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EC number: 429-100-6 | CAS number: 23911-56-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-11-07 to 1997-12-11
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control, 0.01 mg/L, 0.02 mg/L, 0.04 mg/L, 0.08 mg/L, 0.16 mg/L and 0.32 mg/L (Nominal concentrations)
- Sampling method and Sample storage conditions before analysis: not reported - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: preparing a stock solution and then diluted to the desired test concentrations
- Controls: M4-medium - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna STRAUS
- Source: cloned at the federal health office (BGA) in Berlin
- Age at study initiation: 0-24 hours
- Feeding during test :no feeding
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 14.9 °dH
- Test temperature:
- 20.6-20.8 °C
- pH:
- 7.9
- Dissolved oxygen:
- 8.8-9.1
- Salinity:
- n.a.
- Conductivity:
- n.a.
- Nominal and measured concentrations:
- Nominal concentrations: 0 mg/L (control), 0.01 mg/L, 0.02 mg/L, 0.04 mg/L, 0.08 mg/L, 0.16 mg/L and 0.32 mg/L
measured concentrations at 0 h: <0.001 mg/L, 0.01 mg/L, 0.022 mg/L, 0.044 mg/L, 0.090 mg/L, 0.177 mg/L and 0.324 mg/L
measured concentrations at 48 h: <0.001 mg/L, 0.009 mg/L, 0.017 mg/L, 0.043 mg/L, 0.074 mg/L, 0.124 mg/L and 0.269 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: cylindrical test vessels with diameter 4.0 cm, height 6.5 cm
- Type: open
- Volume of solution: 20 mL
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: 0h and 48h
VEHICLE CONTROL PERFORMED: no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.01 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 0.3 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Immobilisation of control:
0%
- Reported statistics and error estimates:
- Probit analysis
- Validity criteria fulfilled:
- yes
- Conclusions:
- The value of 48h-EC50 was determined to be 0.1 mg/L based on measured concentration.
- Executive summary:
There is one study, in which the short-term toxicity of test item to aquatic invertebrates was assessed according to Council Directive 67/548/EEC (1992) and GLP. In a static test, Daphnia magna was exposed to six concentration levels with paralell controls for 48 h. The test media were prepared by diluting a stock solution of 5 mg/L into 0 mg/L (control), 0.01 mg/L, 0.02 mg/L, 0.04 mg/L, 0.08 mg/L, 0.16 mg/L and 0.32 mg/L with a separating factor of 2. Test item concentrations were analytically verified by GC-analysis in fresh media at the start of exposure (0 h) and the end of exposure (48 h) in all concentration levels and the control.
The measured concentrations of the test item in fresh media were <0.001 mg/L (control), 0.01 mg/L, 0.022 mg/L, 0.044 mg/L, 0.090 mg/L, 0.177 mg/L and 0.324 mg/L. The measured concentrations at the end of exposure were <0.001 mg/L (control), 0.009 mg/L, 0.017 mg/L, 0.043 mg/L, 0.074 mg/L, 0.124 mg/L and 0.269 mg/L. After 24 h and 48 h of exposure, immobility was observed in all concentration levels and in the control. The derived EC50 (48 h) was 0.1 mg/L based on the measured concentrations
Reference
Results:
Nominal concentration (mg/L) | Results after 24 hours and 48 hours | ||||||
Immobile daphnids | Oxygen [mg/L] | pH values | Temperature [°C] | ||||
absolute | % | ||||||
24 h | 48h | 24h | 48h | 48h | 48h | 48h | |
control | 0 | 0 | 0 | 0 | 9.1 | 7.9 | 20.7 |
0.01 | 0 | 0 | 0 | 0 | 9 | 7.9 | 20.6 |
0.02 | 0 | 1 | 0 | 5 | 9 | 7.9 | 20.6 |
0.04 | 3 | 5 | 15 | 25 | 8.9 | 7.9 | 20.8 |
0.08 | 2 | 5 | 10 | 25 | 8.8 | 7.9 | 20.7 |
0.16 | 2 | 11 | 10 | 55 | 9 | 7.9 | 20.7 |
0.32 | 0 | 20 | 0 | 100 | 9.8 | 7.9 | 20.6 |
Verification of the test concentration:
Nominal concentration (mg/L) | Measured concentrations | |
0 h | 48h | |
control | <0.001 | <0.001 |
0.01 | 0.01 | 0.009 |
0.02 | 0.022 | 0.017 |
0.04 | 0.044 | 0.043 |
0.08 | 0.09 | 0.074 |
0.16 | 0.177 | 0.124 |
0.32 | 0.324 | 0.269 |
Concentrations were determined via GC-analysis.
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.1 mg/L
Additional information
There is one study, in which the short-term toxicity of test item to aquatic invertebrates was assessed according to Council Directive 67/548/EEC (1992) and GLP.
In a static test, Daphnia magna was exposed to six concentration levels with parallel controls for 48 h. The test media were prepared by diluting a stock solution of 5 mg/L into 0 mg/L (control), 0.01 mg/L, 0.02 mg/L, 0.04 mg/L, 0.08 mg/L, 0.16 mg/L and 0.32 mg/L with a separating factor of 2. Test item concentrations were analytically verified by GC-analysis in fresh media at the start of exposure (0 h) and the end of exposure (48 h) in all concentration levels and the control. The measured concentrations of the test item in fresh media were <0.001 mg/L (control), 0.01 mg/L, 0.022 mg/L, 0.044 mg/L, 0.090 mg/L, 0.177 mg/L and 0.324 mg/L. The measured concentrations at the end of exposure were <0.001 mg/L (control), 0.009 mg/L, 0.017 mg/L, 0.043 mg/L, 0.074 mg/L, 0.124 mg/L and 0.269 mg/L. After 24 h and 48 h of exposure, immobility was observed in all concentration levels and in the control. The derived EC50 (48 h) was 0.1 mg/L based on the measured concentrations.
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