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EC number: 604-045-2 | CAS number: 137862-53-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Apr - Sep 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- First test
Sampling: Frequency
At the beginning (fresh) and at the end (spent) of three Intervals of 48 hours (nominal days o and 2, 7 and 9, 12 and 14) and one interval of 72 hours (nominal days 2 and 5). The 'spent' solutions were pooled before sampling.
2 ml from the approximate centre of the tost vessel.
Second test
Sampling: Frequency
At the beginning (fresh) and at the end (spent) of four intervals of 48 hours (nominal days O and 2, 7 and 9, 12 and 14, 19 and 21) and one interval of 72 hours
(nominal days 2 and 5). The 'spent' solutions were pooled before sampling.
2 ml from the approximate centre of the test vessel. - Vehicle:
- yes
- Remarks:
- M7, as prescribed by Dr: Elendt-Schneider (Elendt, .B.·P ., 1990: Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus. Protoplasma 154, 25-33)
- Details on test solutions:
- The standard test procedures required generation of test solutions, which should contain completely dissolved test substance concentrations or stable and homogeneous mixtures or dispersions. The testing of concentrations that disturb the test system should be prevented (e.g. precipitate or a film of the test substance on the water surface).
The batch of VAA489 VAL tested was a white powder with a purity of 99 % and completely soluble at the concentrations tested. Preparation started by adding weighed amounts of test substance to the test medium in 2-litre measuring flasks followed by treatment with ultra-sonic waves for 10 to 20 minutes. Then the flasks were filled until the mark and treated with ultrasonic waves for another 10 to 20 minutes, until all test substance had completely dissolved.
Test solutions prepared at 10 mg/I were used to prepare lower concentrations by subsequent dilution. All final test solutions were clear and colourless.
Test concenll'ations
First test
VAA4S9VAL 10 mg/I
Control Test medium without test substance or other additives (0 mg/I).
Second test
VM4B9VAL 1.0, 1.8, 3.2 and 5.6 mg/I
Controls Test medium without test substance or other additives (0 mg/I). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna (Crustacea, Cladocera) (Straus, 1820), at least third generation, obtained by acyclical parthenogenesis under specified breeding conditions
Daphnids originated from a healthy stock. 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of males, ephippia or discoloured
animals and there was no delay In the production of the first brood.
For the test seiection of young daphnia with an age of < 24 hours, from parental daphnids of more than two weeks old. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Remarks on exposure duration:
- Flrst test: 19d Second test: 21 d
- Hardness:
- between 13 and 16 DH
- Test temperature:
- 19.6 and 20.9°C In the first test and between
19.6 and 20.3°C in the second test - pH:
- Flrst test: 7.7 to 8.1
Second test: 7.6 and 8.6 - Dissolved oxygen:
- > 7 mg/I
- Nominal and measured concentrations:
- The concentrations of VAA489 VAL measured in the freshly prepared solutions were in agreement with nominal and thus confirmed the repeatability of the preparation of the test concentrations. Measured concentrations remained stable during all renewal periods, both 48 and 72-hour. The concentrations measured in samples taken from 10 mg/I ranged from 94 to 104 % of nominal. The concentraiions measured in the samples taken during the second test ranged betwee 86 and 116 % of nominal. Therefore all results could be based on nominal concentrations.
- Reference substance (positive control):
- no
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 5.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 5.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the condilions of the present study V M489 VAL did not affect survival, reproduction or growth of Daphnia magna sfgnificantly at concantrations up to and including 5.6 mg/I after 21days of exposure (NOEC). The LOEC of VAA489 VAL for survival and reproduction of Daphnia magna was 10 mg/I, the regulatory limit concentration.
Reference
Description of key information
Under the condilions of the present study V M489 VAL did not affect survival, reproduction or growth of Daphnia magna sfgnificantly at concantrations up to and including 5.6 mg/I after 21days of exposure (NOEC). The LOEC of VAA489 VAL for survival and reproduction of Daphnia magna was 10 mg/I, the regulatory limit concentration.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 5.6 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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