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Diss Factsheets
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EC number: 909-092-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 Mar 2017 to 24 Mar 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of 5,12-dihydroquino[2,3-b]acridine-7,14-dione and aluminium tris(5,7,12,14-tetrahydro-7,14-dioxoquino[2,3-b]acridine-2-sulphonate) and dialuminium tris(5,7,12,14-tetrahydro-7,14-dioxoquino[2,3-b]acridine-2,9-disulphonate)
- EC Number:
- 909-092-5
- Molecular formula:
- Unspecified
- IUPAC Name:
- Reaction mass of 5,12-dihydroquino[2,3-b]acridine-7,14-dione and aluminium tris(5,7,12,14-tetrahydro-7,14-dioxoquino[2,3-b]acridine-2-sulphonate) and dialuminium tris(5,7,12,14-tetrahydro-7,14-dioxoquino[2,3-b]acridine-2,9-disulphonate)
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
The sample of the test item was stored at ambient temperature without exposure to light in a tightly sealed container.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The total organic carbon was determined in samples at exposure initiation, at renewal, and at exposure termination since the test substance is a multi-constituent. At exposure initiation and at renewal from test item concentration and the control, two samples were collected for chemical analysis (each in a volume of 100 mL). One was transferred to chemical analyses as a fresh sample (at exposure initiation and at renewal), the other was stored under test conditions and transferred for chemical analyses as 24-hour old sample (at renewal and at exposure termination).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION OF TEST SOLUTIONS
Appropriate amount of the test item was weighed in glass flask with ISO test water and mixed with appropriate volume of the ISO test water. The mixture of a loading of 100 mg/L was heterogeneous (with undissolved particles). The mixture and the control (800 mL ISO test water) were left for mechanical shaking for 48 hours (90 revolutions per minute, darkness). After 48 hours of mechanical shaking, the mixture was heterogeneous. The mixture and the control were filtrated, first through a conditioned paper filter (qualitative cellulose filter) and next through a conditioned nitrocellulose membrane filter (0.22 Xm, Millipore). Each filtrate was visually homogeneous (without any visible undissolved particles), clear, colourless.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: Originated from the standard laboratory culture cultivated at the Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology
CULTURING
Daphnia magna was cultured in glass beakers with a capacity of 150 mL (one parent per vessel) containing 100 mL of ISO test water at room temperature with daily cycle 16 h light : 8 h dark (KANLUX electronic time programmer, Poland). The culture was maintained in the ISO test water. The Daphnia magna were fed with a suspension of algae; mixture of two species Pseudokirchneriella subcapitata: Desmodesmus subspicatus (in 2:1 ratio) originating from separate cultures in the Laboratory of Aquatic Toxicology. Group B vitamins and micronutrients necessary for proper growth were supplied with the lyophilised suspension of Spirulina sp.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 240 mg/L as CaCO3
- Test temperature:
- 19 - 22 °C
- pH:
- - Test start: 7.58 - 7.69
- Test termination: 7.55 - 7.69 - Dissolved oxygen:
- - Test start: 8.9 - 9.9 mg/L
- Test termination: 8.7 - 9.8 mg/L - Conductivity:
- 594 µS/cm
- Nominal and measured concentrations:
- - Nominal loading rates: 0 (control) and 100 mg/L (based on a range-finding test)
- Measured concentrations at study initiation (mg carbon/L): see 'Any other information on materials and methods incl. tables'. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 150 mL glass beakers
- Type: Closed, the beakers were covered with transparent lids in order to minimize evaporation and to prevent accidental contamination.
- Fill volume: 100 mL
- Renewal rate of test solution: Once after 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4
- Age: less than 24 hours old (not first brood progeny)
- Feeding during test: no
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The ISO test water recommended by OECD Guideline No. 202 (2004) was used as diluent/solvent of the test item.
- Alkalinity: 66 mg/L as NaHCO3
- Culture medium different from test medium: No
- Intervals of water quality measurement (pH, dissolved oxygen): At start, after 24 and 48 hours. Temperature was measured continuously.
OTHER TEST CONDITIONS
- Photoperiod: 16 h : 8 h
- Lightning: fluorescent
EFFECT PARAMETERS MEASURED: immobilisation, determined after 24 and 48 hours exposure.
PRELIMINARY NON-GLP TEST
- Test loading rates: 0 (control), 1 (diluted), 10 and 100 mg/L.
- Results used to determine the conditions for the definitive study: no immobility was observed in the highest test concentration. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate
- Basis for effect:
- mobility
- Details on results:
- No immobility was observed up to the highest tested concentration. The fresh and 24-hour old test item concentration and the control were homogeneous, clear and colourless.
- Results with reference substance (positive control):
- The test with the reference material potassium dichromate was performed from March 01, 2017 to March 03, 2017.
- 48-h EC50: 0.80 mg/L (95% CI: 0.70 - 0.91 mg/L)
- 48-h EC20: 0.67 mg/L (95% CI: 0.54 – 0.75 mg/L)
- 48-h EC10: 0.61 mg/L (95% CI: 0.46 – 0.69 mg/L)
- 48-h LOEC: 1.00 mg/L
- 48-h NOEC: 0.56 mg/L - Reported statistics and error estimates:
- No statistical analysis was needed.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on materials and methods incl. tables'
- Conclusions:
- The test substance is with high probability not acutely harmful to aquatic invertebrates.
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