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EC number: 201-782-8 | CAS number: 87-90-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- A 48 h acute toxicity study was performed with cyanuric acid. Procedures were based on “Methods for acute toxicity tests with fish, macroinvertebrates and amphibians” (US EPA 1975).
- GLP compliance:
- no
- Analytical monitoring:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: Cultured at E G and G Bionomics
- Age at study initiation: ≤ 24 hours old
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 60 mg/L CaCO3
- Test temperature:
- 22 ± 1 ºC
- pH:
- 7.0– 7.3
- Dissolved oxygen:
- 6.8 mg/L (77 % saturation) to 8.3 mg/L (94 % saturation)
- Nominal and measured concentrations:
- 1,000, 600, 360, 220, 130 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type open
- Material, size, headspace, fill volume: 250 ml beakers containing 150 ml of test solution
- No. of organisms per vessel: 5 animals / vessel
- No. of vessels per concentration (replicates): 3 vessels / concentration
- No. of vessels per control (replicates): 3 vessels / concentration
- Biomass loading rate: 30 mL / animal
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionized, reconstituted well water
OTHER TEST CONDITIONS
- Adjustment of pH: The appropriate amount of the test compound was dissolved in water and neutralized to pH 7 with 1N NaOH
EFFECT PARAMETERS MEASURED : Mortality was determined at 24 and 48 hours
- Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Behavioural abnormalities: None
- Mortality of control: No mortalities - Validity criteria fulfilled:
- no
- Remarks:
- Solution concentrations were not measured
- Conclusions:
- Cyanuric acid is not harmful to Daphnia magna
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- Method: other: U.S. Environmental Protection Agency "Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms" EPA/600/4-90/027
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Solutions prepared by addition of cyanuric acid to test concentrations containing 1200 ml of synthetic saltwater. The pH of the 8000 mg/l high concentration was raised to 7.0 by addition of 1.0 N NaOH, then stirred for 1/2 hour on a magnetic stir plate. The solution was then placed in a sonic water bath until the cyanuric acid had dissolved into the synthetic seawater. - Test organisms (species):
- Americamysis bahia (previous name: Mysidopsis bahia)
- Details on test organisms:
- TEST ORGANISM
- Common name: Mysid shrimp
- Source: Obtained from a commercial supplier
- Age at study initiation: 3 days
ACCLIMATION
- Acclimation period: Held and acclimated for 1 day in the test dilution water
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: Artemia
- Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 522 - 1560 as CaCO3 mg / L
- Test temperature:
- 24.4 - 25.3 (mean: 24.8)
- pH:
- 6.86 – 8.09
- Dissolved oxygen:
- 7.6 - 8.3 mg/l
- Nominal and measured concentrations:
- 0, 500, 1000, 2000, 4000 and 8000 mg/l (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: During test the vessels were kept covered except when experimental observations were made
- Material, size, headspace, fill volume: 250 ml glass beakers filled with 200 ml of dilution water
- Aeration: Aerated and aged for more than 24 h prior to using in the test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic sewater prepared by addition of reagent grade marine salt to Millipore water.
- Intervals of water quality measurement: Day 0 -temperature, specific conductivity/salinity, total hardness and alkalinity, pH and dissolved oxygen, daily - temperature, dissolved oxygen, conductivity/salinity, and pH.
OTHER TEST CONDITIONS
- Adjustment of pH: Test solution adjusted toa pH of 7.00, placed on a stir plate, then placed in a sonic waterbath
- Photoperiod: 16 h light, 8 h dark, maintained using cool–white fluorescent lamps.
EFFECT PARAMETERS MEASURED : Daily - test organisms and abnormal behaviour and signs of stress, and dead test organisms
- Reference substance (positive control):
- yes
- Remarks:
- potassium chloride
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4 438 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL (3828-5145 mg/L)
- Results with reference substance (positive control):
- LC50: from 0.62 to 1.41 g/L over 8 tests
- Reported statistics and error estimates:
- The LC50 was calculated via the Spearman-Karber method; LOEC and NOEC were analyzed using Fisher’s exact test.
- Conclusions:
- Cyanuric acid is not harmful to mysid shrimp
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- Procedure based on Standard Methods for the Examination of Water and Wastewater. American Public Health Association, 1975.
- GLP compliance:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: Cultured at the ABC facilities
- Age at study initiation: First instar was less than 18 h old
- Feeding during test: No - the adult daphnia were fed a suspension of trout chow and alfalfa (PR-11) daily until 24 hours prior to testing
- Test type:
- static
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 220 mg/l
- Test temperature:
- 19°C
- pH:
- 7.7
- Dissolved oxygen:
- 8.6 mg/l
- Nominal and measured concentrations:
- 0, 560, 1000, 1800, 3200 and 5600 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: 500 ml glass beakers containing 250 ml of ABC well water
- No. of organisms per vessel: 10 Daphnia/vessel
- No. of vessels per concentration (replicates): 2 vessels/concentration
- No. of vessels per control (replicates): 2 vessels
- Biomass loading rate: 25 ml/Daphnia
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Well water from Analytical Biochemistry Laboratories
- Alkalinity: 210 mg/l
- Intervals of water quality measurement: Measured at the termination of the test
OTHER TEST CONDITIONS
- Photoperiod: 16 h daylight, 8 h darkness
EFFECT PARAMETERS MEASURED : Mortality measured at 24 and 48 h
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: A preliminary test using a duplicate of 1500 mg/l concentration exhibited no toxicity - Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- - Behavioural abnormalities: None
- Mortality of control: No mortalities
A white precipitation was observed in all concentrations greater than 1000 mg/L. The 48 h LC50 value quoted in the report was 6000 mg/L. However this value is considered above the reported solubility of cyanuric acid. A precise LC50 value can therefore not be given. The 48 h LC50 is therefore considered to be >1000 mg/L. No mortalities were observed at this concentration. - Reported statistics and error estimates:
- LC50 values were calculated using the technique of Litchfield J.T. and Wilcoxon F (1949)
- Validity criteria fulfilled:
- no
- Remarks:
- Test concentrations not analyzed
- Conclusions:
- Cyanuric acid is not harmful to Daphnia magna
Referenceopen allclose all
Description of key information
Two acute toxicity studies are available for Daphnia magna. The 48 -h EC50 was > 1000 mg/L.
One study is availble for the marine invertebrate mysid shrimp. The 96 -h LC50 was 4438 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1 000 mg/L
Marine water invertebrates
Marine water invertebrates
- Effect concentration:
- 4 438 mg/L
Additional information
In a 48 h static acute toxicity test (LeBlanc 1978) Daphnia magna were exposed to nominal concentrations of 1,000, 600, 360, 220, 130 and 100 mg/L cyanuric acid. No mortalities occurred at the highest concentration test (48 h LC50 = > 1000 mg/L). In another 48 h static acute toxicity study (McAllister 1978) Daphnia magna were exposed to nominal test concentrations of 0, 560, 1000, 1800, 3200 and 5600 mg/L. The 48 h LC50 was determined to be 6000 mg/L. A white precipitation was observed in all concentrations greater than 1000 mg/L. The 48 h LC50 value quoted in the report was 6000 mg/L. However this value is considered above the reported solubility of cyanuric acid. The 48 h LC50 is therefore considered to be >1000 mg/L. No mortalities were observed at this concentration.
In an acute toxicity study with marine aquatic invertebrates (Anderson 2002) mysid shrimp were exposed to nominal concentrations of 0, 500, 1000, 2000, 4000 and 8000 mg/L cyanuric acid for 96 h. The 96 h LC50 was calculated to be 4438 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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