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EC number: 294-955-2 | CAS number: 91771-62-9 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Cyperus scariosus, Cyperaceae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Vapour pressure
Administrative data
Link to relevant study record(s)
- Endpoint:
- vapour pressure
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 104 (Vapour Pressure Curve)
- Version / remarks:
- EEC A.4
OECD 104 - GLP compliance:
- no
- Type of method:
- gas saturation method
- Specific details on test material used for the study:
- Name: Cypriol (Cyperus Scariosus ext. oil)
Bath No. 20016011417
Purity 100 % Natural
Manufactured date November 2016
Retest Date October 2018 - Key result
- Temp.:
- ca. 20 °C
- Vapour pressure:
- ca. 1.727 other: mPa
- Conclusions:
- The vapour pressure of test substance was observed 1.7266 mPa at 20°C
- Executive summary:
The vapour pressure of test substance was observed 1.7266 mPa at 20°C
Reference
Method Validation
The analytical method for the determination of test item concentration was validated by establishing linearity, range, accuracy, precision, specificity, Limit of Detection (LOD) and Limit of Quantitation (LOQ).
The results of methodvalidationare
presented in Table 1 and
Table 2.
The chromatograms of the detector linearity check are presented in Figure 1 and Figure 2anda graph of detector linearity check is presented as Figure 3.
The chromatograms of method accuracy of method validation test are presented as Figure 4 and Figure 5. The chromatogram of specificity of methodvalidationtest is presented as Figure 6.
Detector linearity
A series of working standard solutions of concentration 5.247 to 53.000 µg/mL were analyzed in triplicate by HPLC. The detector response found linear in this concentration range with a correlation coefficient (r) of 0.99976 (Table 1).
Limit of Detection (LOD) for the Method
The minimum quantity of the standardof 0.318µg/mL, was detected by the HPLC with signal to noise ratio of 4.55, was considered as Limit of Detection (LOD) of equipment.
From the above equipment LOD, which was in µg/mL of solution, the % method LOD, i.e. µg of analyte in 100 µg of sorbent was calculated and was found to be 0.28%
Limit of Quantification (LOQ) of the Method
The lowest concentration of the reference standard of1.040µg/mLanalysed using the method being validated.Further, at this concentration the percent relative standard deviation (% RSD) of the peak area of three measurements was1.39 % which was less than the value of 10.72 % obtained using extended Horwitz equation. The signal to noise ratio of the analyte peak at this concentration was >10.
Accuracy, Precision and Specificity
Accuracy as mean per cent recovery of test item found to be94.71±0.27and91.27±0.57for low and high dose, respectively (Table 2). This considered acceptable as the obtained mean percent recovery within 80 to 120.
The precision of the method at low and high dose of test item found to be0.29and0.62respectively (Table 2). This considered acceptable as the obtained % rsd less than 5.60 and 3.96 for low dose and high dose, respectivelyobtainedasper % rsd < 2(1 – 0.5 log C)× 0.67.
Specificity of the method considered acceptable since, the control sample (unfortified) did not show any interference at the retention time of the analyte.
System Suitability Test
System precision checked by calculating relative standard deviation (% RSD) from the peak area values and retention time of all replicate injections of a working standard solution and less than 2.0% in each case. Acetonitrile, injected at the end of system suitability test, did not show injection carryover
Vapour Pressure
The measured quantities of active ingredient content in samples are presented in Table 3.
The amount of active ingredient of the test item transported by the inert gas adsorbed in the first and second layer in all the tests. Thesecond layer of 120°C top and third layers of all the test temperatures did not contain any active ingredient. This showed that the adsorbent efficient in adsorbing the entire transported test item. The vapour pressure at different temperatures calculated. The results on correction of flow rate for pressure and temperature difference and volume of saturated gas and vapour pressure are presented in the Table 4, Table 5, Table 6 and Table 7.
Vapour Pressure at 120°C
The vapour pressure of the test item determined at 120.2°C for three different gas flow rates (5, 10, and 15 mL/min). The vapour pressure of test item 6.4172 × 10-1, 5.5815 × 10-1and 6.1466 × 10-1Pa, at 5, 10 and 15 mL/min flow rates, respectively with a mean value of 6.0484 × 10-1Pa (Table 8). The chromatogram of test sample of 120º C is presented as Figure 10 and Figure 18.
Vapour Pressure at 130°C
The vapour pressure of the test item determined at 130.1°C for three different gas flow rates (5, 10 and 15 mL/min). The vapour pressure of test item 9.4251 × 10-1, 8.4889 × 10-1and 9.0201× 10-1Pa, at 5, 10 and 15 mL/min flow rates, respectively with a mean value of 8.9780 × 10-1Pa (Table 8). The chromatogram of test sample of 130º C is presented as Figure 19 and Figure 27.
Vapour pressure at 140°C
The vapour pressure of the test item determined at 140.1°C for three different gas flow rates (5, 10 and 10 mL/min). The vapour pressure of test item 1.42174, 1.30661and 1.33634 Pa, at 5, 10 and 15 mL/min flow rates, respectively with a mean value of 1.35490 Pa (Table 8). The chromatogram of test sample of 140º C is presented as 28 and Figure 36.
Vapour Pressure curve
The vapour pressure curve plotted using inverse value of
experimental temperature in K (1/T) on X- axis and log p (where
p = vapour pressure (Pa) determined at different temperatures) on
Y-axis Figure37).
Vapour Pressure at 20 and 25°C
The vapour pressure of the test item at 20 and 25°C extrapolated from the vapour pressure curve (Figure 37) using slope (--2919.1667) and intercept (7.1951). The calculated vapour pressure of test item at 20 and 25°C 1.7266 mPa and 2.5363 mPa, respectively (Table 8).
Description of key information
The vapour pressure of test substance was observed 1.7266 mPa at 20°C
Key value for chemical safety assessment
Additional information
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