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EC number: 941-628-3 | CAS number: 1263184-87-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 Apr 2015 to 17 Apr 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: MEP, P.R. China, the Guideline for the Testing of Chemicals, Effects on Biotic Systems (the Second Edition), No. 203 "Fish Acute Toxicity Test''
- Version / remarks:
- 2013
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- During the test, three samples were collected from each test vessel and one from the control group in the freshly prepared media at 0 and 48 hours and in the expired media at 48 and 96 hours.
- Vehicle:
- yes
- Remarks:
- Acetone
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Appropriate weights of the test item (0.39642g, 0.53654g, 0.72410g, 0.97786g and 1.32147g for each test solution) were dissolved in 25 mL acetone to obtain test item solutions of 15.0, 20.3, 27.4, 37.0 and 50.0 mg/mL (taking into account the analytical purity of the test item). 10 mL of each acetone solution was applied into dry glass bottles and the bottles were rotated to allow the acetone to evaporate, thus leaving a thin coating of test item on the inner surface. Afterwards, 10 L of test water was added into the glass bottles and stirred magnetically for 1.5 hrs at 450 rpm to obtain the test solutions. The test was performed with renewal of the test solutions every 48 hours.
- Controls: Blank control and positive control
- Concentration of vehicle in test medium: Acetone was evaporated from the test vessel before introduction of test organisms. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Length at study initiation: 2.1 - 2.6 cm (sample of 5 random fish before test start)
- Weight at study initiation: 0.262 - 0.351 g (sample of 5 random fish before test start)
ACCLIMATION
- Acclimation period: At least 12 days
- Acclimation conditions: Water quality, light conditions and temperature were the same as the test conditions
- Type and amount of food during acclimation: Not reported
- Feeding frequency during acclimation: Twice on weekdays and once over the weekend until 24 hours before the start of the test
- Health during acclimation (any mortality observed): No signs of disease or mortalities during the initial 12 day acclimation period
FEEDING DURING TEST: None - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- None reported
- Hardness:
- 136 mg CaCO3/L
- Test temperature:
- 21.85 - 24.30 °C
- pH:
- 7.11 - 8.18
- Dissolved oxygen:
- 69.7 - 98.3 % ASV (air saturation value)
- Salinity:
- Not applicable
- Conductivity:
- Not reported
- Nominal and measured concentrations:
- - Nominal concentrations: 15, 20.3, 27.4, 37.0 and 50 mg/L
- Mean measured concentrations: 14.39, 19.42, 24.69, 30.96 and 38.01 mg/L (corresponding to 95.9, 95.7, 90.1, 83.7 and 76.0 % of the nominal concentrations). - Details on test conditions:
- TEST SYSTEM
- Test vessel: Tanks (30 x 30 x 20 cm)
- Material, size, headspace, fill volume: Glass tanks filled with 10 L test solution
- Aeration: The water was aerated for more than 24 hours to attain dissolved oxygen saturation before use
- Renewal rate of test solution: Every 48 hours
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: < 1.0 g fish/L water
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Groundwater treated with Aquapro Pure Water System
- Total organic carbon: < 2 mg/L
- Metals: < 0.001 mg/L As, < 0.001 mg/L Cd, < 0.004 mg/L Cr(6+), < 0.005 mg/L Pb, < 0.0001 mg/L Hg, < 0.001 mg/L Se, < 0.03 mg/L Al, < 0.05 mg/L Fe, < 0.05 Mn, < 0.0075 Cu, < 0.05 mg/L Zn
- Intervals of water quality measurement: Dissolved oxygen and pH were measured daily and temperature was recorded in 1 hr intervals
OTHER TEST CONDITIONS
- Adjustment of pH: None reported
- Photoperiod: 12 hours light: 12 hours darkness
- Light intensity: Not reported
EFFECT PARAMETERS MEASURED: Mortality and toxic signs (e.g. loss of equilibrium, swimming behaviour, respiratory function, pigmentation, etc.) were recorded at 3, 24, 48, 72 and 96 hours after exposure. The criteria for mortality were no visible movement (e.g. gill movement) and no reaction to touching of the caudal peduncle.
