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EC number: 236-599-2 | CAS number: 13446-49-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Reported nominal toxic concentration levels were substance-based instead, i.e., not expressed as the Mo-ion, bus as the salt. Test was conducted according to OECD Guideline 209. Relevant endpoints were reported, or could be derived with the reported raw data.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
Test material
- Reference substance name:
- Molybdenum trioxide
- EC Number:
- 215-204-7
- EC Name:
- Molybdenum trioxide
- Details on test material:
- - Name of test material (as cited in study report): molybdenum oxide (pure)
- Physical state: light grey powder
- Analytical purity: 66.63% molybdenum
- Expiration date of the lot/batch: 13 June 1993
- Storage condition of test material: room temperature in the dark
- Method of preparation: direct dispersion in water
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Type and source of the inoculum was a mixed population of activated sewage sludge micro-organisms, taken from the aeration stage of the HRC Ltd. Sewage treatment plant treating predominantly domestic sewage. The collected sample was maintained at 20 ± 2 degrees Celsius with continuous aeration and used on the day of collection. pH was 7.4 and the suspended solid level of the sample was 4.1 g/L.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- also results after 30 min
Test conditions
- Hardness:
- dilution water: 150-200 mg/L as CaCO3
- Test temperature:
- 20 degrees Celsius
- pH:
- dilution water: 6.0-8.5
- Nominal and measured concentrations:
- 20, 60, 180, 540, 1620 mg/L of the substance
- Details on test conditions:
- - Contact time: 30 minutes and 3 hours
- Vessels: 500 mL conical flasks
- Aeration: compressed air via narrow bore glass tubes
- Measuring apparatus: 280 mL darkened glass BOD bottle fitted with a Yellow Springs BOD probe (Model 50B) and a magnetic stirrer. Separate measuring bottles were used for control, test and reference cultures to eliminate the possibility of cross-contamination.
- Photoperiod: normal laboratory lighting
- Control: inoculated medium without test or reference toxicant
- Test procedure: At time "0", 16 mL of synthetic sewage was made up to 300 mL with water in a 500 mL conical flask. 200 mL inoculum were added and the mixture aerated at approximately 0.5-1 L per minute. This preparation was designated Control R1. Thereafter, at 15 min intervals, further cultures were prepared following the same procedure but with the addition of appropriate amounts of test or reference substance to the vessel prior to adjusting the volume and adding the inoculum. The series was completed by a second Control R2 culture.
As each culture reached 30 minutes contact time its rate of respiration was measured. The measuring vessel was completely filled with the culture and sealed by the dissolved oxygen probe before being placed on a magnetic stirrer. THe stirrer was started and the dissolved oxygen level was recorded for approximately 10 minutes. At the end of the recording period the sample was returned to the culture vessel and aeration continued until the 3-hour contact period was reached. At this time, the determination of respiration rate was repeated.
Synthetic sewage composition: 16g peptone, 11g meat extract, 3g urea, 0.7g NaCl, 0.4g CaCl2.2H2O, 0.2g MgSO4.7H2O, 2.8g K2HPO4 dissolved in 1L tap water
Dilution water: Laboratory tap water, dechlorinated by passage through activated carbon and softened by reverse osmosis. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol, 97% purity
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 1 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 820 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 325 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 253.7-416.2
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 216.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 169.1-277.3
- Details on results:
- The raw data for the test that was conducted with pure Mo-oxide allowed the derivation of an EC10-value. The use of this value is preferred, and reduces the uncertainty of a PNEC that may be derived using this specific study (i.e. an assessment factor of 10 on an EC10-value versus an assessment factor of 100 on an EC50-value). Fitting a log-logistic distribution through the reported raw data of the 3h-test with pure MoO3 resulted in a 3h-EC10 of 325.0 mg MoO3/L (95%CL: 253.7-416.2) or 216.5 mg Mo/L (95%CL: 169.1-277.3). It should be noted that this concentration level of molybdenum, added as MoO3, may strongly reduce the pH-level. As the pH during the exposure period was not reported, it is always possible that the observed effects are more pH-related than Mo-related.
Rate of respiration for each culture was measured for a 10 minute period
Variation in respiration rates of Controls R1 and R2: +/-1.7% after 30 min, and +/- 4.0% after 3h.
- Results with reference substance (positive control):
- 30 min-EC50: 20 mg/L
3h-EC50: 17 mg/L - Reported statistics and error estimates:
- Percentage inhibition plotted against concentration
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Reported nominal toxic concentration levels were substance-based instead, i.e., not expressed as the Mo-ion, bus as the salt. Method is well described and in line with OECD 209. EC10 level could be determined with the reported data . EC50-values are categorised as Klimisch 2 data points (reliable with restrictions), as pH may have had an influence on the reported effect levels.
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