Cyclohexane-1,2,4-triyltris(ethylene)

Ecotoxicological Summary

Hazard for aquatic organisms

Freshwater

Hazard assessment conclusion:

PNEC aqua (freshwater)

PNEC value:

0 mg/L

Assessment factor:

100

Extrapolation method:

assessment factor

PNEC freshwater (intermittent releases):

0.007 mg/L

Marine water

Hazard assessment conclusion:

PNEC aqua (marine water)

PNEC value:

0 mg/L

Assessment factor:

1 000

Extrapolation method:

assessment factor

STP

Hazard assessment conclusion:

PNEC STP

PNEC value:

75.4 mg/L

Assessment factor:

10

Sediment (freshwater)

Hazard assessment conclusion:

PNEC sediment (freshwater)

PNEC value:

1.36 mg/kg sediment dw

Extrapolation method:

equilibrium partitioning method

Sediment (marine water)

Hazard assessment conclusion:

PNEC sediment (marine water)

PNEC value:

0.136 mg/kg sediment dw

Extrapolation method:

equilibrium partitioning method

Hazard for air

Air

Hazard assessment conclusion:

no hazard identified

Hazard for terrestrial organisms

Soil

Hazard assessment conclusion:

PNEC soil

PNEC value:

0.482 mg/kg soil dw

Extrapolation method:

equilibrium partitioning method

Hazard for predators

Secondary poisoning

Hazard assessment conclusion:

insufficient hazard data available (further information necessary)

Additional information

Studies with 1,2,4 -Trivinylcyclohexane are available for the trophic levels fish, daphnia, algae and aquatic microorganisms. In the acute toxicity tests, toxic effects in fish were observed. The EC50 for Daphnia magna has been determined >15.4 mg/L and in an algae toxicity test an ErC50 and an ErC10 >1.98 mg/L have been measured. Although, methodological limitations, the acute fish test has been used to derive the PNECintermittend release in a worst case approach. The PNEC freshwater has been derived based on the results of the chronic study with invertebrates applying an assessment factor of 100 because the value is the lowest of the two available long-term results covering two trophic levels (algae and invertebrates) in accordance to the ECHA guidance document R.10 (2008). The calculation of the PNEC for STP has been based on the available oxygen consumption test with P. putida, in which an EC10 of 754 mg/L has been measured. The assessment factor of 10 has been chosen as the test procedure is comparable to a respiration inhibition test.

The PNEC sediment, PNEC sediment marine and the PNEC soil have been derived using the equilibrium partitioning method using the KOC and the Henry’s law constant of the test substance and the PNEC freshwater or PNEC marine water. Further calculation factors, like the conversion factor from wet to dry, were chosen according to the guidance document R. 10 (May 2008). A PNEC air has not been derived as no hazard has been identified. The PNEC for secondary poisoning was not derived due to limited data. However, the test substance is not classified as CMR or STOT RE1 or RE2. Hence a detailed assessment has not been conducted.

Conclusion on classification

Under the tested conditions, 1,2,4-Trivinylcyclohexane has not been observed to be readily biodegradable. Acute studies are available for the trophic levels fish, daphnia, algae and aquatic microorganisms. In an acute toxicity test with zebrafish, a LC50 of 3.2 mg/L has been observed and in a second fish study with substance specific analytic, a LC50 of 0.652 mg/L has been determined. Both results are above the water solubility limit of the test substance. Nevertheless, the toxic effects should be considered for classification and labeling. In a long-term study on daphnia, a NOEC (21 d) for reproduction of 0.0444 mg/L and an EC10 (21 d) for reproduction of 0.0414 mg/L have been determined. A long-term test with fish is not available, in the toxicity test with algae an ErC10 > 1.98mg/L has been observed.

Based on the acute toxicity data, the test substance should be classified as acute toxic for the aquatic compartment, Category 1 (H400) according to CLP regulation (M-factor 1). Based on the chronic data of the study with invertebrates and based on the acute data for fish, a classification as aquatic chronic Category 1 (H410) is required (M-factor 1). The classification according to UN-GHS regulation would result in the same classification.