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EC number: 216-223-3 | CAS number: 1530-32-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- of read across substance
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two toxicity study of aquatic invertebrate for the test chemical :
1 and 2) Toxicity of test material on aquatic invertebrate Pomacea canaliculata was evaluated
3) To evaluate short term toxicity of aquatic invertebrate on test material - GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- other: 1 and 2) Pomacea canaliculata , 3) Daphnia magna
- Details on test organisms:
- 1 and 2) -common name: Golden apple snails
- Method of breeding: Aquaria with dimensions of 50 cm x 25 cm x 35 cm were used for maintaining and breeding snails in the laboratory. The top of the aquarium was covered with nylon screen. Lamps were positioned 15 cm above the aquaria with a cycle of 16 hrs “on”, and 8 hrs “off’. The aquarium floor was covered with a layer of small pebbles, and was filled with water to a depth of 25 cm. Tap water reduced the thickness of the snail shell and the number of eggs, dechlorinated tap water caused the snails to secrete mucus. As a result, fresh aerated well water (pH 7.5,
26 °C) was provided in a continuous flow. Wild adult snails, with a diameter greater than 5 cm, were collected from the field. After copulation, fresh eggs were incubated and the young snails were hatched in 10-17 days. The young snails were fed sweet potato leaves in the aquarium for 65-70 days until maturity.
The breeding was repeated and the third generation snails of 35-40 days old were used for toxicity testing. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
- Nominal and measured concentrations:
- 1 and 2) 0.5 to 250 mg/l
- Details on test conditions:
- 1 and 2) - Test vessel: 500 mL glass bottles
-fill volume: 450 ml
- No. of organisms per vessel: 30
- No. of vessels per concentration (replicates): 6 - Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 16.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: linear regression (r2)= 2.441x + 8.742(0.942)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 67.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: linear regression (r2)= 0.617 x + 8.382(0.932)
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 52.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The test chemical is likely to be toxic to aquatic invertebrate atleast in the concentration range of 16 mg/l to 67 mg/l
- Executive summary:
Data available for the test chemical chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate .The studies are as mentioned below:
1) Short term toxicity to aquatic invertebrate Pomacea canaliculata (golden apple snail) was evaluated for the test material. Aquaria with dimensions of 50 cm x 25 cm x 35 cm were used for maintaining and breeding snails in the laboratory. The top of the aquarium was covered with nylon screen. Lamps were positioned 15 cm above the aquaria with a cycle of 16 hrs “on”, and 8 hrs “off’. The aquarium floor was covered with a layer of small pebbles, and was filled with water to a depth of 25 cm. Tap water reduced the thickness of the snail shell and the number of eggs, dechlorinated tap water caused the snails to secrete mucus. As a result, fresh aerated well water (pH 7.5, 26 °C) was provided in a continuous flow. Wild adult snails, with a diameter greater than 5 cm, were collected from the field. After copulation, fresh eggs were incubated and the young snails were hatched in 10-17 days. The young snails were fed sweet potato leaves in the aquarium for 65-70 days until maturity.The breeding was repeated and the third generation snails of 35-40 days old were used for toxicity testing.
The bioassay was conducted in 500 mL glass bottles, each was tilled with thirty snails and 450 mL of water. The concentration of test compounds ranged from 0.5 to 250 mg/L (6 different concentrations, three replications). After 72 hrs exposure, the number of dead snails were recorded. The snail was considered to be dead if there was no response to touch, the operculum shut tight, but with no resistance when the operculum was lifted, and the foot was out and swollen.The lethal effect concentration ( LC50) was determined to be 16.9 mg/l(linear regression (r2)= 2.441x + 8.742(0.942)). Based on the above effect concentration it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.
2)Short term toxicity to aquatic invertebrate Pomacea canaliculata (golden apple snail) was evaluated for the test material. Aquaria with dimensions of 50 cm x 25 cm x 35 cm were used for maintaining and breeding snails in the laboratory. The top of the aquarium was covered with nylon screen. Lamps were positioned 15 cm above the aquaria with a cycle of 16 hrs “on”, and 8 hrs “off’. The aquarium floor was covered with a layer of small pebbles, and was filled with water to a depth of 25 cm. Tap water reduced the thickness of the snail shell and the number of eggs, dechlorinated tap water caused the snails to secrete mucus. As a result, fresh aerated well water (pH 7.5, 26 °C) was provided in a continuous flow. Wild adult snails, with a diameter greater than 5 cm, were collected from the field. After copulation, fresh eggs were incubated and the young snails were hatched in 10-17 days. The young snails were fed sweet potato leaves in the aquarium for 65-70 days until maturity. The breeding was repeated and the third generation snails of 35-40 days old were used for toxicity testing.
