4,4-dimethyl-3,5,8-trioxabicyclo[5.1.0]octane

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Jul 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

(adopted 1993)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Remarks:

GC/MSD

Details on sampling:

- Sampling method: Samples for analytical monitoring were taken at 0 and 96 hours of exposure. Aliquots of 1 mL were diluted with methanol (1:10) and injected to GC/MSD (injection volume 2 µL).

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: to produce the test concentration 2.00 g of the test substance were dissolved in 10 L tap water in the test aquaria. This solution was stirred by hand.
- Controls: 1 control

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: German bred; distributor: Westaquarium, Zierfischzüchterei Bad Lauterberg
- Length at study initiation (length definition, mean, range and SD): 2.5 cm +/- 0.5 cm (estimated)

ACCLIMATION
- Acclimation period: The fish were acclimatized for 17 days before start of the test in tap water
- Feeding frequency during acclimation: feed twice daily
- Health during acclimation (any mortality observed): no mortality was observed during acclimatization period.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

20.5 to 21.3 °C

pH:

7.5 to 8.1

Dissolved oxygen:

7.3 to 9.2 mg/L (=81 to 104 % of air saturation)

Nominal and measured concentrations:

100 mg/L (nominal concentration)

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria
- Material, size, headspace, fill volume: 25 L
- Aeration: test and control solution were not aerated during exposure

- No. of organisms per vessel: 10 fish, randomly distributed into subgroups of 5
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: normal tap water was used as dilution water, not polluted with harmful concentrations of chlorine, heavy metals or other substances, based on quarterly measurements.
- Culture medium different from test medium: no
- Intervals of water quality measurement: oxygen, pH-value and temperature were recorded at 3, 24, 48, 72 and 96 h of exposure.

OTHER TEST CONDITIONS
- Photoperiod: The light/dark rhythm was 12 hours/ 12 hours.


EFFECT PARAMETERS MEASURED
- Mortalities and visual abnormalities were recorded at approximately 3, 6, 24, 48, 72 and 96 hours of exposure.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: limit test with 100 mg/L (nominal concentration).

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of test concentration: No mortality was observed in the test concentration
- Mortality of control: No mortality was observed in the control
- Abnormal responses: No fish showed any abnormal behavior

Sublethal observations / clinical signs:

Concentration analysis of the test substance and control

Nominal concentration [mg/L]	0 h	96 h	Mean value
0	0	0	0
100	101.6	79.3	90.5

Validity criteria for the measurement of the fish toxicity

Target condition according to guideline:	Actual condition according to the study:	Validity criteria met:
In the control(s) (dilution water control, solvent control), the mortality should not exceed 10% (or one fish, if fewer than 10 control fish are tested) at the end of the exposure.	Mortality of control: 0 fish died	Yes
The dissolved oxygen concentration should be >/= 60% of the air saturation value in all test vessels throughout the exposure period.	The oxygen saturation was measured in a 24 hour interval and ranged between 81% to 104% of air saturation.	Yes
There should be evidence that the concentration of the test substance has been satisfactorily maintained, and preferably it should be at least 80% of the nominal concentration throughout the exposure.	Recovery rates correspond to 101.6% of nominal value at 0 hours and to 79.3% of nominal value at 96 hours. At 96 hours of exposure, the test concentration fall slightly below the preferred 80%, however, as deviations accounted only to 0.7%, this should not have had any influence on the test results.	Yes
The water temperature should not differ by more than 1.5°C between test vessels or between successive days at any time during the exposure. Temperature should be appropriate to the specified test species, e.g., for zebrafish within the range of 21-25°C and should not vary more than ± 1°C within the recommended range.	During the test, a temperature range of 20.5 to 21.3°C was maintained in the test vessels. The temperature in the test aquaria was occasionally slightly below the range recommended for zebra fish. However, as temperature were only 0.5°C below the reccomended range and did not vary by more than 1°C this deviation should not have had any influence on the test results.	Yes
The pH should not vary by more than one unit	During the test, the measured pH values in the test vessels were in the range of 7.5 to 8.1.	Yes
A limit test may be performed at 100 mg/L of the test substance in order to demonstrate that the EC50 is greater than this concentration. The limit test should be performed using at least 7 fish, with the same number in the control. If any mortalities occur, a full study should be conducted.	Within the test, 10 fish were used for the test concentration (100 mg/L) and 10 for the control, no mortalities occured.	Yes

 

Validity criteria fulfilled:

yes

Remarks:

See 'Any other information on results incl. tables'.

Conclusions:

Results of this study showed the LC50 (96h) >/= 100 mg/L (nominal concentration) and demonstrate that the test substance is not acutely toxic to fish up to 100 mg/L (nominal concentration).

Executive summary:

The study was performed to assess the acute toxicity of the test substance to zebra fish (Brachydanio rerio) under static conditions. The study was conducted in accordance with the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test'. Groups of ten fish were exposed to a limit test concentration of nominally 100 mg/L of the test substance dissolved in tap water. Mortalities and visual abnormalities were recorder after 3, 6, 24, 48, 72, and 96 hours of exposure. The following values were determined: LC50 (96h) >/=100 mg/L nominal concentration of the test substance. Recovery rates correspond to 101.6% of nominal value at 0 hours and to 79.3% of nominal value at 96 hours. No toxix effects against fish at 100 mg/L (nominal concentration) were observed.

Description of key information

A study was performed to assess the acute toxicity of the test substance to zebra fish (Brachydanio rerio) under static conditions. The study was conducted in accordance with the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test'. Groups of ten fish were exposed to a limit test concentration of nominally 100 mg/L of the test substance dissolved in tap water. Mortalities and visual abnormalities were recorder after 3, 6, 24, 48, 72, and 96 hours of exposure. The following values were determined: LC50 (96h) >/=100 mg/L nominal concentration of the test substance. Recovery rates correspond to 101.6% of nominal value at 0 hours and to 79.3% of nominal value at 96 hours. No toxic effects against fish at 100 mg/L (nominal concentration) were observed.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

>= 100 mg/L