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Diss Factsheets
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EC number: 278-133-0 | CAS number: 75214-65-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Acid Black 222
- IUPAC Name:
- Acid Black 222
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: KFM, CH-4414 Füllinsdorf, Switzerland.
- Age at study initiation: young adult male and female.
- Weight at study initiation: ranged between 150 and 190 g.
- Fasting period before study: access of food only was prevented approximately 18 hours prior and four hours after the dosing.
- Housing: the rats were housed individually in Macrolon cages.
- Diet: standard laboratory pelleted diet (KLIBA no. 24-343-4 from Klingentalmühle AG., Basle), ad libitum. The batch of diet used was analysed for chemical and microbiological contaminants.
- Water: ad libitum. The water bottles were withdrawn two hours prior and four hours after dosing.
- Acclimation period: about five days.
ENVIRONMENTAL CONDITIONS
- Temperature: 23 ± 2 °C
- Humidity: 30 - 70 %
- Air changes: approximately 15 changes/hour.
- Photoperiod: 12 hrs dark / 12 hrs light.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- - Dosed volume was 20 ml/kg bw
- Dilution: test item was diluted in 25 % water. - Doses:
- 5000 mg/kg
- No. of animals per sex per dose:
- five male and five female
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations:animals were observed soon after dosing, then at hourly intervals for the remainder day 1. On the subsequent days the animals were observed once in the morning and once in the Iate afternoon. Clinical signs were recorded at each observation. The following was recorded: approximate time of death; the nature, severity, approximate time of onset and duration of each toxic sign; individual body weights on day 1, 7 and 14.
- Necropsy of survivors performed: yes; surviving animals were killed after two weeks. All animals which died during the study and those killed after two weeks were subjected to a macroscopic postmortem examination. Macroscopic appearance of abnormal organs was recorded.
PRELIMINARY TEST
A preliminary study was carried out to establish a dosing regimen, using groups of two males and two females at one dosage, using 20 ml/kg bw.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- not determinable due to absence of adverse toxic effects
- Mortality:
- No deaths occurred.
- Clinical signs:
- The animals were flaccid, weak and showed rough coat, decreased movement and diarrhea.
- Gross pathology:
- No special findings.
Applicant's summary and conclusion
- Interpretation of results:
- other: not classified, according to the CLP Regulation (EC) No 1272/2008
- Conclusions:
- LD50 > 5000 mg/kg bw (males and females)
- Executive summary:
Ten rats were treated at a dosage level of 5000 mg/kg bw. The substance was administered as a single dose by gavage. After administration of the compound, animals were observed for 14 days; at the end of the observation period, surviving animals were killed and an autopsy performed.
No deaths occurred during the 14 day observation period; animals appeared flaccid, weak and showed rough coat, decreased movement and diarrhea. At autopsy no special findings were seen.
Conclusion
LD50 > 5000 mg/kg bw (males and females)
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