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EC number: 687-893-6 | CAS number: 1150560-54-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28th August 2018 to 7th September 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: MEP, P.R. China, the Guidelines for the Testing of Chemicals, Effects on Biotic Systems (the Second Edition), No. 202 "Daphnia sp., Acute Immobilisation Test". (2013.8)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- other: EC, DG Health and Consumer Protection: Guidance on analytical quality control & validation procedures for pesticide residue analysis in food & feed SANCO/1257/2013 19/11/13 rev 0
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1-{[2-fluoro-6-(trifluoromethyl)phenyl]methyl}-5-iodo-6-methyl-1,2,3,4-tetrahydropyrimidine-2,4-dione
- EC Number:
- 687-893-6
- Cas Number:
- 1150560-54-5
- Molecular formula:
- C13H9F4IN2O2
- IUPAC Name:
- 1-{[2-fluoro-6-(trifluoromethyl)phenyl]methyl}-5-iodo-6-methyl-1,2,3,4-tetrahydropyrimidine-2,4-dione
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- HPLC - UV
- Details on sampling:
- Analysis frequency: in 0-hour fresh solution and 48-hour old solution
Test solutions
- Details on test solutions:
- Method of preparation of stock solution:
A stock solution of nominal concentration 500 mg/L was prepared with acetonitrile. A weight of 0.05002 g test item was dissolved in acetonitrile to 100 mL
Method of preparation of test solutions:
The stock solution was used to prepare the working standard solutions with acetonitrile of 0.05, 0.1, 0.5, 1.0, 5.0, 10 and 20mg/L
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia of stock culture were originally obtained from the College of Applied Chemistry of China Agricultural University. The test Daphnia were derived from the same healthy pre-cultured population (i.e. showing no signs of stress such as high mortality, presence of males and ephippia, delay in the production of the first brood, discoloured animals, etc.). The pre-cultured animals were maintained in culture conditions (light, temperature, and medium) similar to those to be used in the test. The test animals were less than 24 hours old and not the first brood progeny. The batch used for this definitive test was Daphnia C20180829.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- 24 and 48 hours
Test conditions
- Hardness:
- 140 - 250mg/L as (CaCO3)
- Test temperature:
- 18-22 ± 1 °C
- pH:
- 6.0 - 9.0
- Dissolved oxygen:
- Not less than 3mg/L during test period
- Nominal and measured concentrations:
- 0.1503g of test item was weighed and made up to 1.5L with test water. The solution was stirred for 24 hours at 450rpm and then filtered with a 0.45μm membrane. 150, 203, 274, and 370ml were diluted to 500ml with test water.
The 5 nominal test concentrations used were: 30.0, 40.5, 54.8, 74.0 and 100mg/L.
The measured test concentration in fresh media was 0.349, 0.435, 0.626, 1.018 and 1.231mg/L. The measured test concentration in old media was 0.350, 0.443, 0.613, 1.007 and 1.214mg/L. The overall arithmetic mean determined concentrations was 0.350, 0.439, 0.620, 1.013 and 1.223mg/L. . - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers
- Type: open
- Fill volume: 250ml
- Volume of solution: 100ml
- Aeration: none during the test period
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: water treated with Aquapro Pure Water System
- Total organic carbon: <2mg/L
- Alkalinity: pH 6.0 - 9.0
OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily, 8 hours dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : 24 and 48 hours. Animals not able to swim within 15 seconds after gentle agitation of the test vessel. Any abnormal behaviour or appearance was recorded.
RANGE-FINDING STUDY
- Test concentrations: 3, 10, 30 and 100mg/L
- Results used to determine the conditions for the definitive study: There was no inhibition for 3, 10 and 30mg/L and 100% inhibition for 100mg/L. The results of the preliminary test show that the 48 hour EC50 is within the range 30-100mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.57 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- During the 48-hour exposure period, no immobilisation or visible toxicity signs were observed in the control group and 30.0mg/L group. Immobilisation was observed in groups 40.5, 54.8, 74.0 and 100mg/L.
- Results with reference substance (positive control):
- The 24 hour EC50 was 0.57mg/L with 95% confidence limits 1.06 ~ 1.41 mg/L
- Reported statistics and error estimates:
- Trimmed Spearman-Karber (TSK) method was used to derive the EC50 value and the corresponding 95% confidence limits.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- According to the results and based on the measured concentrations, the 48-hour median affect concentration (EC50) of Methyl Iodouracil to Daphnia magna was 0.57mg/L, with 95% confidence limits of 0.51 ~ 0.64mg/L.
- Executive summary:
An Acute Immobilisation study of Methyl Iodouracil was conducted with Daphnia magna over a 48-hour exposure period.
Based on the results of the preliminary test, the definitive test was carried out at five nominal concentrations of 30.0, 40.5, 54.8, 74.0 and 100mg/L. The solution was filtered using a 0.45μm membrane. A blank control was performed concurrently. 4 replicates were used for the control and test concentrations with 5 daphnids for each replicate. The test was performed with a static water system over 48 hours. Dissolved oxygen and pH were measured at 0 hours and 48 hours during the exposure period in both control and test solutions. The temperature was monitored continuously by automatic temperature monitoring system and recorded.
During the 48-hour exposure period, no immobilisation or visible toxicity signs were observed in the control group and 30.0mg/L group. Immobilisation was observed in groups 40.5, 54.8, 74.0 and 100mg/L.
The concentration of test item was determined in samples of test media by HPLC-UV.
The results showed that for the test solutions of 30.0, 40.5, 54.8, 74.0 and 100mg/L, the measured test concentration in fresh media was 0.349, 0.435, 0.626, 1.018 and 1.231mg/L. The measured test concentration in old media was 0.350, 0.443, 0.613, 1.007 and 1.214mg/L. The overall arithmetic mean determined concentrations was 0.350, 0.439, 0.620, 1.013 and 1.223mg/L.
According to the results and based on the measured concentrations, the 48-hour median affect concentration (EC50) of Methyl Iodouracil to Daphnia magna was 0.57mg/L, with 95% confidence limits of 0.51 ~ 0.64mg/L.
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