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EC number: 211-541-9 | CAS number: 660-68-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to the attached analogue justification provided in IUCLID Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.2 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- - Mortality of control: 0% at the end of the exposure
- Other adverse effects control: no adverse effects were observed in the control group - Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 June - 11 July 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- 1998
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- according to the GLP standard by the Japanese Ministry of Environment
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: in the dark at the room temperature
- Stability under test conditions: verified at the beginning of and at the end of the exposure by IR, NMR and HPLC - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All concentrations of test solution were analyzed at Day 0 (new), 2 (old), 8 (new), 10 (old), 15 (new), and 17 (old).
- Sampling method: directly sampled - Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Age of parental stock: between 0 - 24 h
- Feeding during test: yes
- Food type: Chlorella vulugaris
- Amount: 0.1 - 0.2 mg C/d
ACCLIMATION
- Acclimation period: 4 weeks
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Chlorella vulugaris, 0.1 - 0.2 mg C/d - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 249 - 268 mg/L (as CaCO3)
- Test temperature:
- 19.9 - 20.9 °C
- pH:
- 6.7 - 9.9
- Dissolved oxygen:
- 7.9 - 8.9 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: control, 2.7, 4.8, 8.6, 15.4, 27.8, and 50.0 mg/L
Time-weighted mean measured concentrations:- Details on test conditions:
- TEST SYSTEM
- Material, size: Glass, 100 mL
- Renewal rate of test solution: 3 times a week
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
OTHER TEST CONDITIONS
- Photoperiod: 16/8 (light/dark)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
mortality of parental Daphnia, cumulative numbers of dead parental Daphnia, time to first brood production and mean cumulative numbers of juveniles produced per adult
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study: an acute toxicity tes for 48 h was performed
- Test concentrations: not available in the report
- Results used to determine the conditions for the definitive study: EiC50 for 48 h was 58.4 mg/L- Reference substance (positive control):
- no
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.2 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- - Mortality of control: 0% at the end of the exposure
- Other adverse effects control: no adverse effects were observed in the control group- Reported statistics and error estimates:
- EcoTox-Statics (Version 1.1) was used ti calculate EC50, LC50 and NOEC
ANALYTICAL RESULTS:
The pH of new solutions of higher concentrations was high and pH change during the exposure was cuased by the test substance. However, the test condition in terms of water temperature, dissolved oxygen concentration, pH and water hardness was appropriate for the test organism.
Table 1: pH during the 21 -d test
pH Measured concentrations [mg/L] 0 d
new2 d
old8 d
new10 d
old15 d
new17 d
oldcontrol 7.2 7 7.2 6.7 7.4 7 2.2 7.3 7 7.6 6.8 7.6 7.3 4.2 7.6 7.1 7.9 6.9 7.9 7.3 7.7 8 7.1 8.4 6.9 8.2 7.4 14.7 8.5 7.2 9 7 8.7 7.5 26.9 9.1 7.3 9.4 7.1 9.2 7.6 49.2 9.8 7.5 9.9 7.2 9.8 7.8 Table 2: Time-weighted means of measured concentrations
Nominal concentration
(mg/L)
Time-weighted Mean
(mg/L)
Percent of nominal
(%)
Control
-
-
2.7
2.2
81.5
4.8
4.2
87.5
8.6
7.7
89.5
15.4
14.7
95.5
27.8
26.9
96.8
50.0
49.2
98.4
BIOLOGICAL RESULTS:
Time to first brood production was 8 - 11 days in the control group. Although it was 10 - 14 days in the 2.2 mg/L, no brood was observed at 26.9 and 49.2 mg/L.
Table 3: Cumulative numbers of juveniles produced per adult alive for 21 days
Control
Measured concentration (mg/L)
2.2
4.2
7.7
14.7
26.9
49.2
Mean
86.5
75.4
76.4
17.8
2.8
0.0
0.0
S.D.
38.67
29.76
40.58
11.67
5.60
0.0
0.0
Inhibition rate (%)
12.8
11.7
79.4
96.8
100.0
100.0
Significant difference*1
**
**
**
**
*1: indicates a significant difference by Dunnet multiple comparison procedure, Two-sided test
**: indicates a significant difference (α = 0.001) from the control
Table 4: Mortality (%) of parental Daphnia
Measured concentration
(mg/L)
Days
1
2
4
7
14
21
Control
0
0
0
0
0
0
2.2
0
0
0
0
0
0
4.2
0
0
0
0
10
10
7.7
0
0
0
0
10
40
14.7
0
0
0
0
0
40
26.9
0
0
0
0
0
20
49.2
0
0
0
0
0
20
Other effect concentrations:
LC50 (21 d) > 49.2 mg/L (meas. TWA)
LOEC (21 d) = 7.7 mg/L (meas. TWA)
ErC50 (21 d) = 5.7 mg/L (meas. TWA)
VALIDITY CRITERIA:
Since the mortality of the parent animals (female Daphnia) did not exceed 20% at the end of the test and the mean number of living offspring produced per parent animal surviving at the end of the test was > 60, the test was considered to be valid.
- Validity criteria fulfilled:
- yes
Referenceopen allclose all
Description of key information
NOEC (21 d) = 4.2 mg/L (meas. TWA) based on read across from CAS 109-89-7
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 4.2 mg/L
Additional information
Experimental data on the long term toxicity of Diethylammonium chloride (CAS 660-68-4) towards aquatic invertebrates are not available. Therefore appropriate read across data from the analogues substance N-ethylethanamine (CAS 109-89-7) were used for the environmental assessment.
The toxicity of the read across substance towards the test organism Daphnia magna was tested according to the OECD guideline 211 and GLP standards. Semi-static conditions were used for the test with a renewal rate of 3 times per week. The test organism was exposed for 21 d to nominal test item concentrations of 2.7, 4.8, 8.6, 15.4, 27.8, 50.0 mg/L (corresponding to measured: 2.2, 4.2, 7.7, 14.7, 26.9, and 49.2 mg/L) and a control. The presence of the tested substance in the testing medium, was analytically monitored by HPLC. The recoveries were between 81.5 and 98.4%. The test resulted in a NOEC (21 d) of 4.2 mg/L (meas., TWA).
Based on the results of the read across substance, similar effects are expected by the target substance Diethylammonium chloride (CAS 660-68-4) towards aquatic invertebrates.
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