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EC number: 638-734-4 | CAS number: 157248-24-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-06-22 to 2017-06-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- July, 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test item was weighted directly in Erlenmeyer flasks and stirred overnight with test medium.
- Controls: negative control: test medium without substance addition; positive control: reference substance Copper (II) sulphate pentahydrate p.a. (concentrations 58, 100 and 180 mg/L)
- Test concentration separation factor: A limit test was performed.
- Evidence of undissolved material: No - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: not applicable
- Name and location of sewage treatment plant where inoculum was collected: Municipal sewage treatment plant of 31137 Hildesheim, Germany
- Method of cultivation: A fresh sample was used.
- Pretreatment: The sludge was washed twice with chlorine free tap water and adjusted to a dry sludge concentration of 3.0 g/L ± 10 %. The sludge was used within 24 h after sampling.
- Initial biomass concentration: Dry sludge concentration was 2.94 g/L (corresponding to 1.47 g/L suspended solids in the test vessels). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- not applicable
- Hardness:
- not specified
- Test temperature:
- 20.9 °C
- pH:
- activated sludge: 7.60
synthetic waste water: 7.18 - Dissolved oxygen:
- determined at start of measurement:
control: 7.13 - 9.56 mg O2/L
reference item: 5.72 - 8.02 mg O2/L
test item: 6.85, 7.14, and 6.72 mg O2/L - Salinity:
- not applicable
- Conductivity:
- not specified
- Nominal and measured concentrations:
- 1000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL Erlenmeyer flasks, DIN 12380
- Type: open
- Material, headspace, fill volume: glass, not specified, 500 mL
- Aeration: Permanent, to keep the dissolved oxygen concentration above 60 - 70 % saturation and to maintain the sludge flakes in suspension.
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Sludge concentration (weight of dry solids per volume): 2.94 g/L (corresponding to 1.47 g/L suspended solids in the test vessels)
- Nutrients provided for bacteria: Synthetic wastewater according to OECD-Guideline 209 was used as nutrient solution.
- Nitrification inhibitor used: none
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic waste water (16 mL) according to OECD-Guideline 209 and Demineralised water (to fill up to 250 mL after addition of test or reference item), together with Inoculum (250 mL) were used in preparation of test/reference item solution.
OTHER TEST CONDITIONS
- Adjustment of pH: not performed
- Photoperiod: not specified
- Light intensity: not specified
- Details on termination of incubation: not specified
EFFECT PARAMETERS MEASURED: The oxygen concentration in the measurement chamber was measured by the oximeter by optical read-out of the sensor spots at least every 5 seconds.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Limit test was performed.
Range finding study
- Test concentrations: 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: Based on the results of the preliminary test it could concluded that the heterotrophic respiration as well as the nitrification are not inhibited. Therefore, the definitive study was carried out as a limit test using 1000 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate p.a.
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: No inhibiting effects were observed up to the limit concentration.
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No such effects were observed.
- Effect concentrations exceeding solubility of substance in test medium: No.
- Adsorption (e.g. of test material to the walls of the test container): No.
- Blank controls oxygen uptake rate: 35.4 +/- 1.28 mg O2/(L*h)
- Coefficient of variation of oxygen uptake rate in control replicates: 3.62 % - Results with reference substance (positive control):
- - Results with reference substance valid: Yes.
- Relevant effect levels: EC50 = 96 mg/L - Reported statistics and error estimates:
- The NOEC was determined by calculation of statistical significance of the inhibition of respiration in comparison to the control. One Way Analysis of Variance (ANOVA) was used for NOEC calculation. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. P-value for both tests was 0.05. The α-value (acceptable probability of incorrectly concluding that there is a difference) is α=0.05. No statistically significant inhibiting effect was determined for the test item (P = 0.063), ANOVA.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test material caused no inhibiting effects on activated sludge of a municipal sewage treatment plant at the limit test item concentration of 1000 mg/L and the NOEC was determined to be ≥ 1000 mg/L.
- Executive summary:
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 (2010) was carried out with the test item. The test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany. The test was carried out under static conditions with the limit test item concentration 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates was 6%. In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 96.0 mg/L. The validity criteria of the test guideline were fulfilled. The test material caused no inhibiting effects on activated sludge of a municipal sewage treatment plant at the limit test item concentration of 1000 mg/L and the NOEC was determined to be ≥ 1000 mg/L.
Reference
Description of key information
The test material caused no inhibiting effects on activated sludge of a municipal sewage treatment plant at the limit test item concentration of 1000 mg/L and the NOEC was determined to be ≥ 1000 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 (2010) was carried out with the test item. The test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany. The test was carried out under static conditions with the limit test item concentration 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates was 6 %. In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 96.0 mg/L. The validity criteria of the test guideline were fulfilled. The test material caused no inhibiting effects on activated sludge of a municipal sewage treatment plant at the limit test item concentration of 1000 mg/L and the NOEC was determined to be ≥ 1000 mg/L.
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