Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 812-222-2 | CAS number: 4443-23-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 201-08-14 to 2018-10-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- HPLC-DAD
- Details on sampling:
- - Concentrations: 200 mg/L (nominal) and 0 mg/L
- Sampling method: At the start of the exposure and at renewal (0 and 24 h), samples of the fresh media were taken after preparation of the limit concentration and analyzed. At renewal and at the end of the exposure (24 and 48 hours), samples of the 24-hours old media were taken directly from the test vessels and analyzed.
- Sample storage conditions before analysis: If necessary, all original samples were stored at room temperature before preparation. Prepared samples were stored in an autosampler at room temperature until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A limit concentration of 200 mg/L of the test item was prepared with dilution water prior to the start of the exposure (at day 0) and prior to the renewal of the test solution (at day 1). The test item solution was treated with ultrasound for 5 min at room temperature and stirred thereafter for 1 h with a magnetic stirrer at approximately 1100 rpm at room temperature. As a Tyndall effect was still visible, the stirring period was prolonged to 2 h. Since the Tyndall effect was still present, the solution was treated with ultrasound at room temperature for another 10 min. After that, only a slight Tyndall effect was visible.
- Controls: Dilution water without test item incubated under the same conditions as the test groups. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna STRAUS
- Age of parental stock (mean and range, SD): Less than 24 hours old daphnids from a healthy stock were used for the study. Juvenile daphnids were removed from the culture vessels at the latest 24 hours before the start of the exposure and discarded. The juveniles born within the following period of max. 24 hours preceding the exposure were used for the test. No first brood progeny was used for the test.
ACCLIMATION
- Acclimation period: Acclimatization of the daphnids was not necessary, because the composition of the dilution water was equivalent to the culture medium.
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 259-263 mg CaCO3/L
- Test temperature:
- 20.0 °C ± 1°C
- pH:
- 7.38-7.48
- Dissolved oxygen:
- 7.68-8.33 mg/L
- Nominal and measured concentrations:
- 200 mg/L and 0 mg/L nominal
148 mg/L and < LOQ measured - Details on test conditions:
- TEST SYSTEM
- Test vessel: beakers
- Type (delete if not applicable): loosely covered with glasses
- Material, size, headspace, fill volume: 50 mL capacity, glass, fill volume 20 mL
- Renewal rate of test solution (frequency/flow rate): renewal at 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Culture Medium Elendt M4 according to OECD 202
pH: 8.2 ± 0.8
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 hours light/dark cycle
- Light intensity: Diffuse light, light intensity of max. 1500 lx
VEHICLE CONTROL PERFORMED: no
RANGE-FINDING STUDY
- Test concentrations: 0, 1.0, 10.0, 100 mg/L
- Results used to determine the conditions for the definitive study: No immobilisation was found at the concentrations tested. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 148 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 148 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 148 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 148 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Other biological observations: none
- Mortality of control: none
- Other adverse effects control: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: a slight Tyndal effect was observed at the concentration tested. - Results with reference substance (positive control):
- - Results with reference substance valid?
Yes
- Relevant effect levels:
- ECx: EC50: 2.03 mg/L CI (1.17-3.57 mg/L), validity range: 0.6 - 2.4 mg/L, acc. to AQS P 9/2 (02/2000); clone 5 0.6 - 2.1 mg/L, acc. to OECD 202 (2004); clone A
- Other: - Reported statistics and error estimates:
- The effects of the limit concentration were deduced directly from the observed immobilization rates. The effect levels (EC10 / 50 / 100) are given based on the analytically confirmed nominal concentrations. An EC50-value and confidence intervals were calculated for the reference item by linear dose-response regression with the software GraphPad Prism.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the limit concentration of 148 mg/L (based on geometric mean measured concentrations corresponding to a nominal concentration of 200 mg/L) of the test item 2-Phthalimidoethanesulfonamide (TA-3), no effects on Daphnia magna were observed.
Reference
The validity criteria were fulfilled:
In the control group, no daphnids were immobilized or showed any signs of disease or stress, e.g. discoloration or unusual behavior such as trapping on the surface of the water, during the 48-hour test period (required: not more than 10% of the daphnids immobilized in the control). The dissolved O2concentration in the 24-hours old media was ≥ 7.68 mg/L (required: ≥ 3 mg/L in the 24-hours old media) in the limit concentration and in the control.
Table 1: Measured Concentrations and Percent of Nominal Concentration of the Test Item 2-Phthalimidoethanesulfonamide (TA-3) during the Definitive Test
Sampling date |
0 hours Fresh medium |
24 hours Old medium |
24 hours Fresh medium |
48 hours Old medium |
Geometric mean meas. conc. |
||||
Nominal concentration of the |
2‑Phthalimidoethanesulfonamide(TA-3) |
||||||||
test item [mg/L] |
Meas. conc. [mg/L] |
% |
Meas. conc. [mg/L] |
% |
Meas. conc. [mg/L] |
% |
Meas. conc. [mg/L] |
% |
|
200 |
181 |
91 |
118 |
59 |
181 |
90 |
124 |
62 |
148 |
Control |
< LOQ |
< LOQ |
< LOQ |
< LOQ |
|
Description of key information
In the acute immobilization test with Daphnia magna (STRAUS), the effects of 2-Phthalimidoethanesulfonamide (TA-3) were determined according to OECD 202 (2004). The validity criteria of the test guidelines were fulfilled. In the limit concentration of 148 mg/L (based on geometric mean measured concentrations corresponding to a nominal concentration of 200 mg/L) of the test item 2-Phthalimidoethanesulfonamide (TA-3), no effects on Daphnia magna were observed.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.