Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 236-291-8 | CAS number: 13282-70-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014-11-10 to 2015-03-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Nanjing Chengdong Sewage Treatment Plant, Batch No.: AS20141202.
- Laboratory culture: No
- Storage conditions: NS
- Storage length: NS
- Preparation of inoculum for exposure: The sludge was washed three times by Basal Salts Medium and centrifugation
- Concentration of sludge: Mixed Liquor suspended solids levels of 4g
- Water filtered: yes
- Type and size of filter used, if any:Water Purification System (mili-Q, Millipore,USA) - Duration of test (contact time):
- 28 d
- Initial conc.:
- 29.9 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The Basal salts Medium used for the experiment was prepared by mixing 10 mL of solution A with 800 mL deionised water, followed by addition of 1 mL of solutions B, C and D, then the appropriate amount of deionised water was added to make up 1 L. Solutions A, B and C are as follows:
Stock solution A:
KH2PO4 (potassium dihydrogen phosphate): 8.5 g/L
K2HPO4 (dipotassium hydrogen phosphate): 21.75 g/L
Na2HPO4.2H2O (disodium monohydrogen phosphate dehydrate): 33.4 g/L
NH4Cl (Ammonium chloride): 0.5 g/L
Dissolved and made up to 1 L with deionised water
pH: 7.42
Stock solution B
CaCl2 (Calcium chloride): 27.5 g/L
Dissolved and made up to 1 L with deionised water
Stock solution C
MgSO4.7H2O (Magnesium sulphate heptahydrate): 22.5 g/L
Dissolved and made up to 1 L with deionised water
Stock solution D
FeCl3.6H2O (Iron(III)chloride hexahydrate): 0.25 g/L
Dissolved and made up to 1 L with deionised water
Added one drop of concentrated hydrochloric acid.
- Additional substrate: NA
- Test temperature: 21.5 - 22.1 degrees Celsius
- pH: 7.31 - 7.68
- pH adjusted: NS
- CEC (meq/100 g):
- Aeration of dilution water: NS
- Suspended solids concentration: 30 g/L (in vessel)
- Continuous darkness: yes
- Other:
TEST SYSTEM
- Culturing apparatus: Flasks (BOD bottle vessel flasks, total volume: 500 mL, volume of contents: 300 mL each) with Teflon magnetic stirrers. Air pump (AC0 -318, Guangdong Haley Group Co., Ltd.)
- Number of culture flasks/concentration: 2 test flasks (test substance 29.9 mg/L starting concentration, 30 mg/L inoculum), 2 inoculum (30 mg/L) controls, 1 procedural control (with reference substance (50 mg/L) and inoculum (30 mg/L)), 1 inhibition control (test substance (29.9 mg/L, reference substance (50 mg/L) and inoculum (30 mg/L)).
- Method used to create aerobic conditions: NA
- Method used to create anaerobic conditions: NS
- Measuring equipment: BOD meter (OxiTop Control 6, WTW, Germany)
SAMPLING
- Sampling frequency: daily
- Sampling method: Oxygen uptake measured from the apparatus - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- test concentration 50 mg/L, Lot No.: 20130117, purity ≥99.5%
- Test performance:
- Test ran according to plan
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 97
- Sampling time:
- 28 d
- Details on results:
- The test temperature remained between 21.5 and 22.1 degrees Celsius, measured pH remained between 7.31 and 7.68. Total oxygen uptake by the inoculum blank was 29 mg O2/L at the end of the test (therefore fulfilled the validity criteria of not exceeding 60 mg O2/L). The differences between replicate values for inoculum blank and test were less than 20%. Biodegradation of inhibition control was 80% at 14 days (above the minimum of 25%), therefore there was no inhibition effect to the inoculum.
The results showed that under the specific experimental conditions, biodegradation of the test substance calculated based on ThOD (NO3) was 97% after 28 days and 75.7% during at the 10 day window measurements. (See Biodegradation curve figure in the "Overall remarks, attachments" section) According to they experiment the test substance can therefore be classed as readily biodegradable. - Results with reference substance:
- The biodegradation percentage of the reference substance, sodium benzoate, was 68.3% at day 14, confirming the suitability of the inoculum used (above the minimum of 25% stated by guideline).
- Validity criteria fulfilled:
- yes
- Remarks:
- See details in the "Results and Discussion" section
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The results showed that under the specific experimental conditions, biodegradation of the test substance calculated based on ThOD (NO3) was 97%. As the 10-day window was achieved, the substance is considered readily biodegradable.
- Executive summary:
The study was performed to assess the biotic degradation of the test substance by performing a ready biodegradability test, according to OECD Test Guideline 301F and EU Method C.4, with GLP compliance.
Test vessels were filled with the test substance at a nominal concentration of 29.9 mg/L and inoculated with activated sludge at a concentration of suspended solids of 30 mg/L. In the meantime, a series of blanks were filled with inoculated mineral medium. Test vessels containing the reference substance sodium benzoate ( 50 mg/L) were tested in order to check the procedure. A toxicity control, containing both the test substance and the reference substance, was also performed in order to check the absence of test substance toxic effect on the microbial inoculum. The oxygen uptake in test vessels from each group incubated at temperatures between 21.5°C and 22.1°C in darkness was continuously recorded until the end of the test.
All validity criteria were fulfilled. The reference substance, sodium benzoate, was biodegraded by 68.3 % after 14 days and the toxicity control showed that the test substance had no inhibitory effect on the activity of the microbial inoculum.
In conclusion, the results showed that under the specific experimental conditions, biodegradation of the test substance calculated based on ThOD (NO3) was 97% after 28 days. As the 10-day window was achieved, the test substance can be considered as readily biodegradable under the experimental conditions.
Reference
Description of key information
Ready biodegradable; OECD 301F; Linjun, Z. (2015)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
The key study was performed to assess the biotic degradation of the test substance by performing a ready biodegradability test, according to OECD Test Guideline 301F and EU Method C.4, with GLP compliance.
Test vessels were filled with the test substance at a nominal concentration of 29.9 mg/L and inoculated with activated sludge at a concentration of suspended solids of 30 mg/L. In the meantime, a series of blanks were filled with inoculated mineral medium. Test vessels containing the reference substance sodium benzoate ( 50 mg/L) were tested in order to check the procedure. A toxicity control, containing both the test substance and the reference substance, was also performed in order to check the absence of test substance toxic effect on the microbial inoculum. The oxygen uptake in test vessels from each group incubated at temperatures between 21.5°C and 22.1°C in darkness was continuously recorded until the end of the test.
All validity criteria were fulfilled. The reference substance, sodium benzoate, was biodegraded by 68.3 % after 14 days and the toxicity control showed that the test substance had no inhibitory effect on the activity of the microbial inoculum.
In conclusion, the results showed that under the specific experimental conditions, biodegradation of the test substance calculated based on ThOD (NO3) was 97% after 28 days. As the 10-day window was achieved, the test substance can be considered as readily biodegradable under the experimental conditions.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.