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EC number: 240-941-6 | CAS number: 16898-52-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 September 2017 to 08 November 2017
- Reliability:
- 1 (reliable without restriction)
- Justification for type of information:
- Reliable experimental study following OECD 201 guidelines (2006) and EC No. 761/2009 Method C.3 adopted 2009 and GLP standards.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 2006
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- 761/2009
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- The test substance is listed as DIPIP (1,3-Di-4-Piperidylpropane). The test substance consists of white flakes. The test substance was obtained from the Sponsor, Vertellus Holdings LLC and stored at room temperature in the dark.
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test was carried out using Pseudokirchneriella subcapitata strain CCAP 278/4. Liquid cultures of Pseudokirchneriella subcapitata were obtained from the Culture Collection of Algae and Protozoa (CCAP), SAMS Research Services Ltd, Scottish Marine Institute, Oban, Argyll, Scotland. Master cultures were maintained in the laboratory by the periodic replenishment of culture medium. The master cultures were maintained in the laboratory under constant agitation by orbital shaker (100 to 150 rpm) and constant illumination at 24 ±1 °C.
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
- Test temperature:
- 24 °C ± 1 °C
- pH:
- Control cultures pH range 7.2 – 8.0; Test cultures had a concentration dependant increase in pH at 0 hours with a pH of 7.3 at 1.0 mg/L through to pH 10.9 at 100 mg/L. This was considered to be due to an intrinsic property of the test item and as such no adjustment to the pH of the test solutions was made prior to the addition of the algal cells.
- Nominal and measured concentrations:
- The green alga was exposed to an aqueous solution of the test item at nominal concentrations of 1.0 mg/L, 3.2 mg/L, 10 mg/L, 32mg/L and 100 mg/L (three replicate flasks per concentration).
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate was used as the positive control reference item.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Results with reference substance (positive control):
- A positive control (Envigo Study Number RD71YQ) used potassium dichromate as the reference item at concentrations of 0.25 mg/L, 0.50 mg/L, 1.0mg/L, 2.0 mg/L and 4.0 mg/L. The results from the positive control with potassium dichromate were within the normal ranges for this reference item.
- Validity criteria fulfilled:
- yes
- Remarks:
- Cell conc. control increased by factor of 250 at 72 h (protocol ≥ factor of 16); mean coefficients of variation growth rates 10% (protocol ≤ 35%); coefficient of variation avg. specific growth rate in replicate control cultures 1% (protocol ≤ 7%).
- Conclusions:
- The test substance caused a reduction of growth in a series of algal cultures (test units) exposed to the test item for 72 h. The 72 h EC50 was 22 mg/L and the 72 h LOEC was 32 mg/L as determined by comparison of areas in growth curves. The 72 h NOEC was 10 mg/L as determined by comparison of areas in growth curves.
Reference
Description of key information
The key study was a static acute toxicity test on the effects of the substance on the freshwater unicellular green alga, Pseudokirchneriella subcapitata following OECD Guideline 201. The test substance caused a reduction of growth in a series of algal cultures (test units) exposed to the test item for 72 h. The 72 h ErC50 was 22 mg/L and the 72 h LOErC was 32 mg/L as determined by comparison of areas in growth curves. The 72 h NOErC was 10 mg/L as determined by comparison of areas in growth curves.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 22 mg/L
- EC10 or NOEC for freshwater algae:
- 10 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.