Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 435-940-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The actual concentrations of the dissolved ions of the test substance were determined in the surplus of the test media before the test and in the pooled test media of the replicates after 48 hours.
Sample preparation: To 9.5 ml sample 0.5 ml nitric acid (suprapur) were added.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A preparation with 100 mg/L loading rate was prepared by adding 50 mg test substance to 500 mL dilution water. This suspension was stirred for 24 hours in the dark by using magnetic stir plates and bars. A filtrate (two times filtration by a folded filter and once by a cellulose-acetate filter 0.2 µm) was then obtained to ensure that no undissolved test substance particles were in the medium.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Neonates of Daphnia magna less than 24 hours old, were obtained by hatching ephippia, from Creasel Co., Fazantenpark 9, 98000 Deinze, Belgium..
The ephippia were hatched in a petri dish containing reconstituted water in a temperature controlled room at approx. 20 °C under continuous illumination of approximately 6 000 Lux. According to Creasel hatching occurs between 72 and 96 hours after initiating hatching. Approximately 2 hours before the start of the test the neonates were fed with a suspension of Spirulina powder, as also in traditional cultures the neonates have the opportunity to take up some feed particles. The neonates were collected for this test at approximately 87 hours after initiation of hatching.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No.
Test conditions
- Hardness:
- 250 mg CaCO3/L.
- Test temperature:
- 20 °C.
- pH:
- 7.3 to 7.4 in the test substance media.
- Dissolved oxygen:
- At least 7.6 mg/L.
- Salinity:
- No.
- Nominal and measured concentrations:
- Nominal: Loading rate of 100 mg test substance per L water.
Measured, based on ions. The sum of the ions was calculated as an indicator of an upper limit of the dissolved test substance. - Details on test conditions:
- Test vessels: Disposable multiwell test plates with 30 test wells with a transparent lid, provided with the testkit. Each plate has four wells each (replicates A,B,C,D) for the control and for the test concentration. Additionally, for each concentration the plates are provided with "rinsing wells" to prevent dilution of the test substance during the transfer of the daphnia from the hatching petri dish to the test wells (see below).
Water: Reconstituted water.
Aeration: No extra aeration, but slight circulation of air is still possible when the lid of the test plate is closed.
Temperature: Ca. 21 °C, monitored daily (temperature controlled room).
Light: Artificial light from 4 a.m. to 8 p.m.
Feed: No feed.
Transfer to the test wells: The "rinsing wells" and the wells for the replicates of each concentration and control respectively were filled with 10 mL medium each. For the test substance concentration and for the control at least 20 actively swimming neonates were transferred with a micropipette from the hatching petri dish to the "rinsing well". Then 5 neonates from the "rinsing wells" were transferred to each of the 4 wells of the appropriate concentration. - Reference substance (positive control):
- yes
- Remarks:
- periodic check with potassium dichromate.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 other: mg loading / L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 other: mg loading / L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 100 other: mg loading / L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: constitutive elements
- Basis for effect:
- mobility
- Remarks on result:
- other: The EC50 is higher than the water solubility
Any other information on results incl. tables
Actual test substance concentrations
The solubility of the test substance was non-stoichiometric. The solubility of lithium and phosphate was much higher than that of the other components. This indicates that the substance does not dissolve as one substance but hydrolyses with releasing some elements to a greater extent than others. The sum of the dissolved ions of the substance was 4.33 mg/L before the test and 4.27 mg/L after the test.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48 h toxicity (EC50) of the substance to Daphnia is higher than the loading rate of 100 mg/L and higher than the water solubility of the substance.
- Executive summary:
The acute toxicity of the substance to Daphnia magna was determined in a static test according to the EU method C.2. The solubility of the substance in water is very low and therefore an aqueous extract of 100 mg/L loading rate, obtained by filtration, was tested. The dissolved ions of the test substance were determined by ICP/AES-technique.
Results
- Immobilisation of control group and test group daphnids was 0 % at the end of the test.
- There were no daphnids trapped at the water surface.
- Temperature was stable during the test period.
- Dissolved oxygen concentrations in the test vessels were not lower than 7.6 mg/L at each time determined.
- The amount of the dissolved ions was similar at the start and at the end of the test.
The 48 h toxicity (EC50) of the substance to Daphnia is higher than the loading rate of 100 mg/L and higher than the water solubility of the substance.
The sum of the measured dissolved constitutive ions of the substance was 4.3 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.