Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-, N-Me derivatives, molybdatesilicates

Endpoint summary

Description of key information

Biodegradation in water

Biodegradability of Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-, N-Me derivatives, molybdatesilicates (CAS no. 68647 -35 -8) is predicted using QSAR toolbox version 3.4 (2018) with logKow as the primary descriptor. Test substance undergoes 0.55% degradation by BOD in 28 days. Thus, based on percentage degradation, the test chemical Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-, N-Me derivatives, molybdatesilicates was estimated to be not readily biodegradable in water.

Additional information

Biodegradation in water

Predicted data for the target compound Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-, N-Me derivatives, molybdatesilicates (CAS No.68647-35-8) and supporting weight of evidence studies for its closest read across substance were reviewed for the biodegradation end point which are summarized as below:

 

In a prediction done by SSS (2018) using OECD QSAR toolbox version 3.4 with logKow as the primary descriptor, percentage biodegradability of test chemicalBenzenamine, 4 -[(4 -aminophenyl)(4 -imino-2,5 -cyclohexadien-1 -ylidene)methyl]-, N-Me derivatives, molybdatesilicates(CAS No.68647-35-8) was estimated.Test substance undergoes 0.55% degradation by BOD in 28 days. Thus, based on percentage degradation, the test chemical Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-, N-Me derivatives, molybdatesilicates was estimated to be not readily biodegradable in water.

 

In a supporting weight of evidence study from experimental study report (UERL study report, Sustainability Support Services (Europe) AB, Report no. 83803-79-6/01/2018/CBT, 2018) for the read across chemical [4-[[4-anilino-1-naphthyl][4-(dimethylamino)phenyl]methylene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium acetate (CAS no. 83803-79-6),28-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the read across chemical [4-[[4-anilino-1-naphthyl][4 - (dimethylamino)phenyl]methylene]cyclohexa-2,5 -dien-1 -ylidene]dimethylammonium acetate, CAS No. 83803 -79 -6. The test system included control, test item and reference item. The concentration of test and reference item ( Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD28 value of [4 -[[4 -anilino-1 -naphthyl][4 -(dimethylamino)phenyl]methylene]cyclohexa-2,5 -dien-1 -ylidene]dimethylammonium acetate, CAS No. 83803 -79-6 was observed to be 0.52 mgO2/mg. ThOD was calculated as 2.44 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was found to be 21.31%. Based on the results, the test item, under the test conditions, was considered to be not readily biodegradable.

 

For the read across chemical 4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride (CAS no. 6358-36-7), 35-days Closed Bottle test following the OECD guideline 301D to determine the ready biodegradability of the read across chemical 4,4'-carbonimidoylbis[N,Ndiethylaniline] monohydrochloride, CAS No. 6358-36-7 (UERL study report, Sustainability Support Services (Europe) AB, Report no. 6358-36-7/01/2017/CBT, 2017). The test system included control, test item and reference item. The concentration of test and reference item ( Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD35 value of 4,4'-carbonimidoylbis[N,N-diethylaniline] monohydrochloride, CAS No. 6358-36-7 was observed to be 0.52 mgO2/mg. ThOD was calculated as 2.31 mgO2/mg. Accordingly, the % degradation of the test item after 35 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 22.51%. Based on the results, the test item, under the test conditions, was considered to be not readily biodegradable.

 

In an another supporting weight of evidence study from peer reviewed journal (H. Heukelekian and M. C. Rand, 1955) for the read across chemical Basic violet 1 (CAS no. 004 -87 -3), biodegradation experiment was conducted for 5 days for evaluating the percentage biodegradability of read across substance Basic violet 1 using standard dilution method under aerobic conditions at a temperature of 20°C. Sewage was used as a test inoculum. The 5 day BOD value of chemical Basic violet 1 was determined to be 0 g/g. Thus, based on this value, Basic violet 1 is considered to be not readily biodegradable in nature.

 

On the basis of above results for target chemical Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-, N-Me derivatives, molybdatesilicates (from OECD QSAR toolbox version 3.4, 2018) and for its read across substance (from UERL study report and peer reviewed journal), it can be concluded that the test substance Benzenamine, 4-[(4-aminophenyl)(4-imino-2,5-cyclohexadien-1-ylidene)methyl]-, N-Me derivatives, molybdatesilicates can be expected to be not readily biodegradable in nature.