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EC number: 236-655-6 | CAS number: 13455-36-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 10, 2022 - May 31, 2022
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 022
- Report date:
- 2022
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- adopted 21 July 1997, corrected 26 June 2020
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Tricobalt bis(orthophosphate)
- EC Number:
- 236-655-6
- EC Name:
- Tricobalt bis(orthophosphate)
- Cas Number:
- 13455-36-2
- Molecular formula:
- Co3(PO4)2
- IUPAC Name:
- tricobalt bis(orthophosphate)
- Reference substance name:
- Dicobalt diphosphate
- EC Number:
- 238-689-7
- EC Name:
- Dicobalt diphosphate
- Cas Number:
- 14640-56-3
- Molecular formula:
- Co2P2O7
- IUPAC Name:
- dicobalt diphosphate
- Reference substance name:
- cobalt phosphate octahydrate
- Cas Number:
- 10294-50-5
- Molecular formula:
- Co3(PO4)2.8H2O
- IUPAC Name:
- cobalt phosphate octahydrate
- Test material form:
- solid: particulate/powder
Constituent 1
impurity 1
impurity 2
Method
- Target gene:
- Not applicable
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- metabolic activation by a microsomal fraction of rat liver (S9-mix 10%v/v)
- Test concentrations with justification for top dose:
- - Concentrations for assay n°1: 50, 150, 500, 1500 and 5000 µg/plate
- Concentrations for assay n°2: 50, 150, 500, 1500 and 5000 µg/plate - Vehicle / solvent:
- Solutions were prepared in ethanol.
Controlsopen allclose all
- Untreated negative controls:
- yes
- True negative controls:
- yes
- Remarks:
- Absolute negative control containing no test item corresponding to the spontaneous reversion rate
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Ethanol
- Remarks:
- Vehicle used to solubilize test item. Test item was completely soluble in ethanol at all concentrations tested.
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Remarks:
- Solvent used to solubilize positive controls
- Negative solvent / vehicle controls:
- yes
- Remarks:
- NaCl 0.15 M
- Remarks:
- Solvent used to solubilize positive controls
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- Stock solution was formulated in DMSO.
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- Stock solution was formulated in NaCl 0.15 M.
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Stock solution was formulated in DMSO.
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Remarks:
- Stock solution was formulated in NaCl 0.15M.
- Positive controls:
- yes
- Positive control substance:
- other: 2-anthramine: for TA1535, TA1537, TA98, TA100 and E. Coli with S9
- Remarks:
- Stock solution was formulated in DMSO.
- Details on test system and experimental conditions:
- PREPARATION OF STOCK SOLUTION
-Test item is completely soluble in ethanol at all concentrations tested.
- Stock solution for each assay was prepared extemporaneously at 100 mg/mL in ethanol.
- Test item is tested at 5000, 1500, 500, 150 and 50 µg/plate.
- Samples were taken under constant stirring.
BACTERIA
- Strains of Salmonella typhimurium and Eschericia coli are purchased from Moltox. They are maintained in laboratory.
- The genotype of bacterial strains was checked.
EXPERIMENTS
- Triplicate plates test material.
- Negative, positive and vehicle controls were included in triplicate with and without metabolic activation.
- S9 fraction, microsome fraction, prepared from Sprague Dawley rat liver homogenate, was provided by Moltox USA (S9 Moltox-11-101.5-4477 validated on 11.10.2021 - expiry date: 13.07.2023).
- For experiments without metabolic activiation: 0.1 mL bacterial culture, 50 µL each dilution of the originial solution or control and 0.5 mL of phosphate buffer were added to 2 mL overlay agar maintained super cooled in 45°C containing 10%v/v of a L-Histidine-D-Biotine solution (0.5 mM) for Salmonella Typhimurium strains or 5%v/v of nutrient broth n°2 to which are added 5 µL of a L-Tryptophane solution at 2 mg/L for Escherichia coli strain. Plates are incubated at 37°C over 48-72 hour period.The pre-incubation phase is performed for the second assay if the first assay is negative.
- For experiments with metabolic activation: protocol similar to that described above, except that 500 µL of S9-mix fraction is quickly added before pouring the mixture onto the plates. Since the first assay was negative, the pre-incubation test was performed for the second assay.
COLONY COUNTING
- After a 48-72 hour incubation period at 37°C, revertant colonies are counted in each plate. - Evaluation criteria:
- The following validity criteria were checked to validate each experiment:
- the bacteriostatic activity of the highest concentration tested shall be equal to or less than 75%,
- the spontaneous reversion rate of the absolute negative control shall comply with the historical values of the laboratory,
- in presence of the solvent the spontaneous reversion rate shall comply with the historical values of the absolute negative control of the laboratory,
- the mean number of revertant colonies obtained for each strain and the corresponding positive control with and/or without metabolic activation shall comply with the historical values of the laboratory,
- negative and positive values should not show significant difference with the historical values of the laboratory (+/- 2 standard deviations).
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- not valid
- Additional information on results:
- There is no difference between the number of spontaneous reversions, the number of reversions obtained for positive controls (with and without metabolic activation), and the mean of corresponding experimental historical values obtained in the laboratory.
There is no evidence of any increase in the number of revertant colonies in the presence of the test item without and with metabolic activaiton in Salmonella typhimurium TA1535, TA1537, TA98, TA100 strains and Escherichia coli WP2(uvrA)(pKM101) strain.
Results are confirmed in an independant experiment.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative
In the assay conditions, solutions prepared from test item do not induce any mutagenic change in Salmonella typhimurium TA1535, TA1537, TA98, TA100 and Eschirichia coli WP2(urA-)(pKM101) without, or with metabolic activation, according to the OECD guidelines n°471.
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