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EC number: 305-203-0 | CAS number: 94349-84-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 72 hours
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- Guideline study to GLP
The study performed on hop extract is relevant for iso-alpha acids, since iso-alpha acids are derived from hop extract. A further study has been planned for iso-alpha acids and will be entered as an update. - Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch no. 160025
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Strain CCAP 278/4
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 20.7 - 26.0 oC
- pH:
- 7.53 - 8.08
- Reference substance (positive control):
- yes
- Remarks:
- Separate GLP rerefence study on potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 86.7 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 83.1 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 25 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 42.7 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 52.5 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 12.5 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- yes
- Conclusions:
- Notes from report:
The definitive test conducted from 06 to 09 March 2018 was performed according to the OECD 201 (2011) guideline.
The growth curves demonstrate that the algae in the control were in logarithmic growth for the duration of the study.
The 48 hour EC(r)50 and EC(y)50 of HOP EXTRACT to Pseudokirchneriella subcapitata were 86.7mg/l and 42.7mg/l respectively (both determined by Maximum Likelihood-Logit).
The 72 hour EC(r)50 and EC(y)50 of HOP EXTRACT to Pseudokirchneriella subcapitata were
83.1mg/l and 52.4mg/l respectively (both determined by Maximum Likelihood-Logit).
The 0 to 72-hour NOEC(r) was 25mg/l and the 0-72 hour LOEC was 50mg/l (both determined by direct observation).
The 0 to 72-hour NOEC(y) was 12.5mg/l and the 0-72 hour LOEC was 25mg/l (both determined by direct observation).
The algal cells were examined microscopically during the determination of cell concentration.
All cells within the control and test concentrations appeared normal, no abnormalities were observed
(see section 3.2).
All validity criteria for the definitive test were met (see section 3.5).
The water quality measurements and incubation conditions are given in the summary tables in
Appendix 2 and were within accepted limits.
Analytical measurements of the freshly prepared exposure solutions indicated that the initial measured concentrations were not within 20% of nominal concentrations. In addition, analytical measurements of the 48 hour aged exposure solutions could not detect the test substance in the samples and therefore were also not within the ± 20% of nominal concentrations or ± 20% of the initial measured concentrations. This was expected due to the lack of water-solubility of HOP EXTRACT. All effect loading rates of the test substance are reported as nominal concentrations of the test substance. - Executive summary:
The study performed on hop extract is relevant for iso-alpha acids, since iso-alpha acids are derived from hop extract. A further study has been planned for iso-alpha acids and will be entered as an update.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 72 hours
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch no. DB EXP 9
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Strain CCAP 278/4
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 21 - 21.6 oC
- pH:
- 7.56 – 9.12
- Reference substance (positive control):
- yes
- Remarks:
- Separate GLP rerefence study on potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- yes
- Conclusions:
- The definitive test conducted from 21 to 24 August 2018 was performed according to the OECD 201 (2011) guideline.
The growth curves illustrated in Graph 1 demonstrate that the algae in the control were in logarithmic growth for the duration of the study.
The 72 hour EC(r)50 and EC(y)50 of Potassium salt of Iso-alpha acids to Pseudokirchneriella subcapitata were >100 mg/l and >100 mg/l respectively (determined by Linear Interpolation).
The 0 to 72-hour NOEC(r) was >100 mg/l and the 0-72 hour LOEC was >100 mg/l (determined by direct observation).
The algal cells were examined microscopically during the determination of cell concentration. All cells within the control and test concentrations appeared normal, no abnormalities were observed (see section 3.2).
All validity criteria for the definitive test were met (see section 3.5).
The water quality measurements and incubation conditions are given in the summary tables in Appendix 2 and were within accepted limits.
Results for the analytical confirmation of exposure concentrations are shown in Appendix 3. The results indicate that the measured concentrations remained within 80-120% of nominal (81.344%). Therefore effect concentrations are reported as nominal concentrations of Potassium salt of Iso-alpha acids as received.
Referenceopen allclose all
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.