Registration Dossier
Registration Dossier
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EC number: 269-847-3 | CAS number: 68345-17-5
Endpoint summary
Administrative data
Description of key information
The test item was found to be not readily biodegradable.
Additional information
- Inoculum blank: containing inoculated medium
- Procedure control: containing inoculated medium and reference item.
- Test item: containing inoculated medium and test item.
- Toxicity control: containing inoculated medium, reference item and test item.
- Abiotic control: containing untreated medium, test item and sterilising agent.
OECD 310
The test item was added to the mineral medium to give a final organic carbon concentration of 20 mg C/L. The organic carbon content (73%) was based on the molecular formula. Since Dimethyl Octenyl Acetate was not sufficiently soluble to allow preparation of a 100-times concentrated stock solution in mineral medium, a known amount of 3.36 μL, corresponding with 2.9 mg Dimethyl Octenyl Acetate was added to the test bottles containing medium with microbial organisms and mineral components (107 mL). The test solutions were continuously shaken during the test, to ensure optimal contact between the test item and the test organisms.
The test was performed in sealed bottles with a headspace of air. The test consisted of five groups:
The CO2 evolution resulting from the aerobic biodegradation of the test item was determined by measuring the inorganic carbon (IC) produced in the test bottles in excess of that produced in blank vessels containing inoculated medium only. Biodegradation was expressed as a percentage of the theoretical maximum IC production, based on the quantity of test item (as organic carbon) initially added.
The relative biodegradation values calculated from the measurements performed revealed 14% biodegradation of Dimethyl Octenyl Acetate (based on IC) at the end of 28-day test period. Thus, the criterion for ready biodegradability (at least 60% biodegradation within a 10-day window) was not met. In the toxicity control less than 25% biodegradation occurred within 14 days (18% at the end of the 28-day test period, based on ThCO2). Therefore, the test item was assumed to have a small inhibiting effect on microbial activity. Since all criteria for acceptability of the test were met, this study was considered to be valid. In conclusion, Dimethyl Octenyl Acetate is designated as not readily biodegradable.
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