TEST CONCENTRATIONS
- Range finding study: In a non-GLP range-finding test using the concentrations of 5, 10, 20 and 50 mg/L, the test fish at 20 mg/L showed toxic symptoms such as erratic swimming, lethargy, discoloration. The test fish at 50 mg/L showed 100% mortality. It was assumed that the LC50 may be in the range of 20 - 50 mg/L.
- Test concentrations: 5, 10, 20 and 50 mg/L
- Results used to determine the conditions for the definitive study: At 20 mg/L, fish showed toxic symptoms such as erratic swimming, lethargy and discoloration. At 50 mg/L, 100 % mortality was observed. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 25.87 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95 % CL: 23.89 - 28.01 mg/L
- Details on results:
- - Behavioural abnormalities: at test concentrations of 19.42 mg/L and above, visible toxic signs were observed, such as erratic swimming, lethargy and discolouration.
- Mortality of control: No deaths occurred in the control group during the 96-hour exposure period.
- Other adverse effects control: No visible toxic signs were observed in the control group during the 96-hour exposure period. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- LC50: 277.0 mg/L (24 hours) - Reported statistics and error estimates:
- The 96-hour LC50 and the 95 % confidence limits were derived by Trimmed Spearman-Karber method using mean measured concentrations.
- Sublethal observations / clinical signs:
Cumulative mortality and toxic signs of Danio rerio exposed to the test item
Nominal concentration (mg/L) Mean measured concentration (mg/L) Initial number of exposed fish Cumulative mortality 3 h 24 h 48 h 72 h 96 h Control - 7 0 0 0 0 0 15 14.39 7 0 0 0 0 0 20.3 19.42 7 0 (7sd) 0 (7sld) 0 (7sld) 0 (7sld) 0 (7sld) 27.4 24.69 7 0 (7sd) 1 (6sld) 2 (5sld) 2 (5sld) 2 (sld) 37.0 30.96 7 0 (7sld) 7 - - - 50 38.01 7 2 (5sld) 7 - - - The number of fish with toxic signs are given in parentheses:
s: erratic swimming
l: lethargy
d: discolouration
- Validity criteria fulfilled:
- yes
- Remarks:
- see "any other information on materials and methods incl. tables".
- Conclusions:
- The 96-hour LC50 for Danio rerio exposed to the test item was determined to be 25.87 mg/L (95 % confidence limits: 23.89 - 28.01 mg/L).
- Executive summary:
A 96-hour acute toxicity study of the test item was conducted with zebra fish (Danio rerio) in a semi-static system according to OECD Guideline Number 203 (1992). Fish were exposed to nominal test item concentrations of 15, 20.3, 27.4, 37.0 and 50 mg/L (corresponding to mean measured test item concentrations of 14.39, 19.42, 24.69, 30.96 and 38.01 mg/L), alongside a blank control. Test solutions were renewed at 48 hours. The 96-hour LC50 was determined to be 25.87 mg/L (95 % confidence limits: 23.89 - 28.01 mg/L). No death or visible signs of toxicity were observed in the control group or treatment groups up to 14.39 mg/L. At 19.42 mg/L and above, visible toxic signs were observed, such as erratic swimming, lethargy and discolouration.
Reference
Description of key information
96-h LC50 = 25.87 mg/L, in freshwater zebrafish (Danio rerio), OECD 203, Xue 2015.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 25.87 mg/L
Additional information
A 96-hour acute toxicity study of the test item was conducted with zebra fish (Danio rerio) in a semi-static system according to OECD Guideline Number 203 (1992). Fish were exposed to nominal test item concentrations of 15, 20.3, 27.4, 37.0 and 50 mg/L (corresponding to mean measured test item concentrations of 14.39, 19.42, 24.69, 30.96 and 38.01 mg/L), alongside a blank control. Test solutions were renewed at 48 hours. The 96-hour LC50 was determined to be 25.87 mg/L (95 % confidence limits: 23.89 - 28.01 mg/L). No death or visible signs of toxicity were observed in the control group or treatment groups up to 14.39 mg/L. At 19.42 mg/L and above, visible toxic signs were observed, such as erratic swimming, lethargy and discolouration.
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