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EC number: 205-206-6 | CAS number: 135-62-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Appearance / physical state / colour
Administrative data
- Endpoint:
- appearance / physical state / colour
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: observation made during recent daphnia testing
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Reference
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017/09/04-2017/12/11
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: analytical samples taken at 0 hours and 24 hours from fresh and after 24 and 48 hours from aged test solutions were analysed from control and the test item loading rates of 31.3 and 100 mg/L. Analyses were made only on concentrations were effects were seen, concentrations below were only monitored, not reported.
- Fortified samples: stock solutions of the test item (2450 mg/L, purity not considered) were prepared in acetonitrile. Dilutions (100 mg/L) and (1 mg/L) were prepared in acetonitrile/water (1:1, v/v). The stock solution and the 1 mg/L dilution were used for fortification of 0.006 mg/L and 40 mg/L recovery samples.
- Sample storage (before test): stored deep-frozen (≤ - 18 °C) until analysis - Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: straus, clone 5
- Feeding during test: no feeding during test
- Feeding of culture (before test): three times a week with single cell green algae
- Main test: freshly hatched daphnids less than 24 hours old were used - Test type:
- semi-static
- Water media type:
- other: Elendt M4
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 232 mg/L CaCO3
- Test temperature:
- mean temperature of the control and all test item loading rates: 20.4 ± 0.7 °C
- pH:
- mean pH-value of the untreated control: 7.95 ± 0.05
- Dissolved oxygen:
- mean oxygen concentration: 9.0 ± 0.2 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 100, 31.3, 9.77, 3.05, 0.954 mg/L and control
Test item loading rates (geometric mean measured): 3 mg/L (corresponding to 100), 1.25 mg/L (corresponding to 31.3), other values were only monitored (not reported) and for the control it was not determined - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass vessel
- Type: covered with a glass plate (to reduce evaporation)
- Material, size, headspace, fill volume: 100 mL capacity filled with 50mL of test solution
- No. of organisms: 20 daphnids per test item loading rates (4 replicates of 5 animals each)
- No. of treatment groups: 6 (5 dose rates of the test item and a control)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4
- Culture medium different from test medium: same as Elendt M4
OTHER TEST CONDITIONS
- Photoperiod: 16/8 hours light/dark cycle
EFFECT PARAMETERS MEASURED: Immobilisation
RANGE-FINDING STUDY
- Test concentrations: control, 100, 10 and 1 mg/L
- Sampling points : after 24h and 48h of exposure
- Results used to determine the conditions for the definitive study:
- after 24 h 0% of the daphnids were found to be immobile in the control and up to and including 10 mg/L, 60% immobilisation was observed at 100 mg/L
- after 48 h 0 % of the daphnids were found to be immobile in the control, 10% were found immobile at 1 mg/L and 10 mg/L, 75% were found immobile at 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate, tested at 1.00 and 2.00 mg/L with 20 test organisms per test concentration, at around the same time period as the study
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.31 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.25 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Details on results:
- - Observations in control group: no immobilisation observed during test period
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Test concencentrations: 1 and 2 mg/L (20 organisms per test concentration)
- Relevant effect levels: EC50 (24h) is between 1 and 2 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 for Daphnia magna is 2.31 mg/L (measured, geom. mean).
- Executive summary:
The short-term toxicity to Daphnia magna was determined according to OECD 202 guideline with no deviations. The study was conducted under semi-static conditions. The daphnids were exposed over a period of 48 hours to different nominal concentrations of 100, 31.3, 9.77, 3.05, 0.954 mg/L and a control. The measured concentrations are 3 mg/L (corresponding to 100) and 1.25 mg/L (corresponding to 31.3), the other values were only monitored (not reported) and for the control it was not determined.
The validity criteria were fulfilled.
The EC50 value was determined to be 2.31 mg/L (measured, geom. mean).
Summary of biological results:
a) Results of the test, 24h values
|
nominal test item loading rate [mg/L] |
|||||
|
Control |
0.954 |
3.05 |
9.77 |
31.3 |
100 |
immobilised daphnids after 24 h |
||||||
Group 1 |
0 |
0 |
0 |
0 |
0 |
2 |
Group 2 |
0 |
0 |
0 |
0 |
0 |
4 |
Group 3 |
0 |
0 |
0 |
0 |
0 |
4 |
Group 4 |
0 |
0 |
0 |
0 |
0 |
1 |
Σ |
0 |
0 |
0 |
0 |
0 |
11 |
% |
0 |
0 |
0 |
0 |
0 |
55 |
b) Results of the test, 48h values
|
nominal test item loading rate [mg/L] |
|||||
|
Control |
0.954 |
3.05 |
9.77 |
31.3 |
100 |
immobilised daphnids after 24 h |
||||||
Group 1 |
0 |
0 |
0 |
0 |
0 |
2 |
Group 2 |
0 |
0 |
0 |
0 |
0 |
4 |
Group 3 |
0 |
0 |
0 |
0 |
0 |
4 |
Group 4 |
0 |
0 |
0 |
0 |
0 |
1 |
Σ |
0 |
0 |
0 |
0 |
0 |
11 |
% |
0 |
0 |
0 |
0 |
0 |
55 |
Determined concentration of test substance:
Test item loading rate |
Sampling |
Test substance |
Geometric mean concentration |
Test item concentration |
|||||||
nominal [mg/L] |
[h] |
[mg/L] |
% of nominal |
[%] |
measured[mg/L] |
||||||
Control |
0 fresh |
n.d. |
- |
|
- |
|
|||||
24 aged |
n.d. |
- |
|
||||||||
24 fresh |
n.d. |
- |
|
||||||||
48 aged |
n.d. |
- |
|
||||||||
31.3 |
0 fresh |
2.72 |
9 |
4 |
1.25 |
|
|||||
24 aged |
0.550 |
2 |
|
||||||||
24 fresh |
2.69 |
9 |
|
||||||||
48 aged |
0.569 |
2 |
|
||||||||
100 |
0 fresh |
11.8 |
12 |
3 |
3.00 |
|
|||||
24 aged |
1.59 |
2 |
|
||||||||
24 fresh |
5.69 |
6 |
|
||||||||
48 aged |
1.108 |
1 |
|
||||||||
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- no guideline followed, the results are obtained from observations made during a recent study on Algae testing of the substance.
- GLP compliance:
- no
Test material
- Reference substance name:
- 3-hydroxy-2'-methoxy-2-naphthanilide
- EC Number:
- 205-206-6
- EC Name:
- 3-hydroxy-2'-methoxy-2-naphthanilide
- Cas Number:
- 135-62-6
- Molecular formula:
- C18H14NNaO3
- IUPAC Name:
- 3-hydroxy-2'-methoxy-2-naphthanilide
- Test material form:
- solid
- Details on test material:
- - Batch number: 1366154001
- Purity: 98.7%
- Appearance / colour: solid/ yellow-brown
- Expiry date: 31 May 2019
Constituent 1
Results and discussion
- Physical state at 20°C and 1013 hPa:
- solid
Form / colour / odour
- Key result
- Form:
- solid
- Colour:
- yellow-brown
- Substance type:
- organic
Applicant's summary and conclusion
- Conclusions:
- The test substance is a yellow-brown solid product.
- Executive summary:
The results are obtained from observations made during a recent study on Algae testing of the substance
The substance is a yellow-brown solid product.
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