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EC number: 426-540-0 | CAS number: 2973-59-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1998-02-11 to 1998-04-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Swiss Federal Department of the Interior
Test material
- Reference substance name:
- -
- EC Number:
- 426-540-0
- EC Name:
- -
- Cas Number:
- 2973-59-3
- Molecular formula:
- C8 H7 Br O3
- IUPAC Name:
- 2-bromo-5-hydroxy-4-methoxybenzaldehyde
- Details on test material:
- - Name of test material (as cited in study report): T002019
- Molecular formula (if other than submission substance): not applicable
- Molecular weight (if other than submission substance): not applicable
- Smiles notation (if other than submission substance): not applicable
- InChl (if other than submission substance): not applicable
- Structural formula attached as image file (if other than submission substance): not applicable
- Substance type: no data
- Physical state: solid
- Analytical purity: 91.3% (HPLC purity)
- Impurities: no data
- Composition of test material, percentage of components: no data
- Isomers composition: no data
- Purity test date: no data
- Lot/batch No.: 00265786
- Expiration date of the lot/batch: 1998-07-01
- Stability under test conditions: stable
- Storage condition of test material: at room temperature (approx. 20 deg C) away from direct sunlight
- Other: Solubility in water = 70 mg/L; pH of 7.8 at 70 mg/L
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 00265786
- Expiration date of the lot/batch: 1998/07/01 (retest date)
- Purity : 91.3% (HPLC)
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature at about 20°C, in the dark
- Solubility and stability of the test substance in the solvent/vehicle: solubility in water: 70 mg/L
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Duplicate samples at the start of test; duplicate samples from the 10 and 22 mg/L at 72 and 48 h, respectively, because of 100% mortality, and duplicate samples from the 1.0, 2.2, and 4.6 mg/L at 96 h.
- Sample storage conditions before analysis: Deep frozen at -20°C until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 40 mg/L nominal was prepared by dissolving 200 mg test substance in 4.95 L deionized water with treatment in an ultrasonic bath for 2x15 minutes, adding the appropriate analytical grade salts for reconstituted water and intensely stirred for 10 min. Dilutions were made for appropriate test concentrations.
- Eluate: not applicable
- Differential loading: not applicable
- Controls: dilution water only
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc): The test media of the 22, 10 and 4.6 mg/L test concentrations remained colored solutions throughout the entire test duration or the exposure period, and the test media of the 2.2 and 1.0 mg/L test concentrations remained colorless solutions throughout the entire test duration.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Strain: not applicable
- Source: Zoohaus Schaub, CH-4410, Leistal, Switzerland
- Age at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): mean of 10 fish: 2.8 ± 0.2 cm
- Weight at study initiation (mean and range, SD): mean of 10 fish: 0.20 ± 0.07 g
- Method of breeding: obtained from commercial breeder
- Feeding during test: not fed during test
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): yes
- Type and amount of food: Tetra min Hauptfutter, TETRA-Werke,D-49304 Melle, Germany) commercial fish diet, amount unspecified
- Feeding frequency: daily, until one day prior to test
- Health during acclimation (any mortality observed): no mortality
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- Initial: 22 deg C
Final: 21 deg C - pH:
- Initial: 8.1-8.5
Final: 8.0-8.1 - Dissolved oxygen:
- Initial:8.7-8.9 mg/L
Final: 8.7-8.9 mg/L - Salinity:
- not applicable
- Nominal and measured concentrations:
- Nominal: Control, 1.0, 2.2, 4.6, 10, and 22 mg/L
Mean (geometric) measured: Control, not determined, 0.6, 2.0, 6.2, and 19.8 mg/L.
The analytical mean concentrations in fresh solutions were 90-101% of the nominal concentrations. After 96 h, a concentration-dependent decrease was noted to 80, 38, 19, and 8% for the 22, 10, 4.6, and 2.2 mg/L test concentrations, respectively. Therefore, all reported results are based on the mean (geometric) measured concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass aquaria, 4L test solution
- Aeration: yes, slight
- Type of flow-through (e.g. peristaltic or proportional diluter): not applicable
- Renewal rate of test solution (frequency/flow rate): not applicable
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): not applicable
- Biomass loading rate: no data
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water made from analytical grade salts added to deionized water and aerated to saturation.
- Total organic carbon: no data
- Total organic carbon: no data
- Particulate matter: no data
- Metals: no data
- Pesticides: no data
- Chlorine: no data
- Alkalinity: 0.8 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: no data
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
- Light intensity: no data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- signs of intoxication and mortality measured at 2.5, 24, 48, 72, and 96 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study
- Test concentrations: not specified
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 6.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 6.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: The 95% CL could not be calculated.
- Details on results:
- - Behavioural abnormalities: fish mainly at the water surface, apathy, tail-dominant swimming, fish at the bottom of the aquarium, tumbling during swimming
- Observations on body length and weight: not applicable
- Other biological observations: none
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: none - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- The NOEC, LOEC, LC0, and LC100 were determined directly from the raw data and expressed as nominal and mean geometric measured concentrations. The LC50 was calculated after Finney.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Not applicable
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of T002019 to zebra fish was determined in a 96-hour static test according OECD guideline 203. Under the conditions of the present study, the 96-h LC50 of the test substance was 3.4 mg T002019/L (mean measured concentration). The 96-h NOEC was 2.0 mg T002019/L (mean measured concentration). The results of the test can be considered reliable without restriction.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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