The bioassay was conducted in 500 mL glass bottles, each was tilled with thirty snails and 450 mL of water. The concentration of test compounds ranged from 0.5 to 250 mg/L (6 different concentrations, three replications). After 72 hrs exposure, the number of dead snails were recorded. The snail was considered to be dead if there was no response to touch, the operculum shut tight, but with no resistance when the operculum was lifted, and the foot was out and swollen. The lethal effect concentration ( LC50) was determined to be 67.4 mg/l linear regression (r2)= 0.617 x + 8.382(0.932). Based on the above effect concentration it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.
3) Short term toxicity test for aquatic invertebrtae was performed for the test material for 24 h . The EC50 value was observed to be 52.6 mg/l , The EC0 was noted at 31.2 mg/l and the EC100 was observed in the concentration 125 mg/l.Based on the above effect concentartions it can be considered that test material was toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.
Based on the above effect concentartions it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.
Reference
Description of key information
Short term toxicity to aquatic invertebrates:
Data available for the test chemical chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate .The studies are as mentioned below:
1) Short term toxicity to aquatic invertebrate Pomacea canaliculata (golden apple snail) was evaluated for the test material. Aquaria with dimensions of 50 cm x 25 cm x 35 cm were used for maintaining and breeding snails in the laboratory. The top of the aquarium was covered with nylon screen. Lamps were positioned 15 cm above the aquaria with a cycle of 16 hrs “on”, and 8 hrs “off’. The aquarium floor was covered with a layer of small pebbles, and was filled with water to a depth of 25 cm. Tap water reduced the thickness of the snail shell and the number of eggs, dechlorinated tap water caused the snails to secrete mucus. As a result, fresh aerated well water (pH 7.5, 26 °C) was provided in a continuous flow. Wild adult snails, with a diameter greater than 5 cm, were collected from the field. After copulation, fresh eggs were incubated and the young snails were hatched in 10-17 days. The young snails were fed sweet potato leaves in the aquarium for 65-70 days until maturity.The breeding was repeated and the third generation snails of 35-40 days old were used for toxicity testing.
The bioassay was conducted in 500 mL glass bottles, each was tilled with thirty snails and 450 mL of water. The concentration of test compounds ranged from 0.5 to 250 mg/L (6 different concentrations, three replications). After 72 hrs exposure, the number of dead snails were recorded. The snail was considered to be dead if there was no response to touch, the operculum shut tight, but with no resistance when the operculum was lifted, and the foot was out and swollen.The lethal effect concentration ( LC50) was determined to be 16.9 mg/l(linear regression (r2)= 2.441x + 8.742(0.942)). Based on the above effect concentration it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.
2)Short term toxicity to aquatic invertebrate Pomacea canaliculata (golden apple snail) was evaluated for the test material. Aquaria with dimensions of 50 cm x 25 cm x 35 cm were used for maintaining and breeding snails in the laboratory. The top of the aquarium was covered with nylon screen. Lamps were positioned 15 cm above the aquaria with a cycle of 16 hrs “on”, and 8 hrs “off’. The aquarium floor was covered with a layer of small pebbles, and was filled with water to a depth of 25 cm. Tap water reduced the thickness of the snail shell and the number of eggs, dechlorinated tap water caused the snails to secrete mucus. As a result, fresh aerated well water (pH 7.5, 26 °C) was provided in a continuous flow. Wild adult snails, with a diameter greater than 5 cm, were collected from the field. After copulation, fresh eggs were incubated and the young snails were hatched in 10-17 days. The young snails were fed sweet potato leaves in the aquarium for 65-70 days until maturity. The breeding was repeated and the third generation snails of 35-40 days old were used for toxicity testing.
