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EC number: 814-072-3 | CAS number: 77614-79-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Mar. 2019 - May 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))
- Version / remarks:
- 2016
- Qualifier:
- according to guideline
- Guideline:
- ISO 11268-2 (Effects of Pollutants on Earthworms. 2. Determination of Effects on Reproduction)
- Version / remarks:
- July 1998
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3,5-bis(difluoromethyl)-1H-pyrazole
- EC Number:
- 814-072-3
- Cas Number:
- 77614-79-0
- Molecular formula:
- C5H4F4N2
- IUPAC Name:
- 3,5-bis(difluoromethyl)-1H-pyrazole
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- - Method of mixing into soil: Preparation of a stock solution of the test item in acetone. Preparation of application solutions depending on the desired concentrations in the final test substrate.
2 mL of the respective application solution were given onto 5 g quartz sand per treatment.
- Controls: Control (quartz sand only) and Solvent control (2 mL actone and quartz sand)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone
- Evaporation of vehicle before use: Yes, evaporation of acetone before mixing the quartz sand into the artificial soil.
Test organisms
- Test organisms (species):
- Eisenia andrei
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Source: Inhouse breeding since several years, Original strain from Forschungsanstalt für Landwirtschaft Braunschweig, Germany
- Age at test initiation (mean and range, SD): 9 - 10 months old.
- Weight at test initiation (mean and range, SD): 340 - 430 mg per worm.
ACCLIMATION
- Acclimation period: 1 day before test start
- Acclimation conditions (same as test or not): same as test
- Health during acclimation (any mortality observed): not reported
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 8 wk
Test conditions
- Test temperature:
- 18.5 - 20.5 °C
- pH:
- 5.54 - 6.25 in all controls and treatments at the start and the end of the study
- Moisture:
- 48.17 - 51.19 in all controls and treatments at the start and the end of the study
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): Non-re-usable plastic boxes (length x width x height ca. 16.5 cm x 12 cm x 6 cm, area approximately 200 cm²
- Amount of soil or substrate: 500 g dry weight artificial soil
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 8
- No. of replicates per vehicle control: 8
PROPERTIES OF SUBSTRATE
- Artificial soil according to Guideline:
Sphagnum peat (shredded): 10 %
Kaolinite clay (content of Kaolinite = 30.2 %): 20 %
Industrial quartz sand (Particle size: 0.20 mm – 0.05 mm = >50 %): 70 %
VEHICLE CONTROL PERFORMED: Yes
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
OTHER TEST CONDITIONS
- Photoperiod: 16 h light and 8 h dark
- Light intensity: 400 – 800 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : number of surviving adult earthworms and weight after further 28 days, number of offspring after 56 days - Nominal and measured concentrations:
- Control, Solvent control, 10, 18 , 32, 56, 100 mg/kg d.w. nominal concentrations
- Reference substance (positive control):
- yes
- Remarks:
- Carbendazim EC 360 G
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- Adult mortality
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- Adult growth
- Duration:
- 56 d
- Dose descriptor:
- EC10
- Effect conc.:
- 50 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 56 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 56 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 56 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- - Mortality at end of exposure period adults, 28 days):
Control: 0 %
Solvent control: 1.3%
10 mg/kg: 2.5 %
18 - 100 mg/kg: 0 %
- Changes in body weight of live adults (% of initial weight) at end of exposure period (28 days):
Control, solvent control, 10, 18, 32, 56, 100 mg/kg: 37.87 %, 33.72 %, 34.69 %, 32.55 %, 31.23 %, 33.72 %, 36.88 %
- No. of offspring produced (mean after 56 days):
Control, solvent control, 10, 18, 32, 56, 100 mg/kg: 297.1, 281.3, 279.0, 264.0, 261.5, 249.8 , 237.3 - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: Significant toxic effects onto reproduction at 2.5 and 5.0 mg reference item / ky dry weight soil
Any other information on results incl. tables
Table 1: Results
Concentration mg /kg soil dw |
Control |
Solvent |
10 |
18 |
32 |
56 |
100 |
Mortality of adult earthworms |
0 |
1.3 |
2.5 |
0 |
0 |
0 |
0 |
Significance (Mortality)* |
-- |
- |
- |
- |
- |
- |
- |
Mean change of body fresh |
37.87 |
33.72 |
34.69 |
32.55 |
31.23 |
33.72 |
36.88 |
Standard Deviation |
6.89 |
4.59 |
7.32 |
12.17 |
16.97 |
16.14 |
12.72 |
Significance |
-- |
- |
- |
- |
- |
- |
- |
Mean number of offspring per |
297.1 |
281.3 |
279 |
264 |
261.5 |
249.8 |
237.3 |
Standard Deviation |
59.8 |
32.4 |
35.5 |
60.6 |
35.5 |
22.6 |
33.1 |
% of Solvent control |
-- |
-- |
99.2 |
93.9 |
93 |
88.8 |
84.4 |
Coefficient of variance (%) |
20.1 |
11.5 |
12.7 |
22.9 |
13.6 |
9.1 |
14 |
Significance (reproduction)*** |
-- |
- |
- |
- |
- |
- |
+ |
* (Multiple Sequentially-rejective Fisher Test After Bonferroni-Holm, one-sided greater, α = 0.05
** (Dunnett`s Multiple t-test, two-sided, α = 0.05), + = significant, - = not significant
*** (William’s t-test, one-sided smaller, α = 0.05), + significant, - not significant
Table 2: Validity criteria for OECD 222.
Criterion from the guideline to be satisfied in the controls |
Outcome |
Validity criterion fulfilled |
Each replicate (containing 10 adults) to have produced ≥ 30 juveniles by the end of the test. |
240 - 406 |
Yes |
The coefficient of variation of reproduction is ≤ 30%. |
20.1 % |
Yes |
Adult mortality over the initial 4 weeks of the test is ≤ 10%. |
0 % |
Yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
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