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EC number: 220-605-5 | CAS number: 2834-84-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see Remarks
- Remarks:
- This study was performed according with OECD Guideline No. 209 and in accordance with GLP principles. Deficiencies: the pH was not adjusted in the highest test concentration (1000 mg/L). Nonetheless, the test is deemed acceptable as the EC50 exceeded 1000 mg/L.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Guanidinium phosphate (1:1)
- EC Number:
- 226-551-9
- EC Name:
- Guanidinium phosphate (1:1)
- Cas Number:
- 5423-22-3
- Molecular formula:
- CH5N3.H3O4P
- IUPAC Name:
- Guanidium phosphate (1:1)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of 10 g/L was prepared by adding 5.0 g test substance to 500 mL of tap water purified by reverse osmosis. Vigorous mixing (vortex) was used to dissolve the test substance in the test medium. Volumes of the stock solution were then added to the test media to prepare the desired test concentration. Magnetic stirring was applied during the distribution of the stock solution into the test vessels to ensure homogeneity.
- Controls: blank control, nitrification control, positive (reference substance) control and abiotic control
- Evidence of undissolved material (e.g. precipitate, surface film, etc): No
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Method of cultivation: The sludge was coarsely sieved, washed and diluted with ISO-medium. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (3.0 g/L of sludge, as used for the test). The batch of sludge was used one day after collection; therefore 50 mL of synthetic sewage feed was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- Not reported
- Test temperature:
- 19.2 - 20.8 °C
- pH:
- Before addition of sludge: 6.2 - 7.8
End of exposure period: 6.3 - 7.9 - Nominal and measured concentrations:
- - Nominal: blank control, 10, 100 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: All glass, 1 litre test bottles
- Aeration: Clean, oil-free air. The aeration was adjusted in such a way that the dissolved oxygen concentration at the start was above 60-70% saturation (60% of air saturation is > 5 mg/L at 20°C) and to maintain the sludge flocs in suspension
- No. of vessels per concentration (replicates): 3 for the highest test concentration; 1 for the lowest test concentrations
- No. of vessels per control (replicates)
Blank control: 2
Nitrification control: 1
Abiotic control: 1
Positive control (reference substance): 1
- Biomass loading rate (suspended solids): 1.5 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap-water purified by reverse osmosis
- Culture medium different from test medium: Yes
- Intervals of water quality measurement: pH at the begining and the end of the exposure period. Temperature at the end of the exposure period.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Oxygen consumption (respiration rate). After the 3-hour contact time the oxygen consumption was recorded for a period of 15 to 17 minutes. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer. This procedure was repeated for all test/reference substance concentrations and controls. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - The combined limit/range-finding test showed 19%, 22% and an average of 38% inhibition of the respiration rate at 10, 100 and 1000 mg/L, respectively. The EC50 was above the highest concentration rate tested (EC50>1000 mg/L). Since Guanidine phosphate (1:1) decreased the respiration rate of waste water (activated sludge) bacteria at the highest concentration tested, a NOEC could not be determined.
- There was no oxygen uptake from abiotic processes. The results at 1000 mg/L with a nitrification inhibitor showed 39% heterotrophic inhibition of the respiration rate.
- Acceptability of the test:
1. The mean control oxygen uptake rate was 20 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour. The coefficient of variation of oxygen uptake in control replicates did not exceed 30% at the end of the definitive test.
2. The EC50 of 3,5-dichlorophenol was in the accepted range of 2 to 25 mg/L for total respiration (9.4 mg/L) - Results with reference substance (positive control):
- 3,5-dichlorophenol: EC50 was 9.4 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Guanidine phosphate (1:1) significantly inhibited the respiration rate at a concentration of 1000 mg/L. The NOEC could not be determined and the EC50 exceeded 1000 mg/L.
- Executive summary:
In a 3 h activated sludge respiration inhibition study conducted according to OECD guideline 209, microorganisms in activated sludge were exposed to Guanidine phosphate (1:1) at the following nominal concentrations: 0 (blank control), 10, 100 and 1000 mg/L. The test substance significantly inhibited the respiration rate at a concentration of 1000 mg/L. The NOEC could not be determined and the EC50 was >1000 mg/L. The study is considered to be reliable with restrictions since the pH was not adjusted in the highest test concentration (1000 mg/L). Nonetheless, the test is deemed acceptable as the EC50 exceeded 1000 mg/L.
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