The bioassay was conducted in 500 mL glass bottles, each was tilled with thirty snails and 450 mL of water. The concentration of test compounds ranged from 0.5 to 250 mg/L (6 different concentrations, three replications). After 72 hrs exposure, the number of dead snails were recorded. The snail was considered to be dead if there was no response to touch, the operculum shut tight, but with no resistance when the operculum was lifted, and the foot was out and swollen. The lethal effect concentration ( LC50) was determined to be 67.4 mg/l linear regression (r2)= 0.617 x + 8.382(0.932). Based on the above effect concentration it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.
3) Short term toxicity test for aquatic invertebrtae was performed for the test material for 24 h . The EC50 value was observed to be 52.6 mg/l , The EC0 was noted at 31.2 mg/l and the EC100 was observed in the concentration 125 mg/l.Based on the above effect concentartions it can be considered that test material was toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.
Based on the above effect concentartions it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 16.9 mg/L
Additional information
Short term toxicity to aquatic invertebrates:
Data available for the test chemical chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate .The studies are as mentioned below:
1) Short term toxicity to aquatic invertebrate Pomacea canaliculata (golden apple snail) was evaluated for the test material. Aquaria with dimensions of 50 cm x 25 cm x 35 cm were used for maintaining and breeding snails in the laboratory. The top of the aquarium was covered with nylon screen. Lamps were positioned 15 cm above the aquaria with a cycle of 16 hrs “on”, and 8 hrs “off’. The aquarium floor was covered with a layer of small pebbles, and was filled with water to a depth of 25 cm. Tap water reduced the thickness of the snail shell and the number of eggs, dechlorinated tap water caused the snails to secrete mucus. As a result, fresh aerated well water (pH 7.5, 26 °C) was provided in a continuous flow. Wild adult snails, with a diameter greater than 5 cm, were collected from the field. After copulation, fresh eggs were incubated and the young snails were hatched in 10-17 days. The young snails were fed sweet potato leaves in the aquarium for 65-70 days until maturity.The breeding was repeated and the third generation snails of 35-40 days old were used for toxicity testing.
The bioassay was conducted in 500 mL glass bottles, each was tilled with thirty snails and 450 mL of water. The concentration of test compounds ranged from 0.5 to 250 mg/L (6 different concentrations, three replications). After 72 hrs exposure, the number of dead snails were recorded. The snail was considered to be dead if there was no response to touch, the operculum shut tight, but with no resistance when the operculum was lifted, and the foot was out and swollen.The lethal effect concentration ( LC50) was determined to be 16.9 mg/l(linear regression (r2)= 2.441x + 8.742(0.942)). Based on the above effect concentration it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.
2)Short term toxicity to aquatic invertebrate Pomacea canaliculata (golden apple snail) was evaluated for the test material. Aquaria with dimensions of 50 cm x 25 cm x 35 cm were used for maintaining and breeding snails in the laboratory. The top of the aquarium was covered with nylon screen. Lamps were positioned 15 cm above the aquaria with a cycle of 16 hrs “on”, and 8 hrs “off’. The aquarium floor was covered with a layer of small pebbles, and was filled with water to a depth of 25 cm. Tap water reduced the thickness of the snail shell and the number of eggs, dechlorinated tap water caused the snails to secrete mucus. As a result, fresh aerated well water (pH 7.5, 26 °C) was provided in a continuous flow. Wild adult snails, with a diameter greater than 5 cm, were collected from the field. After copulation, fresh eggs were incubated and the young snails were hatched in 10-17 days. The young snails were fed sweet potato leaves in the aquarium for 65-70 days until maturity. The breeding was repeated and the third generation snails of 35-40 days old were used for toxicity testing.
The bioassay was conducted in 500 mL glass bottles, each was tilled with thirty snails and 450 mL of water. The concentration of test compounds ranged from 0.5 to 250 mg/L (6 different concentrations, three replications). After 72 hrs exposure, the number of dead snails were recorded. The snail was considered to be dead if there was no response to touch, the operculum shut tight, but with no resistance when the operculum was lifted, and the foot was out and swollen. The lethal effect concentration ( LC50) was determined to be 67.4 mg/l linear regression (r2)= 0.617 x + 8.382(0.932). Based on the above effect concentration it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.
3) Short term toxicity test for aquatic invertebrtae was performed for the test material for 24 h . The EC50 value was observed to be 52.6 mg/l , The EC0 was noted at 31.2 mg/l and the EC100 was observed in the concentration 125 mg/l.Based on the above effect concentartions it can be considered that test material was toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.
Based on the above effect concentartions it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.
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