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EC number: 684-206-1 | CAS number: 258864-54-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-04-02 - 2003-11-12
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- GLP, guideline study; four bacterial strains used instead of five. Triplicate plating used only for vehicle controls
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OTS 798.5265 (The Salmonella typhimurium Bacterial Reverse Mutation Test)
- Deviations:
- not specified
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- trihexyl(tetradecyl)phosphanium chloride
- Cas Number:
- 258864-54-9
- Molecular formula:
- C32H68ClP
- IUPAC Name:
- trihexyl(tetradecyl)phosphanium chloride
- Test material form:
- liquid
- Remarks:
- Homogenous Liquid
Constituent 1
- Specific details on test material used for the study:
- Trade name: CYPHOS IL 101 Phosphonium Salt
Physical state: liquid
Composition of test material, percentage of components: Trihexyl(tetradecyl)phosphonium chloride (258864-54-9), > 95%
Lot/batch No.: WE01-001
Expiration date of the lot/batch: >1 year when stored at room temperature and protected from direct contact with water (hydrophobic)
Method
- Target gene:
- Histidine locus
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix from Aroclor-induced rat liver (from Molecular Toxicology, Inc)
- Test concentrations with justification for top dose:
- 1.00, 3.33, 10.0, 33.3, 100, 333, 1000, 3330, 5000 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: ethanol
- Justification for choice of solvent/vehicle: The test article was observed to form a transparent, colorless, non-viscous solution in ethanol at a concentration of approximately 100 mg per mL.
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- ethanol
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- sodium azide
- benzo(a)pyrene
- other: 2-aminoanthracene, ICR-191
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium; in agar (plate incorporation)
DURATION
- Preincubation period:
- Exposure duration: 72 hours at 37°C
NUMBER OF REPLICATIONS: All doses of test article and positive controls were plated at one plate per dose.Vehicle controls were plated in triplicate.
DETERMINATION OF CYTOTOXICITY
- Method: The condition of the background bacterial lawn is evaluated both macroscopically and microscopically (using a dissecting microscope) for indications of cytotoxicity.
- Evaluation criteria:
- A test material is considered mutagenic if there is a reproducibly increasing dose-response curve of induced revertant colonies for at least 3 test concentrations. The minimal criteria for a positive response are a 2- to 3-fold increase in the number of revertants (at least 15 colonies) over the spontaneous number for the TA1535, TA1537, TA1538 and TA98 strains. In addition, a positive response must not be observed only at concentrations near toxic dose levels.
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA1537, TA1535, TA100, TA98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
Any other information on results incl. tables
Table 1: Mutagenicity assay Results - Individual Plate Counts
Concentration (µg test solution) | TA98 | TA100 | TA1535 | TA1537 | ||||||||||||||||||||
-S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | |||||||||||||||||
0 (vehicle control) | 11 | 11 | 9 | 27 | 26 | 22 | 59 | 66 | 63 | 90 | 93 | 93 | 14 | 7 | 8 | 15 | 14 | 20 | 10 | 7 | 5 | 6 | 5 | 6 |
10±1 | 25±3 | 63±4 | 92±2 | 10±4 | 16±3 | 7±3 | 6±1 | |||||||||||||||||
1.00 | 14 | 20 | 74 | 86 | 6 | 9 | 7 | 7 | ||||||||||||||||
3.33 | 12 | 17 | 74 | 106 | 7 | 10 | 10 | 1 | ||||||||||||||||
10.0 | 4 | 20 | 54 | 79 | 10 | 17 | 9 | 6 | ||||||||||||||||
33.3 | 3 | 20 | 21 | 105 | 3 | 23 | 1 | 5 | ||||||||||||||||
100 | 0 | 14 | 0 | 66 | 0 | 6 | 0 | 4 | ||||||||||||||||
333 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||||||||||||
1000 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||||||||||||
3330 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||||||||||||
5000 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||||||||||||
Positive control | ||||||||||||||||||||||||
2-Nitrofluorene | 243 | - | - | - | - | - | - | - | ||||||||||||||||
benzo[a]pyrene | - | 219 | - | - | - | - | - | - | ||||||||||||||||
Sodium azide | - | - | 1160 | - | 893 | - | - | - | ||||||||||||||||
2-Aminoanthracene | - | - | - | 477 | 58 | - | 80 | |||||||||||||||||
ICR-191 | - | - | - | - | - | - | 942 | - |
Applicant's summary and conclusion
- Conclusions:
- negative with and without metabolic activation
Trihexyltetradecylphosphonium chloride was not mutagenic in a bacterial reverse mutation assay up to cytotoxic concentrations. - Executive summary:
Trihexyltetradecylphosphonium chloride was examined for mutagenicity activity in the Salmonella/Mammalian-Microsome Reverse Mutation Screening Assay (Ames Test). This assay evaluated the test article for its ability to induce reverse mutations at the histidine locus in the genome of specific Salmonella typhimurium tester strains TA-98, TA-100, TA-1535 and TA-1537 both in the presence and absence of an exogenous metabolic activation system.
Positive controls (sodium azide, 2-Nitrofluorene, benzo[a]pyrene, ICR-191 and 2-aminoanthracene) were tested under the same experimental conditions.
No significant increases in revertant colony numbers over control counts were obtained with any of the tester strains following exposure to the test material at levels from 1.00 to 5000 µg per plate, either in the presence or absence of the S-9 mix.
Under the conditions of this study, the test material Trihexyltetradecylphosphonium chloride was devoid of mutagenic activity.
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