2-tert-butyl-4-methoxyphenol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

Short term toxicity of test chemical were evaluated by observing the effects of chemical on the ability to detach from substrate of Dreissena polymorpha

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

other aquatic mollusc: Dreissena polymorpha

Details on test organisms:

SPECIES (NAME) : Dreissena polymorpha (Zebra Mussel)
Organism Characteristics : 5-8 MM SHELL LENGTH

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

146(136-156) milligrams per liter Calcium ca

Test temperature:

17(16.3-17.4)°Celsius

pH:

8 (7.3-8.7)

Dissolved oxygen:

8.3(6-11.2) milligrams per liter

Nominal and measured concentrations:

2.8-4 mg/L

Details on test conditions:

TEST LOCATION : LAB
Water Conductivity : 260(212-298) micro ohms per centimeter
Water Alkalinity : 109(98-120) milligrams per liter Calcium ca

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.4 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

behaviour

Remarks:

Ability to detach from substrate

Remarks on result:

other: 3.4(2.8-4) mg/L

Validity criteria fulfilled:

no

Conclusions:

The 48 hour EC50 value of test chemical in invertebrate (Dreissena polymorpha) was determine to be 3.4 mg/L on the basis of effect on ability to detach from substrate.

Executive summary:

Short term toxicity of test chemical were evaluated by observing the effects of chemical on the ability to detach from substrate. Test conducted under the static system on 5-8 MM shell length of Dreissena polymorpha. The 48 hour EC50 value of test chemical in invertebrate (Dreissena polymorpha) was determine to be 3.4 mg/L on the basis of effect on ability to detach from substrate. This value indicates that the chemical will exhibit short term toxicity to invertebrates. As per the CLP criteria, this classification amounts to Aquatic Chronic Category 2.

Description of key information

Short term toxicity of test chemical were evaluated by observing the effects of chemical on the ability to detach from substrate. Test conducted under the static system on 5-8 MM shell length of Dreissena polymorpha. The 48 hour EC50 value of test chemical in invertebrate (Dreissena polymorpha) was determine to be 3.4 mg/L on the basis of effect on ability to detach from substrate. This value indicates that the chemical will exhibit short term toxicity to invertebrates. As per the CLP criteria, this classification amounts to Aquatic Chronic Category 2.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

3.4 mg/L

Additional information

Summarized result for the toxicity of test chemical on the mobility of aquatic invertebrates were reviewed and mention as below:

Short term toxicity of test chemical were evaluated by observing the effects of chemical on the ability to detach from substrate. Test conducted under the static system on 5-8 MM shell length of Dreissena polymorpha. The 48 hour EC50 value of test chemical in invertebrate (Dreissena polymorpha) was determine to be 3.4 mg/L on the basis of effect on ability to detach from substrate. This value indicates that the chemical will exhibit short term toxicity to invertebrates. As per the CLP criteria, this classification amounts to Aquatic Chronic Category 2.

 

Above study was supported by the second experimental study from peer reviewed journal. Short term toxicity of test chemical were studied on the mobility of daphnia magna. The 48 hour EC50 value of test chemical in Daphnia magna was determine to be 2.3 mg/L on the basis of mobility. This value indicates that the chemical will exhibit short term toxicity to fish. As per the CLP criteria, this classification amounts to Aquatic Chronic Category 2.

 

Objective of the present study was to evaluate the effect of chemical on aquatic mollusc Dreissena polymorpha. The effective concentration (EC50) value of test chemical in aquatic mollusc [zebra mussels (Dreissena polymorpha)] in a 48 hr study on the basis of effect on attachment was consider to be in the range 19.4 to 29 mg/L. Thus based on the above effects observation, chemical consider to be toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

 

Similar short term toxicity of test chemical were evaluated by observing the effects of chemical on the aquatic mollusc. The lethal concentration (LC50) value of test chemical in Dreissena polymorpha in a 48 hr study on the effect basis of mortality was observed to be 65 mg/L. Thus based on the above effects observation, chemical consider to be toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

 

Based on the above two studies, chemical consider to be toxic and classified as aquatic chronic 2 as per the CLP classification criteria.