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EC number: 210-155-8 | CAS number: 608-25-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Title:
- Unnamed
- Year:
- 2 008
Materials and methods
Test guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-methylresorcinol
- EC Number:
- 210-155-8
- EC Name:
- 2-methylresorcinol
- Cas Number:
- 608-25-3
- Molecular formula:
- C7H8O2
- IUPAC Name:
- 2-methylbenzene-1,3-diol
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat S9 fraction
- Test concentrations with justification for top dose:
- 33 to 5000 microgram/plate
- Vehicle / solvent:
- ethanol
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Additional information on results:
- The Ames-test was performed with Salmonella typhimurium strains TA98, TA100, TA102,
TA1535 and TA1537 with and without fortified rat liver post-mitochondrial fraction (S9 mix)
induced with phenobarbital and β-naphthoflavone. The substance was tested in ethanol (>99%) at
six concentrations in the range of 33 to 5,000 μg/plate. This range was based upon the
results of a pre-experiment. The main assay was performed in two independent
experiments both with and without liver microsomal activation. A direct plate incorporation
assay as well as a pre-incubation experiment as the second run of the main test were
performed. Sodium azide (10 μg/plate) served as a positive control for TA100 and TA1535,
4-nitro-o-phenylene-diamine (10 μg/plate) for TA1537 and TA98 and methyl methane
sulfonate (4 μg/plate) for TA102 without S9 mix. The enzyme activity of S9 mix was
separately controlled by testing with 2-aminoanthracene (2.5 μg/plate) in all tester strains.
The solvent ethanol and the untreated fresh cell suspension served as negative controls.
Results
Irregular background growth was observed at 5000 μg/plate in strain TA1537 without S9
mix and in strain TA102 with S9 mix in the second experiment. Toxic effects, evident as a
reduction in the number of revertants, were observed in strain TA1537 without S9 mix in
experiment I, and in strains TA1537, TA98, and TA100 with S9 mix and strains TA98 and
TA102 without S9 mix in experiment II. No substantial increase in revertant colony numbers
of any of the five tester strains was observed following treatment with the substance at any dose
level, either in the presence or absence of metabolic activation. There was also no tendency
of higher mutation rates with increasing concentrations in the range below the generally
acknowledged border of biological relevance. The positive controls used showed a distinct
increase of induced revertant colonies.
Conclusion
The substance was not considered to be mutagenic in the in vitro bacterial mutagenicity test in the
presence or absence of S9 mix.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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