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EC number: 701-266-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02-03-1989 to 28-04-1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Study is complete, was performed under GLP and valid. However no analysis was performed so the actual bio-available fraction is unknown, although the substance is completely soluble at the test concentration. Therefore the use of the data from this study is limited when performing a risk assessment
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Qualifier:
- according to guideline
- Guideline:
- other: S.A.L.M. Kooyman: "Parametric Analysis of mortality rates in bio-assays". Water Research 15(1981)107-119.
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- A stock solution of 25 g/l was prepared by dissolving the test
substance in demineralized water containing concentrated HCl.
The presence of the test substance in the test medium caused a
change of the pH, which was outside the range that can be supported
by the test animal. Therefore, the pH was neutralized
both in the stock solution and in the dilution water to a
value between 7.0 and 7.3. The chosen test concentrations were.
prepared by dilution of the stock solution. - Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- The test organism was Poecilia reticulata (guppy); they were
obtained from the lOB (Instituut Onderzoek Bestrijdingsmiddelen), Wageningen, The Netherlands, and maintained in the
Akzo Research Laboratories Arnhem, dept. CRL according to
Standard Operating Procedures SOP CRL T9 & 42. The size was
approximately 2 cm. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 68.4 mg Ca/l, 35.7 mg Mg/l, 17.7°dH
- Test temperature:
- 21.7-23 C
- pH:
- 7.0-7.7
- Dissolved oxygen:
- 7.1-7.7 mg/L
- Nominal and measured concentrations:
- nominal: 100, 180, 320, 560, 1000 mg/L
- Details on test conditions:
- The temperature was kept between 21.7 and 23.0 C, and the light
regime was 15 hours ambient light per day. The duration of the test was 96 hours.
The test vessels were 2 l glass aquaria, containing 1 l of test medium.
The loading in the test vessels was approximately 0.6 g biomass/l. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.18 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 0.14-0.23 g/L
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.32 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- No mortality occurred at 0.1 g/l, and 100% mortality occurred at 0.32 g/l. The
LC50-96 was 0.18 g/l. The 95% confidence limits are 0.14 and 0.23 g/l.
The quality criteria of this test have been fulfilled: the mortality
in the control was S10%, the oxygen concentration was >60% of
the air-saturation value throughout the test, and the concentration
of PEHA in the test medium remained constant.
The result of the present test are very well in line with earlier
tests. Ten Berge and Blok (1975) found an LC 50 -96 h for guppy of
0.19 g/l with 95% confidence limits of 0.16 and 0.22 g/l. - Reported statistics and error estimates:
- The LC50 was determined with an LC50 program of Griffioen
(R1ZA), based on a model of Kooyman (1981). - Sublethal observations / clinical signs:
Nominal concentration mg/L
Cumulative number of surviving animals
0h
24h
48h
72h
96h
control
30
30
30
30
30
100
10
10
10
10
10
180
10
10
9
9
5
320
10
10
6
2
0
560
10
10
4
1
0
1000
10
10
2
0
0
- Validity criteria fulfilled:
- yes
- Conclusions:
- No significant mortality occurred at 0.1 g/l, and 100% mortality occurred at 0.3
g/l. The LC50 -96 was 0.18 g/l. The 95% confidence limits are 0.14 and 0.23 g/l.
The quality criteria of this test have been fulfilled: the mortality
in the control was <90%, the oxygen concentration was >60% of
the air-saturation value throughout the test, and the concentration
of TEPA in the test medium remained constant. The test is valid. Only nominal concentrations were recorded so this is a limitation when using these values in a riskassessment. - Executive summary:
Pentaethylenehexamine (PEHA) was tested in an acute toxicity test with guppy (Poecilia reticulata). The test was performed according to the EEC method, part C.: Methods for the determination of ecotoxicity, C.1. Acute toxicity to fish.
The test was performed as a semi-static test, in which the solution was renewed after 48 hours. During this time pentaethylenehexamine is not (bio)degradable.
No mortality was observed up to 0.18 g/l. The LC50 was 0.18 g/l, with 95% confidence limits of 0.14 and 0.23 g/l. The quality criteria of this test have been fulfilled: the mortality in the control was S10%, the oxygen concentration was >60% of the air-saturation value throughout the test, and the concentration of PEHA in the test medium remained constant.
Only nominal concentrations were recorded. this limits the usability of the test data for the purpose of riskassessement when looking at the bioavailable fraction .
Reference
Description of key information
LC50(96h):180 mg/L (nominal)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 180 mg/L
Additional information
The acute toxicity of the substance to freshwater fish was tested in a guideline study following EU Method C.1 and S.A.L.M. Kooyman: "Parametric Analysis of mortality rates in bio-assays". Water Research 15(1981)107-119. The semi-static test was conducted with Poecilia reticulata as test organism. The fish were exposed to nominal substance concentrations of 100, 180, 320, 560 and 1000 mg/L for 96 hours. The test solution was renewed after 48 hours. Analytical dose verification was not performed since the substance was expected to be stable under the tested conditions. Mortality was not observed up to a concentration of 180 mg/L. Analytical dose verification was not performed since the substance was expected to be stable under the tested conditions. At test termination a LC50 (96 h) of 180 mg/L (nominal) was determined.
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