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EC number: 240-387-5 | CAS number: 16298-03-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 April 2013 - 25 April 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- Principles of method if other than guideline:
- none
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methyl 3-aminopyrazinecarboxylate
- EC Number:
- 240-387-5
- EC Name:
- Methyl 3-aminopyrazinecarboxylate
- Cas Number:
- 16298-03-6
- Molecular formula:
- C6H7N3O2
- IUPAC Name:
- methyl 3-aminopyrazine-2-carboxylate
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Name of test material (as cited in study report): Amiloride Compound 6
- Description: Tan powder
- Storage condition of test material: In refrigerator (2-8°C) protected from light, desiccated
- Other: Reactive to light, moisture and oxygen
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Singular samples for possible analysis were taken from all test concentrations and the control according to the schedule below.
Frequency: at t=0 h and t=48 h
Volume: 2 ml from the approximate centre of the test vessels
Storage: Samples were stored in a freezer until analysis.
At the end of the exposure period, the replicates were pooled at each concentration before sampling.
Additionally, singular reserve samples of 2 ml were taken for possible analysis. If not used, these samples were stored in a freezer.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The batch of Amiloride Compound 6 tested was a tan powder with a purity of 100% based on area percentage and completely soluble in test medium at the concentrations tested.
Preparation of test solutions started with individual loading rates ranging from 1.0 to 100 mg/l. A 30 and a 90-minute period of magnetic stirring were applied to accelerate the dissolving of the test substance in the medium, in the combined limit/range-finding test and the final, respectively. The final test solutions besides the highest test concentration were clear and colourless. The highest test concentration was clear and slightly yellow.
Note that due to the light sensitivity of the test substance all handlings were performed under dimmed light and glassware was either amber coloured or covered in aluminium foil.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna (Crustacea, Cladocera) (Straus 1820), at least third generation, obtained by acyclical parthenogenesis under specified breeding conditions.
Source: In-house laboratory culture with a known history
Reason for selection: This system has been selected as an internationally accepted invertebrate species.
Validity of the batch: Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood.
Characteristics: For the test selection of young daphnids with an age of < 24 hours, from parental daphnids of more than two weeks old.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- no
Test conditions
- Hardness:
- 180 mg/l expressed as CaCO3
- Test temperature:
- 18.8 - 21.0 °C
- pH:
- 7.8-8.1
- Dissolved oxygen:
- 8.9-9.4 mg/l
- Salinity:
- not measured
- Nominal and measured concentrations:
- nominal concentrations: 10, 18, 32, 56 and 100 mg/l.
The actual concentrations, measured in the solutions containing 32, 56 and 100 mg/l, were at the level of nominal (100-104% of nominal) and remained stable during the test (91-93% of initial). Therefore, the effects parameters were reported in terms of analytically confirmed nominal concentrations - Details on test conditions:
- Test duration: 48 hours
Test type: Static
Test vessel: 100 ml, all glass
Medium: Adjusted ISO-medium
Number of daphnids: 20 per group
Loading: 5 per vessel containing 80 ml of test solution
Light: the test was performed under dimmed light conditions
Feeding: No feeding
Aeration: No aeration of the test solutions
Introduction of the daphnids: within 18 minutes after preparation of the test solutions. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 73 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- analytically confirmed nominal
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 65-87 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 68 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- analytically confirmed nominal
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 61-78 mg/L
- Details on results:
- - Behavioural abnormalities: no
- Other biological observations: none
- Immobility of control: no immobility was observed.
- Other adverse effects control: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- The 48h-EC50 was 0.53 mg/l with a 95% confidence interval between 0.49 and 0.62 mg/l. - Reported statistics and error estimates:
- The EC50-value was calculated at 24 and 48 hours of exposure from the probits of the percentages of affected daphnids and the logarithms of the corresponding test substance concentrations using the maximum likelihood estimation method (Finney, D.J., 1971: Probit analysis, Cambridge University Press, Cambridge, U.K., 3rd edition).
Any other information on results incl. tables
none
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Amiloride Compound 6 did not induce acute immobilisation of Daphnia magna at an analytical confirmed nominal concentration of 32 mg/l after 48 hours of exposure. At 48 hours the EC50 was reported as 68 mg/l (95% CL: 61-78 mg/L)(nominal)
The 48h-EC50 was 68 mg/l based on analytically confirmed nominal concentrations (95% confidence interval between 61 and 78 mg/l).
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