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EC number: 204-493-5 | CAS number: 121-69-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
The Salmonella Mutagenicity Test or Ames Test for N,N-dimethylaniline found to be negative. The highest ineffective dose tested in any Salmonella typhimurium strain was 333 µg/plate.
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Qualifier:
- according to guideline
- Guideline:
- other:
- Principles of method if other than guideline:
- The Salmonella Ames Test: Preincubation method
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
- Target gene:
- histidine-manufacturing gene
- Species / strain / cell type:
- S. typhimurium TA 100
- Additional strain / cell type characteristics:
- other: histidine deficient strain
- Species / strain / cell type:
- S. typhimurium TA 1535
- Additional strain / cell type characteristics:
- other: histidine deficient strain
- Metabolic activation:
- with and without
- Metabolic activation system:
- 10% HLI/RLI
- Test concentrations with justification for top dose:
- 3, 10, 33, 100, 333, 1000 µg/Plate
- Vehicle / solvent:
- Dimethyl Sulfoxide
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Dimethyl Sulfoxide
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- in the absence of S9 activation
- Positive control substance:
- sodium azide
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Dimethyl Sulfoxide
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- in the presence of S9 activation by 10% HLI/RLI
- Positive control substance:
- other: 2-aminoanthracene
- Details on test system and experimental conditions:
- RLI = induced male Sprague Dawley rat liver S9
HLI = induced male Syrian hamster liver S9 - Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
- Conclusions:
- Interpretation of results (migrated information):
negative
N,N-dimethylaniline gives negative result for genetic toxicity in Ames test conducted on to S. typhimurium TA 100 and TA1535 with and without metabolic activation by 10% HLI/RLI S9 system. - Executive summary:
N,N-dimethylaniline gives negative result for genetic toxicity in Ames test conducted on to S. typhimurium TA 100 and TA1535 with and without metabolic activation by 10% HLI/RLI S9 system.
Reference
Strain: TA100
Dose |
No Activation |
No Activation |
10% HLI |
10% HLI |
10% RLI |
10% RLI |
||||||
Protocol |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
||||||
ug/Plate |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
85 |
2.7 |
93 |
3.7 |
123 |
10 |
87 |
3.1 |
97 |
2.2 |
94 |
9 |
3 |
107 |
3.2 |
107 |
8.5 |
|
|
104 |
4.7 |
|
|
108 |
11.7 |
10 |
89 |
5.6 |
102 |
8 |
112 |
8.7 |
89 |
6 |
110 |
7.9 |
108 |
4.8 |
33 |
104 |
11.3 |
97 |
10.3 |
113 |
1.8 |
104 |
3 |
110 |
6.2 |
101 |
6.2 |
100 |
109 |
12.5 |
95 |
9.9 |
102 |
9.3 |
87 |
3.3 |
113 |
4.5 |
105 |
2.6 |
333 |
102 |
3.5 |
81 |
3.7 |
122 |
12.7 |
93 |
2.7 |
119 |
11.1 |
102 |
8.1 |
1000 |
|
|
|
|
40s |
9.3 |
|
|
78s |
13.4 |
|
|
Positive Control |
382 |
7.7 |
345 |
17.7 |
1336 |
56.6 |
1132 |
40.3 |
461 |
26.6 |
441 |
9.1 |
Strain: TA1535
Dose |
No Activation |
No Activation |
10% HLI |
10% HLI |
10% RLI |
10% RLI |
||||||
Protocol |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
||||||
ug/Plate |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
23 |
1.9 |
28 |
3.7 |
9 |
1.9 |
6 |
0.3 |
8 |
0.6 |
10 |
1.2 |
3 |
14 |
0.9 |
20 |
3.8 |
|
|
7 |
1 |
|
|
9 |
0.3 |
10 |
14 |
3.2 |
21 |
2.2 |
10 |
1.2 |
7 |
2.8 |
7 |
0.7 |
8 |
2.2 |
33 |
16 |
1.5 |
18 |
1.9 |
8 |
0.3 |
4 |
1.2 |
6 |
0 |
7 |
0.7 |
100 |
15 |
0.3 |
18 |
2.8 |
6 |
0.3 |
7 |
0.6 |
7 |
0.9 |
6 |
1 |
333 |
15 |
2.1 |
20 |
2.9 |
7 |
1.2 |
6 |
1 |
6 |
1 |
5 |
0.9 |
1000 |
|
|
|
|
2s |
1 |
|
|
3s |
1.2 |
|
|
Positive Control |
519 |
6.8 |
324 |
21.2 |
438 |
43 |
452 |
9.8 |
158 |
11.5 |
187 |
9.9 |
s = Slight Toxicity; p = Precipitate; x = Slight Toxicity and Precipitate; T = Toxic; c = Contamination
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Additional information from genetic toxicity in vitro:
Ames test:
The Salmonella Mutagenicity Test or Ames Test for N,N-dimethylaniline found to be negative in Salmonella typhimurium strains (TA98, TA1535 and TA100) in the presence and absence of rat and hamster liver S-9, at doses of 3, 10, 33, 100, 333 and 1000 µg/plate.
N,N-dimethylaniline was found to be negative in the Salmonella/mammalian microsome assay conducted on Salmonella typhimurium TA98, TA100, TA1537, TA1538 with and without metabolic activation by rat and hamster liver S-9 system.
Chromosome aberration:
N,N-dimethylaniline gives positive result in Chromosome Aberration test conducted on Chinese hamster Ovary (CHO) cells with and without metabolic activation by Induced Rat Liver S9 system.
N,N-dimethylaniline did not show any mutagenic activity on primary rat hepatocytes in an in vitro DNA repair test.
Justification for selection of genetic toxicity endpoint
N,N-dimethylaniline was found to be negative when tested for mutagenicity in the Salmonella/microsome preincubation assay using the standard protocol approved by the National Toxicology Program (NTP). N,N-dimethylaniline was tested in Salmonella typhimurium strains (TA1535 and TA100) in the presence and absence of rat and hamster liver S-9, at doses of 3, 10, 33, 100, 333 and 1000 µg/plate. The highest ineffective dose tested in any Salmonella typhimurium strain was 333 µg/plate.
Justification for classification or non-classification
The studies reviewed indicate that the test substance N,N-dimethylaniline is non genetoxic
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