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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2018-04-12 to 2018-08-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Cobalt, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, sulfonated, sodium salts
- EC Number:
- 290-971-9
- EC Name:
- Cobalt, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, sulfonated, sodium salts
- Cas Number:
- 90294-83-0
- Molecular formula:
- CoC32H16-τN8(SO3Na)τ
- IUPAC Name:
- Cobalt, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, sulfonated, sodium salts
- Test material form:
- solid: particulate/powder
1
- Specific details on test material used for the study:
- STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature, protected from light, original container
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
* 0 mg/L of test item (control)
* 100 mg/L of test item
- Sampling method: sampling done at the start of the exposure (0 hours) of the fresh media (control and limit concentration) after preparation of the limit concentration and at the end of the exposure (48 hours) of the old media (control and limit concentration), directly from the test vessels.
- Sample storage conditions before analysis: at 6 +/- 2°C until the start of the analysis, if necessary
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A limit concentration (100 mg/L of the test item were weighed out) was freshly prepared with dilution water prior to the start of the exposure (at 0 hours). The test solution was agitated until it was visually clear.
- Controls: dilution water without test item, incubated under the same conditions as the test groups).
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnid
- Strain/clone: STRAUSS
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), 14195 Berlin, Germany
- Age of parental stock (mean and range, SD): less than 24 hours (at test initiation)
- Feeding during test: no
- Acclimation period: No acclimatation period as the daphnids were cultured under the same conditions as the test replicates.
Study design
- Test type:
- static
- Water media type:
- other: medium Elendt M4 according to OECD 202, Annex 3 (2004)
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 271 mg CaCo3/L (8 hours, in dilution water)
- Test temperature:
- 18 - 22°C ± 1°C
- pH:
- Fresh media at start of exposure: 7.55 - 7.62 (100 mg/L - control)
Dilution water at start of exposure: 7.62 - Dissolved oxygen:
- Fresh media at start of exposure: 8.89 mg/L - 9.33 mg/L (100 mg/L - control)
Dilution water at start of exposure: 9.33 mg/L
Old media at end of exposure: .8.33 mg/L - 8.76 mg/L (100 mg/L - control) - Nominal and measured concentrations:
- Nominal concentration : 100 mg/L of test item
Measured concentrations at start of exposure: 96.2 %
Measured concentration at end of exposure: 90.6% - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (4 (ID) x 7 (H) cm), 50 mL capacity
- Type : closed with watch glasses
- Material, size, headspace, fill volume: 50 mL glass beaker, filled at 20 mL
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): no renewal
- No. of organisms per vessel: 5 daphnids
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 4 replicates
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light, 8 hours darkness
- Light intensity: Diffuse light, maximum of 1500 lx
RANGE-FINDING STUDY
- Test concentrations:
* 0 mg/l of test item (control)
* 1.00 mg/l of test item
* 10.0 mg/l of test item
* 100 mg/l of test item
- Results used to determine the conditions for the definitive study: Immobilization rates were used to determine the conditions for the definitive study - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (K2Cr2O7)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: non observed
- Mortality of control: No mortality observed - Results with reference substance (positive control):
- - Results with reference substance valid?
yes
- ECx: EC50 at 2.07 mg/L
- Other: 95% confidence limits: 1.22 - 3.66 mg/L - Reported statistics and error estimates:
- No statistical analysis was performed
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the limit concentration of 100 mg/L of the test item, no effects on Daphnia magna were observed.
Based on the nominal concentrations of the test item, the 48 hours-EC10 / 50 / 100 for Daphnia magna was > 100 mg/L. - Executive summary:
In the acute immobilization test with Daphnia magna (STRAUS), the effects of the test item were determined at the test facility according to OECD 202 (2004) from 2018-06-12 to 2018-06-14.
A concentration of 100 mg/L was tested in a limit test under static conditions over a period of 48 hours. The limit concentration of 100 mg test item/L was blue and visually clearly dissolved at the start of the exposure, while the control was colourless and visually clear throughout the exposure. After 24 hours and at the end of the exposure interval a slight sedimentation of the test item was observed.
Twenty daphnids, divided into 4 replicates with 5 daphnids each, were exposed to the limit concentration and the control.
The concentrations of the test item were analytically verified via photometric analysis at a wavelength of 662 nm in the fresh media at the start of the exposure (0 hours) and in the old media at the end of the exposure (48 hours) in the limit concentration and in the control.
The measured concentration of the test item in fresh medium at the start of the exposure (0 hours) was 96% of the nominal value of the limit concentration. At the end of the exposure period (48 hours), the measured test item concentration in the old media was 91% of the nominal value of the limit concentration. Since the measured concentration of the test item did remain stable within ± 20% of the nominal concentration throughout the exposure period, the nominal concentration was used for evaluation.
No effects were found at the limit concentration of 100 mg/L of the test item in Elendt M4 medium.
The validity criteria of the test guideline were fulfilled.
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