/w in dimethyl formamide. |
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The test material was considered to be a non-sensitiser under the conditions of the test. A stimulation index of less than 3 was recorded for the threeconcentrations of the test material.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.