Registration Dossier
Registration Dossier
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EC number: 227-410-4 | CAS number: 5831-80-1
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline Study with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- N,N-dibutyloleamide
- EC Number:
- 227-410-4
- EC Name:
- N,N-dibutyloleamide
- Cas Number:
- 5831-80-1
- Molecular formula:
- C26H51NO
- IUPAC Name:
- N,N-Dibutyloleamide
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 15 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 37
- Sampling time:
- 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
The test item must be regarded as not readily biodegradable after 28 days.- Executive summary:
The ready biodegradability was determined with a non adapted activated sludge for the test item over a test period of 28 days in the CO2 Evolution test. The study was conducted according to OECD 301B. The test item was tested with a concentration of 15 mg/L in duplicates, corresponding to a carbon content (TOC) of 11.9 mgC/L in the test vessels.
To check the activity of the test system sodium benzoate was used as functional control.
The10% level (beginning of biodegradation) was reached after 11 days. The pass level of 60 % was not reached. The biodegradation of the test item came to a maximum of 37 % after 28 days. %.
The validity criteria according to the guideline are fulfilled.
The test item must be regarded as not readily biodegradable after 28 days.
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