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EC number: 236-283-4 | CAS number: 13280-60-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Water solubility
Administrative data
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 - 09 August 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- Version / remarks:
- 1995
- Deviations:
- yes
- Remarks:
- The temperature in the equilibration phase was 19.2–19.8 °C with a mean of 19.5 °C and was thus out of the range of 20 ± 0.5 °C. No impact on the study is expected, as the deviation was minor, and the overall mean was in the range given by the guideline.
- Qualifier:
- according to guideline
- Guideline:
- EU Method A.6 (Water Solubility)
- Version / remarks:
- Regulation (EC) No 440/2008
- Deviations:
- yes
- Remarks:
- The temperature in the equilibration phase was 19.2–19.8 °C with a mean of 19.5 °C and was thus out of the range of 20 ± 0.5 °C. No impact on the study is expected, as the deviation was minor, and the overall mean was in the range given by the guideline.
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- flask method
Test material
- Reference substance name:
- 5-amino-2-nitrobenzoic acid
- EC Number:
- 236-283-4
- EC Name:
- 5-amino-2-nitrobenzoic acid
- Cas Number:
- 13280-60-9
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- 5-amino-2-nitrobenzoic acid
- Reference substance name:
- Water
- EC Number:
- 231-791-2
- EC Name:
- Water
- Cas Number:
- 7732-18-5
- Molecular formula:
- H2O
- IUPAC Name:
- Oxidane
- Reference substance name:
- Unknown impurities.
- Molecular formula:
- Not available as unknown impurities.
- IUPAC Name:
- Unknown impurities.
- Test material form:
- solid
- Details on test material:
- Batch / Lot No. 10044431
Constituent 1
impurity 1
impurity 2
- Specific details on test material used for the study:
- Batch no.: 10044431
Storage: At room temperature, protected from light
Results and discussion
Water solubility
- Key result
- Water solubility:
- >= 1.88 g/L
- Conc. based on:
- test mat. (dissolved fraction)
- Temp.:
- 20 °C
- pH:
- 2.3
- Details on results:
- Preliminary test
This preliminary test indicated a water solubility of 1 to 10 g/l, as about 0.1 g of the test item could be dissolved in 100 ml of water.
Main test
The results are given in the table in the next section. The individual results of the test vessels, which were deemed constant, were averaged and are given in mass per volume of solution. The test is satisfactory if the concentrations of at least the last two vessels do not differ by more than 15%.
Any other information on results incl. tables
HPLC determinations:
Test vessel | Incubation time at 30 °C (d) |
Equilibration time at 20 °C (h) |
pH |
Determined concentration A (g/L) |
Determined concentration B (g/L) |
Determined concentration C (g/L) |
Mean (g/L) |
Considered for calcu- |
No. 1 |
1 |
24 |
2.3 |
1.88 |
1.86 |
1.83 |
1.86 |
yes |
No. 2 |
2 |
24 |
2.3 |
1.94 |
1.92 |
1.92 |
1.93 |
yes |
No. 3 |
3 |
24 |
2.3 |
1.85 |
1.83 |
1.85 |
1.84 |
yes |
|
|
|
|
|
|
Overall Mean (g/L) |
1.88 |
|
|
|
|
|
|
|
Variation (%) |
4.6 |
|
The concentrations of the three vessels differed by 4.6% (validity criterion given by OECD is met).
Applicant's summary and conclusion
- Conclusions:
- The water solubility of 5-Amino-2-Nitrobenzoic Acid (CAS no. 13280-60-9) was determined according to the OECD guideline 105
The water solubility of the test item at 20°C was 1.88 g/L. The pH of the aqueous samples was 2.3. - Executive summary:
The water solubility of 5-Amino-2-Nitrobenzoic Acid (CAS no. 13280-60-9) was determined according to the OECD guideline 105.
Several test vessels containing ultra-pure water mixed with test item in great excess (initial amount of test item about 50 g per liter water) were incubated and stirred for different periods of time at 30 °C (saturation phase), and then transferred to about 20 °C for 24 hours (equilibration phase). Undissolved test item was subsequently separated by centrifugation. The concentration of the test item in the clear aqueous phase was analysed with HPLC.
The determined concentrations were as follows:
1.86 g/l after one day at 30 °C, followed by 24 hours at ca. 20 °C, pH 2.3
1.93 g/l after two days at 30 °C, followed by 24 hours at ca. 20 °C, pH 2.3
1.84 g/l after three days at 30 °C, followed by 24 hours at ca. 20 °C, pH 2.3
No significant variation of the concentrations and pH values was observed in the three respective test vessels after one, two and three days of incubation at 30 °C (followed by 24 hours equilibration at about 20 °C).
The water solubility of 5-Amino-2-Nitrobenzoic Acid (CAS no. 13280-60-9), calculated as the mean of the three test vessels, is 1.88 g/l at a pH of 2.3 and about 20 °C.
The validity criterion was fulfilled.
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