Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 210-279-2 | CAS number: 611-74-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- single dose application, counting deaths after 48 hours
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
Data source
Reference
- Reference Type:
- review article or handbook
- Title:
- Unnamed
- Year:
- 1 971
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The compounds were reduced to fine powder, suspended in 1% methylcellulose mucilage and homogenized. The suspension was administered orally to groups of 20 mice using a dose volume of 0.5 ml/20g bw. Deaths were counted after 48h and the LD50 limits were calculated.
Analgesic properties of the compounds were detected by hotplate and writhing test. In the hotplate test, 20 mice were used at each of the 4 dose levels. Every 30 min for 3 hours the mice were placed at a hotplate (55 +/- 0.5 °C) for 20 sec. The flicking of the hind paws were counted. 20 mice given 1% methylcellulose only were used as control.
For the writhing test 12 mice were dosed orally with the test compound. 30 min. later formic acid (25 mg/kg bw, ip) was injected. The animals were placed individually in observation chambers and the number of writhing episodes within the next 20 min. was counted. A group of 12 mice, treated with methylcellulose only served as control.
ED50 (hotplate or writhing) were calculated by standard statistical methods. - GLP compliance:
- no
- Test type:
- other:
- Limit test:
- no
Test material
- Reference substance name:
- N,N-dimethylbenzamide
- EC Number:
- 210-279-2
- EC Name:
- N,N-dimethylbenzamide
- Cas Number:
- 611-74-5
- Molecular formula:
- C9H11NO
- IUPAC Name:
- N,N-dimethylbenzamide
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- Amides were prepared by standard methods from the appropriate amines and acids, obtained commercially or prepared by published methods.
Test animals
- Species:
- mouse
- Strain:
- other: albinos from the QS strain
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- groups of 20 female mice, weighing 18-22 g for LD50 test
4 groups of 20 female mice, weighting 18-22 g for the hotplate test, another group of 20 mice served as control
4 (?) groups of 12 female mice, weighting 18-22 g for the writhing test, another group of 12 mice served as control
Administration / exposure
- Route of administration:
- oral: unspecified
- Details on oral exposure:
- The compounds were reduced to fine powder, suspended in 1% methylcellulose mucilage and the mixture was homogenized. The suspension was administered orally to groups of mice using a dose volume of 0.5 ml/ 20 g bw.
- Doses:
- not specified in the article
1 dose level for the LD50 test
4 dose levels for the hotplate test
some dose levels for the writhing test - No. of animals per sex per dose:
- 20 females for LD50 test
20 femals per dose for the hotplate test + 20 female for the control
12 females per dose for the writhing test + 12 females for the control - Control animals:
- other:
- Remarks:
- none for LD50, 20 for hotplate, 12 for writhing
- Details on study design:
- LD50: oral application in 20 mice. Deaths were counted after 48 hours
Analgesic properties: Hotplate test and writhing test - Statistics:
- LD50, ED50 (hotplate) and ED50 (writhing) including confidence limits were calculated by the method of Litchfield and Wilcoxon (1949).
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- ca. 960 mg/kg bw
- Based on:
- not specified
- Remarks on result:
- other: 807-1142 mg/kg bw
- Remarks:
- limits of error for P = 0.05
- Sex:
- female
- Dose descriptor:
- other: ED50
- Remarks:
- hotplate
- Effect level:
- ca. 250 mg/kg bw
- Based on:
- not specified
- Sex:
- female
- Dose descriptor:
- other: ED50
- Remarks:
- writhing
- Effect level:
- ca. 211 mg/kg bw
- Based on:
- not specified
- Mortality:
- not specified
- Clinical signs:
- analgetic properties
depressant activities at high doses, ataxia, cyanosis of ears, tail and feet - Body weight:
- 18-22 g at the beginning of the test
- Gross pathology:
- not mentioned
- Other findings:
- analgesic properties
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- According to this publiction the test substance has a LD50 = 960 mg/kg bw for female mice. It also has analgesic properties.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.