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EC number: 611-390-2 | CAS number: 56467-43-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02.03.2010 to 09.07.2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-propenoic acid, 2-methyl-, 4-benzoylphenyl ester
- EC Number:
- 611-390-2
- Cas Number:
- 56467-43-7
- Molecular formula:
- C17H14O3
- IUPAC Name:
- 2-propenoic acid, 2-methyl-, 4-benzoylphenyl ester
- Reference substance name:
- (4-benzoylphenyl) acetate
- Cas Number:
- 13031-44-2
- Molecular formula:
- C15H12O3
- IUPAC Name:
- (4-benzoylphenyl) acetate
- Test material form:
- solid
Constituent 1
impurity 1
- Specific details on test material used for the study:
- 4-(Methacryloyloxy) bezophenone 97.26 %
Benzophenone acetate: 1.95 %
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- One sample from the freshly prepared stock solution and duplicate samples from the freshly prepared test media ( containing algae) of all test concentrations and from the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentrations during the test period, duplicate samples from the test media of all test concentrations and the control (containing algae) were taken at the
Analysis:
The concentrations of the test item were analysed in the duplicate test media samples from the three highest test concentrations and both sampling times (0 and 72 hours). From the control samples only one of the duplicate samples was analysed from each of both sampling
times. All samples were centrifuged before analysis, in order to remove the algae.
end of the test ( after the 72 hours test period).
All samples were diluted with methanol by a factor of 2 directly after sampling.
Storage of Samples: All samples were measured directly after sampling.
Fortified Samples: 50 mg of the test item were dissolved in 50 mL methanol to obtain a stock solution of 1 g test item/L. Appropriate amounts of this stock solution were diluted with test water to obtain fortified samples at a Ievel of 0.075, 0.2 and 1 mg test item/L.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A water accommodated fraction of 10 mg/L and subsequent dilutions, resulting in nominal concentrations of 1.0, 0.32, 0.10, 0.032 and 0.010 mg test item/L. Additionally, a control was tested in parallel.
The two highest test concentrations of nominal 1.0 and 0.32 mg test item/L correspond to the arithmetic mean measured concentrations of 0.21 and 0.06 mg test item/L.
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Pseudokirchneriella subcapitata, Strain No. 61.81 SAG
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- Calculated water hardness of the test water: 0.24 mmol/L (= 24 mg/L) as CaC03.
- Test temperature:
- 23 °C
- pH:
- 8.1 at test start and 8.8-9.0 at test end
- Nominal and measured concentrations:
- A water accommodated fraction of 10 mg/L and subsequent dilutions, resulting in nominal concentrations of 1.0, 0.32, 0.10, 0.032 and 0.010 mg test item/L. Additionally, a control was tested in parallel.
The two highest test concentrations of nominal 1.0 and 0.32 mg test item/L conespond to the arithmetic mean measured concentrations of 0.21 and 0.06 mg test item/L. - Details on test conditions:
- 25 hours before test start a suspension of a nominal concentration of 10 mg!L was prepared by suspending 10 mg test item into 1000 mL test water. The suspension was stirred for 22 hours in the dark to dissolve as much test item as possible. After a 3 hour cessation period
the dissolved fractions of the test item were taken from the middle of the test vessel. Adequate volumes of this water accommodated fractions were mixed into test water to prepare nominal test
concentrations of 1.0, 0.32, 0.10, 0.032 and 0.010 mg test item/L.
The test media were prepared just before introduction of the algae (= start of the test).
Introduction of Algae:
The test was started (0 hours) by inoculation of a biomass of 5000 algal cells per mL test medium. These cells were taken from an exponentially grnwing pre-culture, which was set up 4 days prior to the test start under the same conditions as in the test.
Replicates:
The test was performed with three replicates per test concentration and six replicates in the control. Volumes of 50 mL of algal suspension per replicate were continuously stirred with magnetic stirrers in 50 mL Erlemneyer flasks. The flasks were covered with
glass dishes and incubated in a water bath.
Blanks:
Additionally, one replicate of each test concentration and of the control was prepared without algae to provide a "blank" for the spectrophotometrical measurements.
Exposure time: 72 h - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.21 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- 95 % conf. limits: n.d.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.21 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95 % conf. limits: n.d.
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 0.21 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 0.21 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.21 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.21 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 0.21 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 0.21 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Reported statistics and error estimates:
- The software used to perform the statistical analysis was ToxRat Professional, Version 2.10, ToxRat® Solutions GmbH, 2009.
Any other information on results incl. tables
Determination of the Test Item: Based on the results of HPLC measurements the concentration of the test item was determined using a calibration curve.
Calibration Range: 0.025 to 0.5 mg test item/L
Linearity of Response: Correlation of peak area of different standard solutions with their corresponding concentrations, using a linear regression
Regression Coefficient: At least 0.9950
Typical Calibration Curve: y = 917153 * x - 7280
Limit of Detection: 0.00075 mg test item/L
Limit of Quantification: 0.0075 mg test item/L
Mean Recovery in the Fortified Samples: 107% (n = 12, RSD 13 %)
Summary of Analytical Results
nominal concentration [mg/L] |
neasured concentration [mg/L]1 |
n |
control |
n.a. |
2 |
0.10 |
n.a. |
4 |
0.32 |
0.056 |
4 |
1.0 |
0.212 |
4 |
|
|
|
1arithmetic mean value of all measured samples per treatment group
RSD relative standard deviation per treatrnent group
n.a. not applicable
n number of analysed samples
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The influence of 4-(Methacryloyloxy)benzophenone on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static dose-response test. The 72-hour NOEC was determined to be at least the limit of water solubility, corresponding to an arithmetic mean measured test concentration of 0.21 mg test item/L. The NOEC might even be higher than this concentration, but concentrations in excess of the limit of solubility have not been tested according to the recommendations of the test guidelines.
The 72-hour LOEC and the 72-hour EC50 were clearly higher than the limit of water solubility, corresponding to an arithmetic mean measured test concentration of 0.21 mg test item/L. - Executive summary:
The influence of 4-(Methacryloyloxy)benzophenone on the growth of the freshwater green algae Pseudokirchneriella subcapitata was assessed in a static dose-response test (OECD Guideline 201 (Alga, Growth Inhibition Test). The 72-hour NOEC was determined to be at least the limit of water solubility, corresponding to an arithmetic mean measured test concentration of 0.21 mg test item/L. The NOEC might even be higher than this concentration, but concentrations in excess of the limit of solubility have not been tested according to the recommendations of the test guidelines. The 72-hour LOEC and the 72-hour EC50 were clearly higher than the limit of water solubility, corresponding to an arithmetic mean measured test concentration of 0.21 mg test item/L.
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