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EC number: 204-376-9 | CAS number: 120-20-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from publication.
Data source
Reference
- Reference Type:
- publication
- Title:
- Assay of 855 Test Chemicals in Ten Tester Strains Using a New Modification of the Ames Test for Bacterial Mutagens
- Author:
- Robert E. McMahon,' John C. Cline and Christina Z. Thompson
- Year:
- 1 979
- Bibliographic source:
- CANCER RESEARCH 39, 682-693, March 1979
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Principles of method if other than guideline:
- To evaluate the mutagenic potential for Homoveratrylamine in 10 tester strain of Salmonella Typhimurium and E.coli by Bacterial Mutagen Screen on Gradient Plates with Metabolic Activation.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 4-methoxyphenylacetone
- EC Number:
- 204-578-7
- EC Name:
- 4-methoxyphenylacetone
- Cas Number:
- 122-84-9
- Molecular formula:
- C10H12O2
- IUPAC Name:
- 1-(4-methoxyphenyl)propan-2-one
- Details on test material:
- - Name of test material (IUPAC name): 1-(4-methoxyphenyl)propan-2-one
- Common name: 4-Methoxyphenylacetone
- Molecular formula: C10H12O2
- Molecular weight: 164.203 g/mol
- Smiles notation: c1(ccc(OC)cc1)CC(C)=O
- InChl: 1S/C10H12O2/c1-8(11)7-9-3-5-10(12-2)6-4-9/h3-6H,7H2,1-2H3
- Substance type: Organic
Constituent 1
- Specific details on test material used for the study:
- - Name: 2-(3,4-dimethoxyphenyl)ethan-1-amine
- InChI:1S/C10H15NO2/c1-12-9-4-3-8(5-6-11)7-10(9)13-2/h3-4,7H,5-6,11H2,1-2H3
- Smiles:COc1ccc(CCN)cc1OC
- Name of test material: Homoveratrylamine
- Molecular formula :C10H15NO2
- Molecular weight :181.2335 g/mol
- Substance type:organic
Method
- Target gene:
- Histidine for Salmonella Typhimurium and Tryptophan for E.coli
Species / strain
- Species / strain / cell type:
- bacteria, other: Salmonella Typhimurium strain D3052, TA1538, TA98, C3076,TA1537, G46, TA1535, and TA100. E.coli WP2, and WP2 uVrA
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- other: R-factor plasmids
- Cytokinesis block (if used):
- Not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- Adult male Fischer rats live induced with Aroclor 1254 were used to prepare S9 metabolic activation.
- Test concentrations with justification for top dose:
- 1-1000-µg/ml
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Remarks:
- Plates containing no compound are also prepared to serve as negative controls.
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: -S9;streptozotocin +S9;2- acetylaminofluorene
- Details on test system and experimental conditions:
- Details on test system and conditions
METHOD OF APPLICATION: in agar ( plate incorporation Method)
DURATION
- Exposure duration: 48 hour
NUMBER OF CELLS EVALUATED:
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth
NUMBER OF REPLICATIONS:2 plates /concentration - Rationale for test conditions:
- Preliminary work on sup plemental tests using additional tester strains has been done. One important objective was to find a Salmonella tester strain more sensitive than either strains.
- Evaluation criteria:
- Mutagenic concentration range and minimal inhibitory concentration was observed per plates.
- Statistics:
- Yes
Results and discussion
Test results
- Species / strain:
- bacteria, other: Salmonella Typhimurium strain D3052, TA1538, TA98, C3076,TA1537, G46, TA1535, and TA100. E.coli WP2, and WP2 uVrA
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: No mutagenic effect were observed
Applicant's summary and conclusion
- Conclusions:
- Homoveratrylamine (120-20-7) was tested for its mutagenic potential in 10 tester strain of Salmonella Typhimurium and E.coli by Bacterial Mutagen Screen on Gradient Plates with Metabolic Activation. The test result was considered to be negative both in the presence and absence of metabolic activation.
- Executive summary:
Genetic toxicity in vitro study of Homoveratrylamine was assessed for its possible mutagenic potential . For this purpose was Bacterial Mutagen Screen on Gradient Plates method was performed. The advantage of the gradient method is its high capacity. For example, one compound can be evaluated in 10 tester strains over a 10,000-fold concentration range both with and without metabolic activation using only 8 agar plates. The test substance was exposed to S. typhimurium LT-2: G46 (histidine, missense), TA1535 (G46 with gal-bio-uVrB deletion and LPS deletion), TA100 (G46 with gal-bio-uvrB deletion and LPS deletion with the addition of R-factor pKM1O1), C3076 [histidine, (+) frame-shift],TA1537(C3076with gal-bio-uvrB deletion and LPS deletion), D3052 [histidine , (-F) frame-shift], TA1538 (D3052 with gal-bio-uVrB deletion and LPS deletion), and TA98 (D3052 with gal-bio-uvrB deletion and LPS deletion with the addition of A-factor pKM1O1).E.coli : WP2 (E.coli tryptophan) and WP2 uvrA (E. coli tryptophan with uvrA deletion).4 additional strains are used for supple mental testing. These strains are TA92 (G46 with the addition of A-factor pKM1O1), TA94 (D3052 with the addition of R-factor pKM1O1), CM881 (WP2 with the addition of R factor pKM1O1), and CM891 (WP2 with uVrA deletion and with the addition of A-factor pKM1O1) at the concentration of 1-1000-µg/ml. The test substance was exposed in the presence and absence of metabolic activation. No mutagenic effects were observed. Therefore Homoveratrylamine was considered to be non mutagenic in Salmonella Typhimurium strainD3052, TA1538, TA98, C3076,TA1537, G46, TA1535,TA100 and E.coli WP2, WP2 uVrA in thepresence and absence of metabolic activation. Hence the substance cannot be classified as gene mutant in vitro.
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