Reaction mass of 2-O-α-L-Rhamnopyranosyl-α-L-rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoic acid and α-L-Rhamnopyranosyl-β-hydroxydecanoyl-β-hydroxydecanoic acid

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

yes

Remarks:

see below 'Principles of method if other than guideline'

Principles of method if other than guideline:

The typical methods proposed by the OECD are:
a) Column elution method
b) Flask method.
After pre-tests both, method a) and method b), could not be directly applied on the specific requirements of this compound/mixture. So an acceptable modification of this proposed methods was necessary. Based on this the laboratory established an appropriate method for the determination of water solubility.

GLP compliance:

no

Other quality assurance:

ISO/IEC 17025 (General requirements for the competence of testing and calibration laboratories)

Type of method:

other: visual method

Key result

Water solubility:

680 g/L

Temp.:

25 °C

Remarks on result:

other: pH unknown

Details on results:

- solvent: 5 mL water
- dissolved compound 3400 mg within four days
- higher amounts result a „suspension“

Conclusions:

680 g/L at 25°C

Executive summary:

The column elution and the shake flask method according to OECD 105 proved to be not applicable for the substance 'Decanoic acid, 3-[[6-deoxy-2-O-(6-deoxy-α-L-mannopyranosyl)-α-L-mannopyranosyl]oxy]-, 1-(carboxymethyl)octyl ester, mixt. with 1-(carboxymethyl)octyl 3-[(6-deoxy-α-L-mannopyranosyl)oxy]decanoate'. Therefore, the water solubility was estimated by the following procedure: The dissolution of the test compound was realised in the first steps with the application of each 200 mg of powdered substance into the solvent/solution to induce primary a dissolution in an ultrasonic bath. After a few steps the viscosity increased, so that the treatment with ultrasonic waves showed no effect. The only possible treatment was a carefully shaking process over a long time period (about 4h each). After this time a dissolution could be detected as a missing of solid particles in the high viscous clear solution. The end point was achieved if no more solid compound could be dissolved.

The water solubility of the test material was determined to be 680 g/L at 25°C.

Description of key information

water solubility of the test material: 680 g/L at 25°C

Key value for chemical safety assessment

Water solubility:

680 g/L

at the temperature of:

25 °C