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Diss Factsheets
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EC number: 913-660-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01/2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of glycerol and N-[3-(dimethylamino)propyl]stearamide and N-[3-(dimethylamino)propyl]docosanamide
- Molecular formula:
- C21 H50 N O C23 H54 N O C25 H58 N O C27 H62 N O
- IUPAC Name:
- Reaction mass of glycerol and N-[3-(dimethylamino)propyl]stearamide and N-[3-(dimethylamino)propyl]docosanamide
- Test material form:
- solid: flakes
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- Anabaena sp.
Study design
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Details on test conditions:
- pH range : 8.2 ± 1.5
Incubation Temperature : 21–24°C controlled at 2 °C
Initial Biomass concentration weight : 104 cells per ml or 0.5 mg/l
Illumination : 2960- 4440 lux
Growth medium : BG-11 Medium
Apparatus to determine algal biomass : UV-Vis Spectrophotometer
No. of replicates : Five in control and three in test groups - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- >= 100 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- >= 100 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 100 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- Since no growth inhibitions were found in the range finding study, thus the limit test
was conducted at the maximum allowable concentration of 100 mg/l of ‘Brassicamidopropyl
Dimethylamine’ in BG-11 medium. The pH measurement in control and ‘Brassicamidopropyl
Dimethylamine’ concentrations are presented in Table 2. Initial pH of BG-11 medium were
in the range of 8.22 – 8.32 and the final pH were recorded in the range of 6.75-6.78 at the
test termination. The test conditions (Temperature and Light intensity of the experimental
room) during the test period are presented in Table 3. During the test, the temperature was
maintained at the range of 23.6-24.1°C and the light intensity was maintained within the
range of 4252 to 4265 lux.
The algae absorbances (optical density) in control and test item concentrations of
‘Brassicamidopropyl Dimethylamine’ are given in Table 4.
Percent growth inhibition of yield and specific growth rate based on control at 0-72
hours of ‘Anabaena sp.’ exposed to various concentrations of ‘Brassicamidopropyl
Dimethylamine’ are given in Table 5.
The maximum concentration of the test item ‘Brassicamidopropyl Dimethylamine’
did not elicit any inhibitory effects on the yield and specific growth rate of algal strain
‘Anabaena sp.’. Therefore, the effective concentrations (ECs) and no observed effect
concentration (NOEC) came out as:
EC10 100 mg/ l
EC20 100 mg/ l
EC50 100 mg/ l
NOEC 100 mg/ l
The percentage inhibition and specific growth rate are calculated by comparing with
the solvent control. The percent coefficient of variation (% CV) at different time interval in
the replicate control flasks was calculated as 18.87% which is less than 35%. Hence, the
result of study complies with the requirements of the OECD Guideline.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the condition of this test, it was concluded that maximum concentration of the test
item ‘Brassicamidopropyl Dimethylamine’ i.e. 100 mg/l elicited no inhibitory effects on the
yield and specific growth rate of algal strain ‘Anabaena sp.’. Therefore the effective
concentrations (ECs) and no observed effect concentration (NOEC) came out as:
EC10 >= 100 mg/ l
EC20 >= 100 mg/ l
EC50 >= 100 mg/ l
NOEC >= 100 mg/ l
The study was conducted as per the procedure described in the OECD Guideline for the
testing of Chemicals, Freshwater Algae and Cyanobacteria, Growth Inhibition Test Guideline
No. 201 Adopted on 28th July 2011. - Executive summary:
The study was conducted to determine the growth inhibition effect of ‘Brassicamidopropyl
Dimethylamine’ on algal strain ‘Anabaena sp.’.
A range finding study was conducted at four concentrations 1 mg/l, 10 mg/l, 50 mg/l & 100
mg/l of test item. No significant growth inhibition was found at any concentration.
Therefore, on the basis of range finding study, a limit test was conducted using
concentration of 100 mg/l in BG-11 medium.
The temperature was maintained within the range of 23.6-24.1°C and light intensity within
the range of 4252-4265 Lux throughout the experimentation.
Under the condition of this test, it was concluded that ‘Brassicamidopropyl Dimethylamine’
elicited no inhibition effects on the yield and specific growth rate of algal strain ‘Anabaena
sp’. Therefore, the Effective concentrations (ECs) and No observed effect concentration
(NOEC) came out as:
EC10 >= 100 mg/ l
EC20 >= 100 mg/ l
EC50 >= 100 mg/ l
NOEC >= 100 mg/ l
This study was hence, performed to assess the ‘Freshwater Algae and Cyanobacteria,
Growth Inhibition Test with “Brassicamidopropyl Dimethylamine”
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