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EC number: 200-824-2 | CAS number: 74-95-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- exposure-related information
Reference
- Endpoint:
- in vivo insect germ cell study: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Reason / purpose for cross-reference:
- exposure-related information
- Reason / purpose for cross-reference:
- exposure-related information
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Flies were kept in flasks containing approximately 8 ml of standard Drosophila melanogaster medium into which the desired amount of test material (non-specified purity, DBM) was injected through a rubber septum. The SLRL tests were carried out with treated adult male Drosophila melanogaster flies of a wild-type strain (Berlin K) that were 1-3 days old at the beginning of treatment. The treated males were mated with 3 virgin females of the Base strain and subsequently re-mated every 2 or 3 days with 3 fresh virgins up to a maximum of 5 such mating periods (broods). After short-term exposure, this mating procedure allows the separate sampling of germ-cell populations treated at different stages of their development.
- GLP compliance:
- no
- Type of assay:
- Drosophila SLRL assay
- Species:
- Drosophila melanogaster
- Sex:
- male/female
- Vehicle:
- standard Drosophila medium
- Details on exposure:
- Groups of 20-25 flies were kept in 1160 ml flasks containing approximately 8 ml of standard Drosophila medium into which the desired amount of test material (non-specified purity, DBM) was injected through a rubber septum. When treatment times exceeded 48 hours, the vessels were flushed every 2 or 3 days (fresh medium was given in the case of adult treatment) and a new dose of test material was introduced. The SLRL tests were carried out with treated adult male Drosophila melanogaster flies of a wild-type strain (Berlin K) that were 1-3 days old at the beginning of treatment. The treated males were mated with 3 virgin females of the Base strain and subsequently re-mated every 2 or 3 days with 3 fresh virgins up to a maximum of 5 such mating periods (broods). After short-term exposure, this mating procedure allows the separate sampling of germ-cell populations treated at different stages of their development.
- Tissues and cell types examined:
- For the detection of SLRL mutations, the standard scheme was performed.
- Sex:
- male/female
- Genotoxicity:
- negative
- Conclusions:
- Interpretation of results: negative. DBM is not mutagenic when evaluated in the Drosophila melanogaster sex-linked recessive lethal test.
- Executive summary:
In order to detect the occurrence of mutations, both point mutations and small deletions, in the germ line of an insect, the sex-linked recessive lethal (SLRL) test using Drosophila melanogaster was conducted.The inhalation route was selected by analogy to the human exposure situation.
Groups of 20-25 flies were kept in 1160 ml flasks containing approximately 8 ml of standard Drosophila medium into which the desired amount of test material (non-specified purity, DBM) was injected through a rubber septum. When treatment times exceeded 48 hours, the vessels were flushed every 2 or 3 days (fresh medium was given in the case of adult treatment) and a new dose of test material was introduced. The SLRL tests were carried out with treated adult male Drosophila melanogaster flies of a wild-type strain (Berlin K) that were 1-3 days old at the beginning of treatment. The treated males were mated with 3 virgin females of the Base strain and subsequently re-mated every 2 or 3 days with 3 fresh virgins up to a maximum of 5 such mating periods (broods). After short-term exposure, this mating procedure allows the separate sampling of germ-cell populations treated at different stages of their development. Upon long-term exposure, however, one may expect accumulation of damage according to the specific sensitivities of the stages through which the cells have proceeded during treatment. For the detection of SLRL mutations, the standard scheme was performed.
DBM is not mutagenic when evaluated in the Drosophila melanogaster sex-linked recessive lethal test.
The results given at the table below demonstrate the number of mortalities per number of chromosomes test in each mating period (brood) and in total.
Frequencies of sex-linked recessive mortalities after treatment with DBM
|
Expose. Time |
Conc. (mg/m3) |
Brood A nl/nchr# %l |
Brood B nl/nchr %l |
Brood C nl/nchr %l |
Brood D nl/nchr %l |
Brood E nl/nchr %l |
Brood A-E nl/nchr %l |
DBM |
6h |
60.2 |
0/396 0 |
1/386 0.26 |
1/396 0.25 |
0/394 0 |
0/392 0 |
2/1964 0.1 |
#Number of lethal/number of chromosomes tested
- Reason / purpose for cross-reference:
- exposure-related information
Reference
- Endpoint:
- genetic toxicity in vivo, other
- Remarks:
- DNA binding study in mammalians
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Reason / purpose for cross-reference:
- exposure-related information
- Reason / purpose for cross-reference:
- exposure-related information
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Rats and mice were treated i.p. with (14)C-dichloromethane, -dibromomethane, -1,2-dichloroethane, or -1,2-dibromoethane [5 mg (kg body weight)(-1)], and livers and kidneys were collected to rapidly isolate DNA. The DNA was digested using a procedure designed to minimize processing time, because some of the potential dihalomethane-derived DNA-glutathione (GSH) adducts are known to be unstable, and the HPLC fractions corresponding to major adduct standards were separated and analyzed for (14)C using accelerator mass spectrometry
- GLP compliance:
- not specified
- Species:
- rat
- Strain:
- Fischer 344
- Details on species / strain selection:
- also tested in mice strain B6C3F1
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- 2 males/dose/timepoint; Mice: 2/sex/timepoint; 24 control animals used but number/species and strain not stated.
- Route of administration:
- intraperitoneal
- Vehicle:
- Phosphate buffered saline
- Frequency of treatment:
- Once.
- Post exposure period:
- Animals sacrificed at 1 and 8 hrs after administration (method of sacrifice not stated). Kidneys and livers were removed with approximately 100 mg of each used for DNA
isolation and analysis. - Dose / conc.:
- 5 other: mg/kg/bw
- Remarks:
- (= 69 μCi/kg bw)
- No. of animals per sex per dose:
- Rats: 2 males/dose/timepoint; Mice: 2/sex/timepoint; 24 control animals used but number/species and strain not stated.
- Control animals:
- yes
- Tissues and cell types examined:
- Kidneys and livers
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- not specified
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Conclusions:
- None of four known (in vitro) GSH-DNA adducts was detected at a level of >2/10(8) - detection limit -DNA bases from dibromomethane
Data source
Reference
- Reference Type:
- publication
- Title:
- HPV1 TEST RULE STUDY – DATA ADEQUACY ASSESSMENTSRC TR-10-066 HPV1 TEST RULE STUDY – DATA ADEQUACY ASSESSMENT - Dibromomethane, CAS No. 74-95-3
- Author:
- Environmental Science Center
SRC, Inc - Year:
- 2 010
- Bibliographic source:
- https://chemview.epa.gov/chemview/
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dibromomethane
- EC Number:
- 200-824-2
- EC Name:
- Dibromomethane
- Cas Number:
- 74-95-3
- Molecular formula:
- CH2Br2
- IUPAC Name:
- dibromomethane
1
Method
Species / strain
- Species / strain / cell type:
- other: TA97,TA98,TA100,TA104
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- 0,10,25,50,100,250,500,1000 µg/plate
- Vehicle / solvent:
- DMSA
Controls
- Untreated negative controls:
- yes
- Positive controls:
- yes
Results and discussion
Test resultsopen allclose all
- Species / strain:
- other: TA97,TA98,TA100,TA104
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- other: TA98,TA100,TA104
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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