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EC number: 620-365-5 | CAS number: 9016-72-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009/12/07 to 2009/12/17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- To measure the oxygen consumption, 250 mL of sludge with test item (or control or reference compound) was incubated for 3 h in 300 mL closed Erlenmeyer flasks (with air inlet and outlet) and aerated through a glass tube at 50-100 L/h with clean oil-free air. For measurement, the content of the Erlenmeyer flasks was completely transferred to 250 mL BOD bottles and O2-content was measured with an O2-meter (redox electrode) with writer.
The respiration rate for each concentration was determined graphically from the linear part of the curve of O2-content versus time. The inhibitory effect of the test item at a particular concentration is expressed as a percentage of the mean of the respiration rates of two controls. An EC50 value was calculated from the respiration rates at different test item concentrations. - Details on test solutions:
- 10 mL of sludge suspension were dried in order to calculate the amount of sludge suspension to achieve a concentration of activated sludge of 2 g/L (dry weight) suspended solids. The calculated amount of sludge suspension was taken and filled up to a defined end volume with deionised water.
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Type : mixed population of aquatic microorganisms (activated sludge)
Origin : aeration tank of a domestic sewage treatment plant - Test type:
- static
- Water media type:
- other: deionised water
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 19.4 - 19.8°C
- pH:
- 7.9 - 8.0
- Dissolved oxygen:
- 2.5 - 6.4 mg O2/L
- Nominal and measured concentrations:
- Nominal concentrations: 100, 180, 320, 560 and 1000 mg a.s./L
- Details on test conditions:
- The respiration rate of each mixture was determined after aeration periods of 3 hours. Two controls without test item were included in the test design, one at the start and the other at the end of the test series.
The pH was measured and adjusted to pH 6-8. 8 mL of the synthetic medium and 100 mL of activated sludge were added to the dissolved test item. The mixture was filled up with deionised water to 250 mL and aerated at 20°C ± 2°C. The exposure medium with the reference substance was prepared by adding 8 mL of the synthetic medium, 100 mL of activated sludge and a defined amount of the stock solution to achieve the test concentrations and was filled up to 250 mL with deionised water. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 404.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Propineb showed 17.8 % respiration inhibition of activated sludge at a test item concentration of 1000 mg a.s./L.
- Results with reference substance (positive control):
- The EC50 of the reference compound 3,5-Dichlorophenol is in the range 5 – 30 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The activated sludge was exposed to propineb at nominal concentrations of 100, 180, 320, 560 and 1000 mg a.s./L. The respiration rate of each mixture was determined after aeration periods of 3 hours. All validity criteria of the test method were met. The EC50 was determined to be >1000 mg a.s./L. Propineb showed 17.8% respiration inhibition of activated sludge at the highest test concentration of 1000 mg a.s./L.
- Executive summary:
A study was performed to assess the toxicity of Propineb to bacteria. The study was conducted in accordance with Council Regulation (EC) No 440/2008, Method C.11 ”Activated sludge respiration inhibition” (2008). This test method is equal to OECD Guideline 209 (1984). The activated sludge was exposed to propineb atnominal concentrations of 100, 180, 320, 560 and 1000 mg a.s./L. The respiration rate of each mixture was determined after aeration periods of 3 hours. Two controls without test item were included in the test design, one at the start and the other at the end of the test series. Propineb showed 17.8 % respiration inhibition of activated sludge at the highest test concentration of 1000 mg a.s./L. The EC50 was determined to be >1000 mg a.s./L.
Reference
Table 1
Oxygen content, temperature and pH-values during exposure phase (test item)
Test item concentration [mg/L] | O2 start [mg O2/L] | O2 end [mg O2/L] | Time (startend) [min.] | Temp. [°C] | pH | |
Test item | 100 | 5.6 | 2.7 | 5 | 19.6 | 7.9 |
180 | 5.8 | 2.5 | 6 | 19.6 | 7.9 | |
320 | 6.4 | 2.7 | 7 | 19.8 | 8.0 | |
560 | 6.5 | 2.7 | 8 | 19.7 | 8.0 | |
1000 | 6.2 | 2.9 | 7 | 19.7 | 7.9 | |
control 1 | 6.1 | 3.2 | 5 | 19.4 | 7.9 | |
control 2 | 6.4 | 3.0 | 6 | 19.5 | 7.9 | |
Physicochemical oxygen consumption control * | 8.8 | 8.8 | 8 | 19.1 | 7.3 |
Comments: * Data obtained in the range finding test.
The test item was added to about 130 mL deionized water and stirred overnight before testing (equilibration phase).
Start of stirring period of the test item: 3 p.m., 2009-12-09
End of stirring period of the test item: 7 a.m., 2009-12-10
Table 2
Results of test item Propineb
Test item [mg/L] | Respiratory rate [mg/L x h) | Phys.-chem. [mg/L x h] | Respiratory rate [mg/L x h] | Inhibition [%] |
100 | 34.8 | 0.0 | 34.8 | 0.0 |
180 | 33.0 | 0.0 | 33.0 | 4.1 |
320 | 31.7 | 0.0 | 31.7 | 7.8 |
560 | 28.5 | 0.0 | 28.5 | 17.2 |
1000* | 28.3 | 0.0 | 28.3 | 17.8 |
Control mean | 34.4 | - | - | - |
Control 1 | 34.8 | - | - | - |
Control 2 | 34.0 | - | - | - |
Comments: Test concentrations are given as nominal concentrations as they were not confirmed by analytical methods.
* The physico-chemical oxygen consumption has been determined at 1000 mg/L test item concentration. As no physico-chemical oxygen consumption was observed at that test item concentration this observation also holds true for the lower test item concentrations.
Description of key information
The activated sludge was exposed to propineb at nominal concentrations of 100, 180, 320, 560 and 1000 mg a.s./L. The respiration rate of each mixture was determined after aeration periods of 3 hours. All validity criteria of the test method were met. The EC50 was determined to be >1000 mg a.s./L. Propineb showed 17.8% respiration inhibition of activated sludge at the highest test concentration of 1000 mg a.s./L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 404.4 mg/L
Additional information
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