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EC number: 271-571-3 | CAS number: 68585-64-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 - 25 May 2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- 2011
- GLP compliance:
- no
- Remarks:
- The study was conducted in full compliance with GLP guidelines with one exception. For more details please refer to field "Any other Information on Material and Methods incl. tables".
Test material
- Reference substance name:
- Dihydrogen bis[P,P-dioctyl diphosphato(2-)-O'',O''''][hydroxyacetato(2-)-O1,O2]titanate(2-), branched and linear
- EC Number:
- 271-571-3
- EC Name:
- Dihydrogen bis[P,P-dioctyl diphosphato(2-)-O'',O''''][hydroxyacetato(2-)-O1,O2]titanate(2-), branched and linear
- Cas Number:
- 68585-64-8
- Molecular formula:
- not applicable (UVCB substance)
- IUPAC Name:
- ({bis[(2-ethylhexyl)oxy]phosphoryl}oxy)[(2-{[({bis[(2-ethylhexyl)oxy]phosphoryl}oxy)(hydroxy)phosphoryl]oxy}-4-oxo-1,3-dioxa-2-titanacyclopentan-2-yl)oxy]phosphinic acid; {[({bis[(2-ethylhexyl)oxy]phosphanyl}oxy)({[({bis[(2-ethylhexyl)oxy]phosphoryl}oxy)(hydroxy)phosphoryl]oxy})[(2-hydroxyacetyl)oxy]titanio]oxy}({bis[(2-ethylhexyl)oxy]phosphoryl}oxy)phosphinic acid
1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 (control), 1.0, 10, and 100 mg/L
- Sampling method: samples were analyzed at 0 h (initiation) and from a composite of spent replicate solutions at 72 h (termination) of the test. At each sampling point, a 5-mL sample was collected from the control and each test substance treatment.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The medium was prepared by the addition of appropriate reagent grade salts to autoclaved reagent water. Reagent water is produced by passing reverse-osmosis water through a series of deionization tanks, a laboratory water purification system consisting of carbon, demineralization, and organic adsorption cartridges, and then through a 0.2-μm filter. A 100 mg/L primary standard test item solution was prepared as water accommodated fraction (WAF) by weighing 0.1001 g of the substance and diluting in a glass bottle with 1.0 L of dilution medium. The solution was covered with black plastic to protect from light and stirred using a magnetic stir bar for approi. 24 h at room temperature, with the stirring adjusted to provide a vortex ≤25% of the solution depth. The bottle opening was covered with Parafilm® sealing film to prevent evaporation and contamination. When stirring was stopped, the solution settled for approx. 1 h prior to dispensing test treatment collections and then approximately 200 mL of the solution was drained and discarded. Appropriate volumes of the primary standard solution were then collected and diluted in 0.25 L of dilution medium to produce the 1.0 and 10 mg/L test solutions. The primary standard solution was used as the 100 mg/L test item solution.
- Controls: The control solution contained dilution medium only.
- Evidence of undissolved material: The control and all test solutions appeared clear and colorless with no visible particulates, surface film, or precipitate throughout the test.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22.8 to 25.3°C
- pH:
- pH at initiation: 6.4 to 7.7
pH at termination: 7.7 to 8.1 - Nominal and measured concentrations:
- Nominal Loading Rates: 0 (control), 1.0, 10, and 100 mg/L
Geometric mean measured concentrations: 0.00337, 0.0116, and 0.0538 mg/L of the diethylhexyl phosphite analytical marker - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass Erlenmeyer flasks
- Type (delete if not applicable): flasks were closed with foam stoppers
- Fill volume: 100 mL
- Initial cells density: 5.0 × 10^3 cells/mL
- Control end cells density: 144 × 10^4 cells/mL
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Freshwater algal medium (FWAM).
OTHER TEST CONDITIONS
- Photoperiod: continuous illumination
- Light intensity and quality: 8,000 ± 800 lux (cool-white fluorescent lighting)
EFFECT PARAMETERS MEASURED.
- Determination of cell concentrations: At 72 h (±1 hour), cell-count density was measured in all replicates of the control and test substance treatments by direct microscopic counting with a hemacytometer.
- Range finding study: yes, non-GLP but no further information given in the report.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The control and all test solutions appeared clear and colorless with no visible particulates, surface film, or precipitate throughout the test.
Any other information on results incl. tables
ANALYTICAL RESULTS
Table 1: Analytical results.
Nominal Loading Rate Concentration [mg/L] |
Measured Concentration as mg/L | ||
0 h | 72 h | Geometric Mean Measured Concentration* |
|
control | <MQL | <MQL | <MQL |
1 | 0.000758 | <MQL | 0.00337 |
10 | 0.00899 | <MQL | 0.0116 |
100 | 0.0957 | 0.0303 | 0.0538 |
* For calculations, ½ MQL (0.000300 mg/L) used where the measured concentration was <MQL.
BIOLOGICAL RESULTS
Table 2: Growth Rate Values from Time Zero during a 72-h Exposure.
Nominal Loading Rate Concentration [mg/L] |
Mean Growth Rate (cells/mL/hour) | % Inhibition |
72 h (%CV) |
72 h | |
control | 0.0784 (CV: 2) |
- |
1 | 0.0781 (CV: 3) |
0 |
10 | 0.0790 (CV: 1) |
-1 |
100 | 0.0767 (CV: 1) |
2 |
No significant reduction in growth rate as compared to the control (Dunnett’s test, p = 0.05).
Further effect results:
- EyC50 > 100 mg/L
- EbC50 > 100 mg/L
- NOEbC = 100 mg/L
- NOEyC = 100 mg/L
VALIDITY CRITERIA
Table 3: Validity criteria for OECD 201.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72-hour test period. |
280 times the initial nominal cell density |
yes |
The mean coefficient of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3, for 72-hour tests) in the control cultures must not exceed 35% |
not specified in report |
|
The coefficient of variation of average specific growth rates during the whole test period in replicate control cultures must not exceed 7% in tests with Pseudokirchneriella subcapitata and Desmodesmus subspicatus. For other less frequently tested species, the value should not exceed 10%. |
2% |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- According to the authors the test was valid. For further details please refer to “Any other information on results incl. tables”.
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