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Diss Factsheets
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EC number: 429-630-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16.04.1999 to 23.04.1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- (1984)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- (1992)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- RCC Ltd., Environmental Chemistry & Pharmanalytics Division, CH-4452 Itingen (Switzerland)
Test material
- Details on test material:
- - Name of test material (as cited in study report): Eldew PS-203
- Physical state: pale yellow, oily liquid
- Analytical purity: 100%
- Purity test date: not reported
- Lot/batch No.: 808010
- Expiration date of the lot/batch: 31.03.2000
- Storage condition of test material: test material was stored at room temperature (ca. 20°C), away from direct sunlight
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Ajinomoto Co., Inc., 15-1, Kyobashi 1-chome, Chuo-ku, Tokyo 104-8315 Japan//Batch No.: 808010
- Expiration date of the lot/batch: 31.03.2000
- Purity test date: not reported
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: stored at room temperature at about 20°C, away from direct sunlight
- Stability under test conditions: stability in water: 7 d at room temperature
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Because of the large number of components in the test item, quantification of each individual component could not be performed, but the component of the main component was measured in the single WAF and the control. For the analytical determination duplicate samples from the freshly prepared WAF and the control were taken just before the start of the test. For the determination of the maintenance of the main component under the test conditions, requisite volumes of the freshly prepared WAF and the control were incubated during the test period under the same conditions as in the actual test (but without algae). From these flasks, duplicate samples were taken after the 72 h testing period. All samples were analysed immediately after sampling.
The mean concentrations found in the treatment samples were 0.119 mg/l (sampling day 0) and 0.120 mg/L (sampling day 3). The concentration of the main component was nearly constant during the test period.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A water accommodated fraction (WAF) was prepared (no auxiliary solvent or emulsifier was used) due to the properties of the test item (multi-component test item, containing sparingly soluble components). A WAF with the loading rate of 100 mg/L was used as the only test medium (limit test): a saturated dispersion of the test item was prepared by mixing of the test item into the test water as homogeneously as possible by ultrasonic treatment and by intense stirring at room temperature in the dark for 96 hrs and filtration just before start of the test.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Sammlung von Algenkulturen, Pflanzenphysiologisches Institut der Universität Göttingen (Göttingen, Germany)
- Age of inoculum (at test initiation): 4 d
- Method of cultivation: according to guideline
ACCLIMATION:
- Acclimation period: not necessary
- Culturing media and conditions (same as test or not): same
- Any deformed or abnormal cells observed: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 24 mg CaCO3/L
- Test temperature:
- 22-23°C
- pH:
- at the start: 7.9-8.0
at the end: 8.9-9.0 - Nominal and measured concentrations:
- 100 mg/L nominal
0.119 -0120 mg/L measured - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Type (delete if not applicable): the flasks were covered with glass dishes
- Material, size, headspace, fill volume: glass/50 mL/35 mL/15 mL
- Aeration: not reported
- Type of flow-through (e.g. peristaltic or proportional diluter): test conducted under static conditions
- Initial cells density: 10000 cells/mL
- Control end cells density: 1041600 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic test water according to guideline
- Culture medium different from test medium: no
- Intervals of water quality measurement: The pH values were measured in samples from the single WAF and the control at the start and end of the test. The water temperature was measured at least daily.
OTHER TEST CONDITIONS
- Sterile test conditions: not reported
- Adjustment of pH: no
- Photoperiod: continuous illumination
- Light intensity and quality: 6800-7500 Lux (fluorescent tubes)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : biomass and growth rate
- Determination of cell concentrations: electronic particle counter
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable (limit test)
- Justification for using less concentrations than requested by guideline: limit test
- Range finding study: performed
- Test concentrations: not reported
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Reported statistics and error estimates:
- Student-t-test
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item had no inhibitory effect on the growth rate of Desmodesmus subspicatus during the exposure period of 72 h at a loading rate of 100 mg/L nominal (72 NOEC>=100 mg/L nominal; 72 h EC50>100 mg/L nominal).
- Executive summary:
The toxicity of the test item to the green algae Desmodesmus subspicatus was investigated in a study conducted according to OECD Guideline 201 (Alga, Growth Inhibition Test) and EU Method C.3 (Algal Inhibition test). Due to the properties of the test item (UVCB substance containing sparingly soluble components) a water accommodated fraction (WAF, loading rate: 100 mg/L) was prepared and used as the single exposure concentration in the test. The actual concentration in the test samples was measured by HPLC/UV-Vis. In the freshly prepared WAF the mean concentration was found to be 0.119 mg/L and after 72 h of exposure 0.120 mg/L. No signs of intoxication were determined during the test period. The 72 h NOEC and EC50 based on the growth rate were determined to be >=100 and >100 mg/L nominal, respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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