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EC number: 271-985-4 | CAS number: 68648-28-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From June, 1988 to July, 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Qualifier:
- according to guideline
- Guideline:
- other: Robra K.H. (1976). Wasser/Abwasser 117:80-86
- Deviations:
- not specified
- Principles of method if other than guideline:
- Bacteriotoxic substances inhibit the bacteria's enzymatic activity, leading to decrease oxygen consumption. The Robra-test measures oxygen consumption of Pseudomonas putida cultures exposed to various concentrations of a test substance and the EC10 or EC50 compared to a negative controlare determined.
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Laurylmyritylalcoholethoxylate 7.5 E.O - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: Pseudomonas DSM putida 50026
- Method of cultivation: in standard nutrient solution
- Preparation of inoculum for exposure: Pre-inoculum cultivated at 1°C for ca. 16 h on a rotary shaker. 10 ml pre-cuture taken and dissolved in 200 ml nutrient solution, shaken at 26°C for ca. 16 h prior to test start. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
- Remarks on exposure duration:
- none
- Nominal and measured concentrations:
- Nominal concentrations: 0, 40,000, 50,000, 60,000, 70,000, 80,000, 90,000 and 100,000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 300 ml Erlenmeyer
- Aeration: with pressurized air - Reference substance (positive control):
- no
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- 67 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Under the study conditions, the EC10 to Pseudomonas putida was estimated to be 67000 mg/L.
- Executive summary:
A study was conducted to evaluate the toxicity of glycerides, C16-18 and C18-unsatd. (alkali-refined linseed oil) to Pseudomonas putida according to the method described in Robra KH (1976), GFW Wasser/Abwasser 117:80 -86. Microorganisms were exposed to test substance concentrations ranging from 40000 to 100000 mg/L and oxygen consumption was measured. Under the study conditions, the EC10 to Pseudomonas putida was estimated to be 67000 mg/L (Kolvenbach, 1988).
- Endpoint:
- activated sludge respiration inhibition testing
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- the study does not need to be conducted because the substance is found to be readily biodegradable and the applied test concentrations are in the range of concentrations that can be expected in the influent of a sewage treatment plant
- Reason / purpose for cross-reference:
- data waiving: supporting information
Referenceopen allclose all
Description of key information
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 22.8 mg/L
Additional information
Toxicity to aquatic microorganisms was assessed based on studies for substances representative of the main constituents, which can be categorised as pentaerythritol esters (PE) and glycerol esters (GE). The results are presented below:
Pentaerythritol esters (PE)
The study does not need to be conducted because the substance, Fatty acids, C14 -18 and C18 -unsatd., branched and linear, esters with pentaerythritol were readily biodegradable in an OECD Guideline 301B study (76.5% degradation after 28 d). The applied test concentration in this study were in the range of those that can expected for the test substance in the influent of a sewage treatment plant. Therefore, the NOEC for toxicity to microorganisms can be considered to be at 22.8 mg/L
Glycerol esters (GE)
A study was conducted to evaluate the toxicity of glycerides, C16-18 and C18-unsatd. (alkali-refined linseed oil) to Pseudomonas putida according to the method described in Robra KH (1976), GFW Wasser/Abwasser 117:80 -86. Microorganisms were exposed to test substance concentrations ranging from 40000 to 100000 mg/L and oxygen consumption was measured. Under the study conditions, the EC10 to Pseudomonas putida was estimated to be 67000 mg/L (Kolvenbach, 1988).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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