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EC number: 477-690-9 | CAS number: 874819-71-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2005-02-07 - 2005-08-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- OECD 201 (1984)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- Test solutions of the chosen concentrations were prepared by mixing a stock solution of the test item with test medium and inoculum culture.
Before test initiation, a stock solution was prepared by dissolving the test item in the test medium. Then, adequate volumes of the stock solution were mixed with test medium to obtain the desired test concentrations. The test solutions were prepared just before introduction of the algae (= test initiation). The same volume of algal inoculum was added to each test solution. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
freshwater green algae - Desmodesmus subspicatus
source: Albrecht-v.-Haller-Institut fur Pflanzenwissenschaften; Sammlung von Algenkulturen Nikolausberger Weg 18, D-37073 Gottingen
strain: 86.81 SAG- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- no
- Test temperature:
- 23.5 - 24.1 degree C
- pH:
- 8
- Nominal and measured concentrations:
- nominal (mg/l): 3.13, 6.25, 12.5, 25.0, 50.0, 100.0
- Reference substance (positive control):
- not required
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.25 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 51.4 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 22 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- yes
- Conclusions:
- The growth rate in the 100 mg/l treatment as 92% inhibited compared to the control. A NOEC of 6.25 mg/l and a LOEC of 12.5 mg/l have been estimated for the parameters cell growth and growth rate. The EC50 was calculated as 28.3 mg/l (cell growth) and 51.4 mg/l (growth rate).
- Executive summary:
The test was performed according to the recommendations of the OECD Guideline 201 (1984).
The purpose of this study was to determine the inhibitory effect of the test item on the growth of the fresh water unicellular green alga Desmodesmus subspicatus. Exponentially growing cultures of this algal species were exposed to various concentrations of the test item under defined conditions. The aim of the test was to determine the EbC50 (cell biomass), ErC50 (growth rate), LOEC and NOEC for the endpoint based on D. subspicatus growth (cell number) over a period of 72 hours. Other endpoints included cell size, colour differences or aggregation of alga cells.
The growth rate in the 100 mg/l treatment as 92% inhibited compared to the control. A NOEC of 6.25 mg/l and a LOEC of 12.5 mg/l have been estimated for the parameters cell growth and growth rate. The EC50 was calculated as 28.3 mg/l (cell growth) and 51.4 mg/l (growth rate).
Reference
Description of key information
OECD 202 guideline study, GLP: NOEC: 6.25 mg/l
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 51.4 mg/L
- EC10 or NOEC for freshwater algae:
- 6.25 mg/L
Additional information
A study was performed to assess the effect of the test material on the growth of the green alga Scenedesmus subspicatus. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No 201, "Alga, Growth Inhibition Test".
The growth rate in the 100 mg/l treatment as 92% inhibited compared to the control. A NOEC of 6.25 mg/l and a LOEC of 12.5 mg/l have been estimated for the parameters cell growth and growth rate. The EC50 was calculated as 28.3 mg/l (cell growth) and 51.4 mg/l (growth rate).
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